Publications by authors named "Zoey Sharp"
Article Synopsis
- Protein purification is crucial for studying protein structure and developing new therapeutics, leading to a need for improved methods to purify multiple proteins simultaneously.* -
- The researchers created a multi-column plate adapter (MCPA) to efficiently purify proteins using gravity or vacuum, allowing for the simultaneous purification of up to 96 samples with ease.* -
- The MCPA system is adaptable for optimizing different purification parameters and produces enough protein for further analysis, making it a valuable tool in laboratory settings.*
The kinetics of folding and unfolding underlie protein stability and quantification of these rates provides important insights into the folding process. Here, we present a simple high throughput protein unfolding kinetic assay using a plate reader that is applicable to the studies of the majority of 2-state folding proteins. We validate the assay by measuring kinetic unfolding data for the SH3 (Src Homology 3) domain from Actin Binding Protein 1 (AbpSH3) and its stabilized mutants.
View Article and Find Full Text PDF