IFNγ-IL12 axis regulates intercellular crosstalk in metabolic dysfunction-associated steatotic liver disease.

Obesity is a major cause of metabolic dysfunction-associated steatohepatitis (MASH) and is characterized by inflammation and insulin resistance. Interferon-γ (IFNγ) is a pro-inflammatory cytokine elevated in obesity and modulating macrophage functions. Here, we show that male mice with loss of IFNγ signaling in myeloid cells (Lyz-IFNγR2) are protected from diet-induced insulin resistance despite fatty liver.

Conserved long noncoding RNA TILAM promotes liver fibrosis through interaction with PML in HSCs.

Background And Aims: Fibrosis is the common end point for all forms of chronic liver injury, and the progression of fibrosis leads to the development of end-stage liver disease. Activation of HSCs and their transdifferentiation into myofibroblasts results in the accumulation of extracellular matrix proteins that form the fibrotic scar. Long noncoding RNAs regulate the activity of HSCs and provide targets for fibrotic therapies.

Conserved long noncoding RNA promotes liver fibrosis through interaction with PML in hepatic stellate cells.

Background & Aims: Fibrosis is the common endpoint for all forms of chronic liver injury, and progression of fibrosis leads to the development of end-stage liver disease. Activation of hepatic stellate cells (HSCs) and their transdifferentiation to myofibroblasts results in the accumulation of extracellular matrix (ECM) proteins that form the fibrotic scar. Long noncoding (lnc) RNAs regulate the activity of HSCs and may provide targets for fibrotic therapies.

Flnc: Machine Learning Improves the Identification of Novel Long Noncoding RNAs from Stand-Alone RNA-Seq Data.

Long noncoding RNAs (lncRNAs) play critical regulatory roles in human development and disease. Although there are over 100,000 samples with available RNA sequencing (RNA-seq) data, many lncRNAs have yet to be annotated. The conventional approach to identifying novel lncRNAs from RNA-seq data is to find transcripts without coding potential but this approach has a false discovery rate of 30-75%.

Green and sustainable cellulose-derived humidity sensors: A review.

Cellulose, as the most abundant natural polysaccharide, is an excellent material for developing green humidity sensors, especially due to its humidity responsiveness as a result of its rich hydrophilic groups. In combination with other components including carbon materials and polymers, cellulose and its derivatives can be used to design high-performance humidity sensors that meet various application requirements. This review summarizes the recent advances in the field of various cellulose-derived humidity sensors, with particular attention paid to different sensing mechanisms including resistance, capacitance, colorimetry and gravity, and so on.

Asymmetrically Patterned Cellulose Nanofibers/Graphene Oxide Composite Film for Humidity Sensing and Moist-Induced Electricity Generation.

The exploration of advanced functional materials from natural resources is significantly important to green and sustainable development. Herein, we design an ultrafast humidity-driven bending response system using asymmetrically patterned cellulose nanofiber (CNF)/graphene oxide (GO) composite films. The CNF/GO composite films are fabricated by vacuum-assisted filtration, followed by a surface imprinting technique.

A multifunctional self-crosslinked chitosan/cationic guar gum composite hydrogel and its versatile uses in phosphate-containing water treatment and energy storage.

A new self-crosslinked composite hydrogel is prepared with chitosan (CS) and cationic guar gum (CGG), based on the imine and acetal chemistry for gelation. The CS/CGG hydrogel exhibits thermal/pH responsiveness, injectability, adhesiveness and good compressive strength. The hydrogel is effective in removing phosphate from wastewater through an adsorption process, during which KHPO is used as a phosphate model.

Frequent mutation of hypoxia-related genes in persistent pulmonary hypertension of the newborn.

Aims: Persistent pulmonary hypertension of the newborn (PPHN) is characterized by sustained high levels of pulmonary vascular resistance after birth with etiology unclear; Arterial blood oxygen saturation of Tibetan newborns at high latitudes is higher than that of Han newborns at low latitudes, suggesting that genetic adaptation may allow sufficient oxygen to confer Tibetan populations with resistance to pulmonary hypertension; We have previously identified genetic factors related to PPHN through candidate gene sequencing; In this study, we first performed whole exome sequencing in PPHN patients to screen for genetic-related factors.

Methods And Results: In this two-phase genetic study, we first sequenced the whole exome of 20 Tibetan PPHN patients and compared it with the published genome sequences of 50 healthy high-altitude Tibetanshypoxia-related genes, a total of 166 PPHN-related variants were found, of which 49% were from 43 hypoxia-related genes; considering many studies have shown that the differences in the genetic background between Tibet and Han are characterized by hypoxia-related genetic polymorphisms, so it is necessary to further verify whether the association between hypoxia-related variants and PPHN is independent of high-altitude life. During the validation phase, 237 hypoxia-related genes were sequenced in another 80 Han PPHN patients living in low altitude areas, including genes at the discovery stage and known hypoxia tolerance, of which 413 variants from 127 of these genes were shown to be significantly associated with PPHN.

A simple microsphere-based mold to rapidly fabricate microwell arrays for multisize 3D tumor culture.

Three-dimensional (3D) tumor has been considered as the best in vitro model for cancer research. In recent years, various methods have been developed to controllable prepare multisize 3D tumors. Nonetheless, reported technologies are still problematic and difficult to produce 3D tumors with highly uniform size and cell content.

Genetic aetiology of early infant deaths in a neonatal intensive care unit.

Background: Congenital anomalies are the leading cause of early neonatal death in neonatal intensive care units (NICUs), but the genetic causes are unclear. This study aims to investigate the genetic causes of infant deaths in a NICU in China.

Methods: Newborns who died in the hospital or died within 1 week of discharge were enrolled from Children's Hospital of Fudan University between January 1, 2015 and December 31, 2017.

Tri-(2-picolyl)amine-modificated triarylborane: Synthesis, photophysical properties and distinguish for cyanide and fluoride anions in aqueous solution.

We designed and synthesized a tri-(2-picolyl) amine (TPA) functionalized triarylborane, 1-(6-(4-(dimesitylboryl)phenyl)pyridin-2-yl)-N,N-bis(pyridin-2-ylmethyl)methanamine (PB2). The photophysical properties of PB2 were thoroughly explored. Moreover, PB2 can capture CN and F in aqueous solution through strong chelation induced by the synergy of a boron atom and metal ion gripped by TPA to display entirely different fluorogenic responses such as fluorescence enhancement for CN and fluorescence quenching for F.

PhenoPro: a novel toolkit for assisting in the diagnosis of Mendelian disease.

Motivation: Whole-exome sequencing (WES) is now being used in clinical practice for the diagnosis of the causal genes of Mendelian diseases. In order to make the diagnosis, however, the clinical phenotypes [e.g.

Colorimetric hydrazine detection and fluorescent hydrogen peroxide imaging by using a multifunctional chemical probe.

Among organic small molecules, hydrogen peroxide (HO) and hydrazine (NHNH) often cause concern because they are widely used in biological and chemical industries. Here, we present a novel probe RH-1 for colorimetric detection of NHNH and fluorescent imaging of HO. In this probe, rhodamine was used as the main skeleton due to its favorable spectroscopic performance and stable absorption.

Whole genome sequencing identifies a novel ALMS1 gene mutation in two Chinese siblings with Alström syndrome.

Background: Alström syndrome is a rare multi-systemic disorder with a broad spectrum of symptoms. This syndrome is characterized by childhood retinal degeneration; sensorineural hearing loss; obesity; type 2 diabetes mellitus; cardiomyopathy; systemic fibrosis; and pulmonary, hepatic, and renal failure.

Case Presentation: A Chinese quartet family with two siblings predominantly affected by cone-rod dystrophy and short stature were recruited.