A feasible protocol to profile bile acids in dried blood spots from rats using a UHPLC-MS/MS method combining a surrogate matrix.

Dried blood spot (DBS) sampling is a promising method for microliter blood sample collection with the advantages of convenient transportation, storage and clinical operations. However, it is challenging to develop an analytical protocol to determine endogenous metabolites, such as bile acids (BAs) in DBSs, due to the low-blood-volume character of DBSs and the complex features of filter paper. Herein, we developed a method of fast ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) to profile and quantify BAs in DBSs.

Evaluating the Effects of Built Environment on Street Vitality at the City Level: An Empirical Research Based on Spatial Panel Durbin Model.

There is evidence that the built environment has an influence on street vitality. However, previous studies seldom assess the direct, indirect, and total effect of multiple environmental elements at the city level. In this study, the features of the street vitality on Xiamen Island are described based on the location-based service Big Data.

Genomics-driven discovery of a new cyclodepsipeptide from the guanophilic fungus .

Two potential non-ribosomal peptide synthetases (NRPSs) were identified in the genome of a guanophilic fungus Amphichorda guana by bioinformatics analysis and gene knockout experiments. Liquid chromatography coupled with mass spectrometry (LC-MS) guided isolation led to the discovery of a new cyclodepsipeptide isaridin H (1) and seven known analogs, desmethylisaridin E (2), isaridin E (3), isariin A (4), iso-isariin B (5), iso-isariin D (6), isariin E (7), and nodupetide (8). The absolute configuration of isaridin H (1) was achieved by Marfey's method.

Comparative Genomic Data Provide New Insight on the Evolution of Pathogenicity in Species.

species are commonly isolated from environmental and clinical samples. As common causes of zoonotic mycosis, species may result in localized or disseminated infections, posing considerable threat to animal and human health. However, the pathogenic profiles of different species varied, in virulence, geographic location and host ranges, which have yet to be explored.