Real-time quantitative PCR (RT-qPCR) has a high sensitivity and strong specificity, and is widely used in the analysis of gene expression. Selecting appropriate internal reference genes is the key to accurately analyzing the expression changes of target genes by RT-qPCR. To find out the most suitable internal reference genes for studying the gene expression in under abiotic stresses (including drought, salt, and ZnSO treatments), seven different tissues of , as well as the roots, stems, and leaves of under the abiotic stresses were used as test materials, and 15 candidate internal reference genes were screened based on the transcriptome data via RT-qPCR.
View Article and Find Full Text PDFThe color of flowers is one of the main characteristics adopted for plants to attract pollinators to ensure the reproductive success of the plant, they are also important in their ornamental appeal in Narcissus plant. In this study, we identified a NtMYB12 locus encoding an R2R3-MYB transcription factor. Comparative transcriptome analysis of loss- and gain- of NtMYB12 tissue relative to wild-type narcissus showed NtMYB12 was mainly involved in flavonol and phenylpropanoid metabolic pathways.
View Article and Find Full Text PDFThe genetic factors underlying growth traits differ over time points or stages. However, most current studies of phenotypes at single time points do not capture all loci or explain the genetic differences underlying growth trajectories. Hybrid Liriodendron exhibits obvious heterosis and is widely cultivated, although its complex genetic mechanism underlying growth traits remains unknown.
View Article and Find Full Text PDFIntroduction: Wood formation is closely related to lignin biosynthesis. Cinnamoyl-CoA reductase (CCR) catalyzes the conversion of cinnamoyl-CoA to cinnamaldehydes, which is the initiation of the lignin biosynthesis pathway and a crucial point in the manipulation of associated traits. Liriodendron chinense is an economically significant timber tree.
View Article and Find Full Text PDF(Oliv.) Sprague, is a species of evergreen shrub of the genus , family Celastraceae. Here, we extracted the genomic DNA from the leaves of and constructed a paired-end library.
View Article and Find Full Text PDFFront Plant Sci
October 2022
is a relic tree species of the family Magnoliaceae with multiple uses in timber production, landscape decoration, and afforestation. often experiences drought stress in arid areas. However, the molecular basis underlying the drought response of remains unclear.
View Article and Find Full Text PDFClimate change has a crucial impact on the distributions of plants, especially relict species. Hence, predicting the potential impact of climate change on the distributions of relict plants is critical for their future conservation. Liriodendron plants are relict trees, and only two natural species have survived: L.
View Article and Find Full Text PDFBackground: The unique 'mandarin jacket' leaf shape is the most famous trait of and this characteristic gives aesthetic and landscaping value. However, the underlying regulatory mechanism of genes involved in the leaf development of has remained unclear.
Methods: Based on transcriptome data of leaves at different developmental stages from , we identified differentially expression genes (DEGs) functioning in leaf development.
Background: Liriodendron chinense is a distinctive ornamental tree species due to its unique leaves and tulip-like flowers. The discovery of genes involved in leaf development and morphogenesis is critical for uncovering the underlying genetic basis of these traits. Genes in the AP2/ERF family are recognized as plant-specific transcription factors that contribute to plant growth, hormone-induced development, ethylene response factors, and stress responses.
View Article and Find Full Text PDFThe MYB transcription factor family is one of the largest families in plants, and its members have various biological functions. genes are involved in the synthesis of pigments that yield petal colors. plants are widely cultivated as ornamental trees owing to their peculiar leaves, tulip-like flowers, and colorful petals.
View Article and Find Full Text PDFFlower colour and colour patterns are crucial traits for ornamental species; thus, a comprehensive understanding of their genetic basis is extremely significant for plant breeders. The tulip tree ( Linn.) is well known for its flowers, odd leave shape and tree form.
View Article and Find Full Text PDFTaxodium is a genus renowned for its fast growth, good form and tolerance of flooding, salt, alkalinity, disease and strong winds. In this study, a genetic linkage map was constructed using sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR) markers based on an F1 population containing 148 individuals generated from a cross between T. 'Zhongshanshan 302' and T.
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