Occurrence of false positive results for the detection of carbapenemases in carbapenemase-negative Escherichia coli and Klebsiella pneumoniae isolates.

Adequate detection of the production of carbapenemase in Enterobacteriaceae isolates is crucial for infection control measures and the appropriate choice of antimicrobial therapy. In this study, we investigated the frequency of false positive results for the detection of carbapenemases in carbapenemase-negative Escherichia coli and Klebsiella pneumoniae clinical isolates by the modified Hodge test (MHT). Three hundred and one E.

Prevalence of plasmid-mediated quinolone resistance determinants in Citrobacter freundii isolates from Anhui province, PR China.

This study was conducted to detect and analyse the presence of plasmid-mediated quinolone resistance (PMQR) determinants [qnr, aac(6')-Ib-cr and qepA] among Citrobacter freundii isolates from patients in Anhui province, PR China. During 2009-2010, 31 C. freundii strains were collected from various hospital units and patient specimens.

Susceptibility of extended-spectrum-beta-lactamase-producing Enterobacteriaceae according to the new CLSI breakpoints.

In 2010 the Clinical and Laboratory Standards Institute (CLSI) lowered the susceptibility breakpoints of some cephalosporins and aztreonam for Enterobacteriaceae and eliminated the need to perform screening for extended-spectrum β-lactamases (ESBLs) and confirmatory tests. The aim of this study was to determine how many ESBL-producing strains of three common species of Enterobacteriaceae test susceptible using the new breakpoints. As determined with the CLSI screening and confirmatory tests, 382 consecutive ESBL-producing strains were collected at Huashan Hospital between 2007 and 2008, including 158 strains of Escherichia coli, 164 of Klebsiella pneumoniae, and 60 of Proteus mirabilis.

Prevalence of plasmid-mediated quinolone-resistance determinants in Shigella flexneri isolates from Anhui Province, China.

Quinolones are used extensively to treat Shigella flexneri infection, and plasmid-mediated quinolone resistance (PMQR) determinants were recently reported. A total of 26 S. flexneri isolates collected from Anhui province, China, in 2005 were screened for PMQR determinants, bla gene, gyrA and parC genes, by PCR and sequencing.

Protective effects of total flavones of rhododendra against global cerebral ischemia reperfusion injury.

The purpose of this research was to demonstrate the protective effects and possible mechanisms of total flavones of rhododendra (TFR) against global cerebral ischemia reperfusion injury in rats. Global cerebral ischemia/reperfusion injury was caused by four vessel occlusion (bilateral vertebral arteries and bilateral carotid arteries, 4-VO). The electroencephalographic (EEG) changes were recorded.

Investigation of qnr and aac(6')-Ib-cr in Enterobacter cloacae isolates from Anhui Province, China.

Eighty-one Enterobacter cloacae isolates collected from 20 hospitals in Anhui Province, China, from January to August 2005 were screened for plasmid-mediated quinolone resistance determinants by polymerase chain reaction and sequencing. qnrA1, qnrB4, qnrS1, and aac(6')-Ib-cr were present in 6.2% (5/81), 6.

[Detection of the new type of aminoglycoside acetyltransferase gene in Enterobacter cloacae isolates].

Objective: To investigate the prevalence of aac(6')-Ib-cr gene in Enterobacter cloacae isolates.

Methods: PCR and sequencing were performed on 81 strains of Enterobacter cloacae isolated clinically in Anhui province to identify aac (6')-IB-cr gene. Disk diffusion method was used to test the susceptibility of the Enterobacter cloacae isolates with aac (6')-Ib-cr gene to fluoroquinolones, aminoglycosides, and other antimicrobial agents.

Detection of CTX-M-14 extended-spectrum beta-lactamase in Shigella sonnei isolates from China.

Shigellosis is an important cause of acute diarrheal disease and multidrug-resistant phenotype has been reported in S. sonnei. In this study, we investigate the resistance and identify extended-spectrum beta-lactamases (ESBLs) gene in 37 S.

[Identification and expression of blaCTX-M-14 and blaCTX-M-24].

Objective: To identify the ESBL gene and the prevalence in Escherichia coli and Klebsiella pneumoniae strain isolated from Huashan Hospital, Shanghai.

Methods: Isolates were confirmed as an ESBL producing strain by double-disk synergy test and NCCLS Confirmatory Test. Antibiotic susceptibilities were determined by standard agar dilution procedure on Mueller-Hinton agar.

A Klebsiella pneumoniae producing three kinds of class A beta-lactamases encoded by one single plasmid isolated from a patient in Huashan Hospital, Shanghai, China.

A Klebsiella pneumoniae strain was isolated from a sputum specimen of a patient in the intensive care unit in 1999 in Shanghai Huashan Hospital, China. The isolate was confirmed as an extended-spectrum beta-lactamase (ESBL) producing strain by double-disk synergy test. The results of susceptibility test showed that it was resistant to most beta-lactams (including third generation cephalosporins) and non-beta-lactam antimicrobial agents.

[Extended-spectrum beta-lactamase in Klebsiella pneumoniae and Escherichia coli isolates].

Objective: To investigate the prevalence, drug resistance, gene typing, and epidemicity of extended-spectrum beta-lactamases (ESBL) Klebsiella pneumoniae and Escherichia coli isolates.

Methods: 559 strains of K. pneumoniae and 427 strains of E.

Investigation of extended-spectrum beta-lactamase in Klebsiellae pneumoniae and Escherichia coli from China.

Extended-spectrum beta-lactamases (ESBLs) are an increasing cause of resistance to third-generation cephalosporins in Enterobacteriaceae. However, they have not been well studied in China. We investigated the prevalence, resistance, and probable gene type of ESBLs using MICs testing and polymerase chain reaction in 559 Klebsiellae pneumoniae and 427 Escherichia coli isolates collected from patients in Huashan Hospital from 1 January to 31 December 1999.