Temperature-sensitive driving assembled fluorescence hydrogel based dual-mode sensor for adsorbing and detecting of heavy metal cadmium ions in food and water.

The denatured bovine serum albumin (dBSA) is coupled with the CdTe/CdS quantum dot and the resulting CdTe/CdS@dBSA complex is assembled and retained in the poly(n-isopropyl acrylamide) (PNIPAM) hydrogel via regulating temperature and pH to form the CdTe/CdS@dBSA-PNIPAM fluorescence hydrogel substrate, which is able to adsorb and sense cadmium ions (Cd). Based on this fluorescence hydrogel, a fluorescence and colorimetric dual-mode detection system is established to quantitatively detect Cd with a limit of detection (LOD) of 2.88 nM for fluorescence detection and 11.

Homogeneous fluorescence immunoassay based on AuNPs (AgNPs) quenching multicolor QDs@hydrogel beads for the simultaneous ultra-sensitive determination of aflatoxin B and capsaicinoids in food.

A capsaicinoids (CPCs) broad spectrum monoclonal antibody with same recognition ability to capsaicin (CPC), dihydrocapsaicin (DCPC), nordihydrocapsaicin (NDCPC), and N-vanillylnonanamide (NV) is prepared. Chitosan (CS) hydrogel is used as the carrier of multicolor quantum dots (QDs) to prepare fluorescence hydrogel beads, CPCs and aflatoxin B (AFB) antibody are coupled with fluorescence hydrogel beads to prepare signal probes. Using AuNPs (or AgNPs) as fluorescence quenching agent to prepare quenching probes followed forming a fluorescence quenching test system.

Preparation of a capsaicinoids broad spectrum antibody and its application in non-enzyme immunoassay based on DMSNs@PDA@Pt.

Capsaicinoids (CPCs) is a special ingredient with pungent smell in condiments, which can also be used as an exogenetic marker for kitchen waste oil. Development of immunoassay for CPCs remains a challenging due to relatively difficult preparation of the broad-spectrum antibody (Ab). In this work, a broad-spectrum polyclonal antibody (pAb) which can simultaneously recognize capsaicin (CPC), dihydrocapsaicin (DCPC), nordihydrocapsaicin (NDCPC), and N-vanillylnonanamide (N-V) is produced, and a non-enzyme immunoassay (NISA) based on this Ab, dendritic mesoporous silica nanomaterials (DMSNs), polydopamine (PDA), and high catalytic efficiency of Pt nanoparticles to prepare signal probe (DMSNs@PDA@Pt) is established.

Colorimetric and photothermal dual-mode immunosensor based on TiCT/AuNPs nanocomposite with enhanced peroxidase-like activity for ultrasensitive detection of zearalenone in cereals.

A novel peroxidase-like nanozyme has been constructed by decorating two-dimensional TiCT nanosheets (TiCT NSs) with gold nanoparticles (AuNPs) to develop a colorimetric and photothermal dual-mode immunosensor. The TiCT/AuNPs nanocomposite-catalyzed 3,3',5,5'-tetramethylbenzidine (TMB)-HO reaction system produces the one-electron oxidation product of TMB (oxTMB), which exhibits color change and strong near-infrared (NIR) laser-driven photothermal effect at 808 nm laser irradiation. Given these characteristics, the developed immunosensor achieves ultrasensitive dual-mode detection of zearalenone (ZEN) by measuring colorimetric and photothermal signals with a microplate reader and a portable infrared thermometer, respectively.

Mutant RB1 enhances therapeutic efficacy of PARPis in lung adenocarcinoma by triggering the cGAS/STING pathway.

Poly (ADP-ribose) polymerase inhibitors (PARPis) are approved for cancer therapy according to their synthetic lethal interactions, and clinical trials have been applied in non-small cell lung cancer. However, the therapeutic efficacy of PARPis in lung adenocarcinoma (LUAD) is still unknown. We explored the effect of a mutated retinoblastoma gene (RB1) on PARPi sensitivity in LUAD.

Synthetic viability induces resistance to immune checkpoint inhibitors in cancer cells.

Background: Immune checkpoint inhibitors (ICI) have revolutionized the treatment for multiple cancers. However, most of patients encounter resistance. Synthetic viability (SV) between genes could induce resistance.

Fluorescence immunoassay for simultaneous detection typical β-agonists in animal derived food using blue-green upconversion nanoparticles as labels.

Common typical β-agonists mainly include ractopamine (RAC), salbutamol (SAL), and clenbuterol (CLB). In view of the harm to human health causes by the ingestion of animal derived food containing β-agonists, and a series of regulations have been issued to restrict the usage of β-agonists as growth promoters. In this work, a fluorescence immunoassay is developed for the simultaneous detection of typical β-agonists based on blue-green upconversion nanoparticles (UCNPs) combine with magnetic separation.

A fluorescence immunoassay based on GSH destroying MnO@QDs for the simultaneous ultrasensitive detection of four mycotoxins in cereals.

A novel fluorescence immunoassay based on MnO nanoflowers loading multicolor quantum dots and glutathione destroying MnO nanoflowers to release quantum dots combined with magnetic separation is developed for rapid, ultra-sensitive, and simultaneous quantitative detection of ochratoxin A, aflatoxin B, fumonisin B, and zearalenone in cereal samples. The test linear range of assay is from 0.001 to 200 μg L.

Operational and Financial Impacts of Digital Health Technology: A Study on Canadian Healthcare System during the COVID-19 Pandemic.

During COVID-19, hospital capacity was significantly reduced to limit the spread of the pandemic. The limitations affected the efficiency of service delivery. We examined the effects of pandemic-related challenges on patient experience and hypothesize that digital health implementation increased patient satisfaction.

Freeze-synthesized bio-barcode immunoprobe based multiplex fluorescence immunosensor for simultaneous determination of four nitrofuran metabolites.

Here we have reported a simple and sensitive bio-barcode immunosensor for simultaneous detection of 3-amino-2-oxazolidinone (AOZ), 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), 1-aminohydantoin (AHD), and semicarbazide (SEM) in aquatic products. According to freeze-thaw strategy, four fluorophores (FAM, HEX, ROX, Cy5) labeled single-stranded DNA (ssDNA) were conjugated onto the surface of gold nanoparticles (AuNPs) with corresponding four nitrofuran metabolites monoclonal antibodies (mAbs) for forming four bio-barcode fluorescence immunoprobes. The fluorescence of immunoprobes was quenched by AuNPs.