Club cell secretory protein 16 is a potential biomarker for silica-induced pulmonary fibrosis.

This study was conducted to investigate the changes of Club cell protein 16 (CC16) and surfactant protein D (SP-D) levels in serum and bronchoalveolar lavage fluid (BALF) in silicotic rats and to explore their potential as early biomarkers for silicosis. Pulmonary fibrosis models of rats were constructed by exposing them to silica particles. BALF and serum were collected to determine CC16 and SP-D levels using enzyme-linked immunosorbent assay (ELISA) at different times after the exposure.

Identification and Characterization of miRNAs and Their Predicted mRNAs in the Larval Development of Pearl Oyster Pinctada fucata.

As an important economic shellfish, the pearl oyster, Pinctada fucata, and its larvae are an ideal model for studying molecular mechanisms of larval development in invertebrates. Larval development directly affects the quantity and quality of pearl oysters. MicroRNAs (miRNAs) may play important roles in development, but the effects of miRNA expression on P.

[Determination of 14 pesticide residues in crucian carp using on-line gel permeation chromatography-multi gas chromatography/mass spectrometry].

A new method was developed for the determination of pesticide residues in crucian carp using on-line gel permeation chromatography-multi gas chromatography/mass spectrometry (GPC-MDGC/MS). The pesticide residues were extracted with cyclohexane-ethyl acetate (1:1, v/v) for twice. After being frozen, the extract was filtered by 0.

Electrochemistry of hemoglobin entrapped in a Nafion/nano-ZnO film on carbon ionic liquid electrode.

A stable composite film composed of the ionomer Nafion, the ZnO nanoparticle and the protein hemoglobin was cast on the surface of an ionic liquid modified carbon paste electrode (CILE) to establish a modified electrode denoted as Nafion/nano-ZnO/Hb/CILE. UV-vis and FT-IR spectrum showed that hemoglobin in the film retained its native conformation. The electrochemical behaviors of hemoglobin entrapped in the film were carefully investigated with cyclic voltammetry.