Mitochondrial Gene Expression of Three Different Dragonflies Under the Stress of Chlorpyrifos.

Chlorpyrifos (CPF) is an organophosphate insecticide that is extensively utilized globally due to its effectiveness against over 200 pest species. CPF exhibits its toxicity primarily through the inhibition of the acetylcholinesterase (AChE) enzyme, while mitochondrial damage and dysfunction have also been observed. The present study quantified the transcript levels of mitochondria protein-coding genes (mtPCGs) using quantitative real-time polymerase chain reaction () in samples of larvae of three dragonfly species (, , and ) under different levels of CPF stress.

Engineering n-Type and p-Type BiOI Nanosheets: Influence of Mannitol on Semiconductor Behavior and Photocatalytic Activity.

Photocatalytic technology holds significant promise for sustainable development and environmental protection due to its ability to utilize renewable energy sources and degrade pollutants efficiently. In this study, BiOI nanosheets (NSs) were synthesized using a simple water bath method with varying amounts of mannitol and reaction temperatures to investigate their structural, morphological, photoelectronic, and photocatalytic properties. Notably, the introduction of mannitol played a critical role in inducing a transition in BiOI from an n-type to a p-type semiconductor, as evidenced by Mott-Schottky (M-S) and band structure analyses.

Vertical distribution characteristics of microplastics and bacterial communities in the sediment columns of Jianhu lake in China.

Microorganisms change the properties of microplastics, at the same time, microplastics can also affect the distribution of microorganisms. To investigate this issue, we chose to study Jianhu Lake, a plateau lake in southwestern China, by collecting data at three sampling locations. The microplastics and bacterial communities in the sediment columns of Jianhu Lake were sampled within a 0 to 60 cm profile, and the basic characteristics of microplastic abundance, shape, color, size, and polymer type were determined accordingly, via their collection, separation, extraction, and identification.

Dual-functional CuO/g-CN heterojunctions: a high-performance SERS sensor and photocatalytic self-cleaning system for water pollution detection and remediation.

This study introduces a multifunctional device based on CuO/g-CN monitoring and purification p-n heterojunctions (MPHs), seamlessly integrating surface-enhanced Raman scattering (SERS) detection with photocatalytic degradation capabilities. The SERS and photocatalytic performances of the CuO in various morphologies, g-CN nanosheets (NSs) and CuO/g-CN MPHs with different g-CN mass ratios were systematically evaluated, with a particular emphasis on the CuO/g-CN-0.2 MPH, where g-CN constituted 20% of the total mass.

Photocatalytic Enhancement and Recyclability in Visible-Light-Responsive 2D/2D g-CN/BiOI p-n Heterojunctions via a Z-Scheme Charge Transfer Mechanism.

With the intensification of the energy crisis and the growing concern over environmental pollution, particularly the discharge of organic dye pollutants in industrial wastewater, photocatalytic degradation of these contaminants using solar energy has emerged as an effective, eco-friendly solution. In this study, we successfully synthesized 2D/2D g-CN/BiOI p-n heterojunctions via a simple precipitation method and a high-temperature calcination method. The unique 2D structures of g-CN nanosheets (NSs) and BiOI NSs, coupled with the synergistic effect between the two materials, significantly enhanced the photocatalytic degradation performance of the heterojunctions under simulated sunlight.

Roseobacter sinensis sp. nov., a marine bacterium capable to synthesize arachidonic acid.

Strain WL0113 was isolated from surface seawater of the coast of Lianyungang, Jiangsu province, PR China. Strain WL0113 shared highest 16S rRNA gene sequence similarity with Roseobacter insulae YSTF-M11 (98.8%), followed by R.

Heterocycle-functional steroidal derivatives: Design, synthesis, bioevaluation and SARs of steroidal pyrazolo[1,5-a]pyrimidines as potential ALK inhibitors.

Two series of heterocyclic steroidal pyrazolo[1,5-a]pyrimidines derived from dehydroepiandrosterone (DHEA) and epiandrosterone (EPIA) were designed and synthesized, and these compounds were screened for their potential antiproliferation activities. The preliminary bioassay indicated that some of target compounds exhibited significantly good antiproliferation activities against human melanoma cell line (A875) and human hepatocellular carcinoma (Huh-7) cell lines compared with 5-fluorouracil (5-FU), and some of which present good antiproliferative activities as potential ALK inhibitors. The detailed analysis of structure-activity relationships (SARs) based on the inhibition activities, kinase assay, and molecular docking demonstrated that the antiproliferation activities of these steroidal pyrazolo[1,5-a]pyrimidine might be affected by the β-hydroxyl group of steroidal scaffold and the N atom of pyridine heterocycle.

Community structure and carbon metabolism functions of bacterioplankton in the Guangdong coastal zone.

Unlabelled: Coastal ecosystems are an important region for biogeochemical cycling, are a hotspot of anthropogenic disturbance and play a crucial role in global carbon cycling through the metabolic activities of bacterioplankton. Bacterioplankton can be broadly classified into two lifestyles: free-living (FL) and particle-attached (PA). However, how coastal bacterioplankton the community structure, co-occurrence networks and carbon metabolic functions with different lifestyles are differentiated is still largely unknown.

Cultivation strategies for prokaryotes from extreme environments.

The great majority of microorganisms are as-yet-uncultivated, mostly found in extreme environments. High-throughput sequencing provides data-rich genomes from single-cell and metagenomic techniques, which has enabled researchers to obtain a glimpse of the unexpected genetic diversity of "microbial dark matter." However, cultivating microorganisms from extreme environments remains essential for dissecting and utilizing the functions of extremophiles.

sp. nov., a novel bacterium isolated from the South China Sea.

Two Gram-stain-negative strains, designed SYSU M86414 and SYSU M84420, were isolated from marine sediment samples of the South China Sea (Sansha City, Hainan Province, PR China). These strains were aerobic and could grow at pH 6.0-8.

Stomatohabitans albus gen. nov., sp. nov., an oral living facultative anaerobic actinobacteria isolated form Steller sea lion, and proposal of Stomatohabitantaceae fam. nov. and Stomatohabitantales ord. nov.

Two novel actinobacteria, designated as SYSU M7M538 and SYSU M7M531, were isolated from oral of Eumetopias jubatus in Zhuhai Chimelong Ocean Kingdom, China. The cells of these microorganisms stained Gram-positive and were rod shaped. These strains were facultative anaerobic, and catalase-positive.

Bearing-Based Robust Formation Tracking Control of Underactuated AUVs With Optimal Parameter Tuning.

This article investigates the control problem of bearing-based formation tracking for underactuated autonomous underwater vehicles (AUVs) considering actuator constraints and unknown disturbances. A leader-follower structure is adopted, where the leaders move with an unknown reference velocity. For the followers, an integrated strategy is proposed, which includes i) a bearing-based control method composed of a reference velocity estimator, a virtual velocity for achieving the desired formation, and an adaptive robust formation controller to track the virtual velocity under disturbances; and ii) a parameter tuning method based on control parameterization approaches and heuristic algorithms.

Different roles of host and habitat in determining the microbial communities of plant-feeding true bugs.

Background: The true bugs (Heteroptera) occupy nearly all of the known ecological niches of insects. Among them, as a group containing more than 30,000 species, the phytophagous true bugs are making increasing impacts on agricultural and forestry ecosystems. Previous studies proved that symbiotic bacteria play important roles in these insects in fitting various habitats.

Alsobacter ponti sp. nov., a novel denitrification and sulfate reduction bacterium isolated from Pearl River sediment.

A Gram-staining negative, aerobic, motile, and short rods strain, designated SYSU M60028, was isolated from a Pearl River sediment sample in Guangzhou, Guangdong, China. The isolate could be able to grow at pH 6.0-8.

Characterization of a reassortant H11N9 subtype avian influenza virus isolated from spot-billed duck in China.

H11N9 viruses in wild birds might have provided the NA gene of human H7N9 virus in early 2013 in China, which evolved with highly pathogenic strains in 2017 and caused severe fatalities. To investigate the prevalence and evolution of the H11N9 influenza viruses, 16,781 samples were collected and analyzed during 2016-2020. As a result, a novel strain of influenza A (H11N9) virus with several characteristics that increase virulence was isolated.

sp. nov., a novel bacterium isolated from Pearl River Estuary.

A Gram-staining-positive, aerobic, motile, and rod-shaped strain, designated SYSU M60031, was isolated from a Pearl River Estuary sediment sample, Guangzhou, Guangdong, China. The isolate could grow at pH 5.0-8.

Robot-Assisted Percutaneous Balloon Compression in Elderly Patients with Trigeminal Neuralgia.

Objective: To investigate the clinical effects of percutaneous balloon compression (PBC) for trigeminal neuralgia in elderly patients with the assistance of a neurosurgical navigation and positioning planning system (referred to hereafter as the robot).

Methods: We performed a retrospective analysis of 11 patients with trigeminal neuralgia. Preoperative TOF MRA, T2WI-SPACE, and thin-slice CT scans were performed, and the volume of Meckel's Cave was calculated by multi-modal image fusion on a workstation.

Novel Steroidal[17,16-]pyrimidines Derived from Epiandrosterone and Androsterone: Synthesis, Characterization and Configuration-Activity Relationships.

Two series of novel steroidal[17,16-]pyrimidines derived from natural epiandrosterone and androsterone were designed and synthesized, and these compounds were screened for their potential anticancer activities. The preliminary bioassay indicated that some of these prepared compounds exhibited significantly good cytotoxic activities against human gastric cancer (SGC-7901), lung cancer (A549), and hepatocellular liver carcinoma (HepG2) cell lines compared with 5-fluorouracil (5-FU), epiandrosterone, and androsterone. Especially the respective pairs from epiandrosterone and androsterone showed significantly different inhibitory activities, and the possible configuration-activity relationships have also been summarized and discussed based on kinase assay and molecular docking, which indicated that the inhibition activities of these steroidal[17,16-]pyrimidines might obviously be affected by the configuration of the hydroxyl group in the part of the steroidal scaffold.

Symbiotic Microorganisms and Their Different Association Types in Aquatic and Semiaquatic Bugs.

True bugs (Hemiptera, suborder Heteroptera) constitute the largest suborder of nonholometabolous insects and occupy a wide range of habitats various from terrestrial to semiaquatic to aquatic niches. The transition and occupation of these diverse habitats impose various challenges to true bugs, including access to oxygen for the aquatic species and plant defense for the terrestrial phytophagans. Although numerous studies have demonstrated that microorganisms can provide multiple benefits to terrestrial host insects, a systematic study with comprehensive higher taxa sampling that represents aquatic and semiaquatic habitats is still lacking.

Acaricidal Activity and Field Efficacy Analysis of the Potential Biocontrol Agent NBIF-001 against Spider Mites.

In recent years, spider mites have caused considerable economic losses to global agriculture. However, currently available management strategies are limited because of the rapid development of resistance. In this study, NBIF-001 was isolated and evaluated for its acaricidal activity.

Bearing-Based Formation Tracking Control With Time-Varying Velocity Estimation.

This article studies the bearing-based formation tracking control problem of multiple double-integrator agents. A leader-following structure, where the leader moves with the reference dynamics, is adopted. Different from the existing methods, which require complete information of the time-varying reference velocity, in this article, only the time-varying reference orientation information is known by part of the followers and the amplitude of the reference velocity is unknown.