High driving pressure ventilation induces pulmonary hypertension in a rabbit model of acute lung injury.

Background: Mechanical ventilation may cause pulmonary hypertension in patients with acute lung injury (ALI), but the underlying mechanism remains elucidated.

Methods: ALI was induced in rabbits by a two-hit injury, i.e.

Iodine and freeze-drying enhanced high-resolution MicroCT imaging for reconstructing 3D intraneural topography of human peripheral nerve fascicles.

Background: The precise annotation and accurate identification of the topography of fascicles to the end organs are prerequisites for studying human peripheral nerves.

New Method: In this study, we present a feasible imaging method that acquires 3D high-resolution (HR) topography of peripheral nerve fascicles using an iodine and freeze-drying (IFD) micro-computed tomography (microCT) method to greatly increase the contrast of fascicle images.

Results: The enhanced microCT imaging method can facilitate the reconstruction of high-contrast HR fascicle images, fascicle segmentation and extraction, feature analysis, and the tracing of fascicle topography to end organs, which define fascicle functions.