Genetic variation in Arabidopsis thaliana reveals the existence of natural heat resilience factors for meiosis.

Heat interferes with multiple meiotic processes, leading to genome instability and sterility in flowering plants, including many crops. Despite its importance for food security, the mechanisms underlying heat tolerance of meiosis are poorly understood. In this study, we analyzed different meiotic processes in the Arabidopsis (Arabidopsis thaliana) accessions Columbia (Col) and Landsberg erecta (Ler), their F1 hybrids, and the F2 offspring under heat stress (37°C).

Wide FOV metalens for near-infrared capsule endoscopy: advancing compact medical imaging.

This study presents the design, fabrication, and characterization of a wide field-of-view (FOV) metalens optimized for capsule endoscopy. The metalens achieved a 165° FOV with a high modulation transfer function (MTF) of 300 lines per millimeter (lp/mm) across the entire FOV, operating in the near-infrared (NIR) narrow-bandpass imaging at 940 nm. The performance of the metalens-based system is evaluated using two bandwidths, 12 nm and 32 nm, showing MTF values of 0.

The Critical Role of Phenylpropanoid Biosynthesis Pathway in Lily Resistance Against Gray Mold.

Gray mold caused by is one of the most determinative factors of lily growth and has become a major threat to lily productivity. However, the nature of the lily interaction remains largely unknown. Here, comparative transcriptomic and metabolomic were used to investigate the defense responses of resistant ('Sorbonne') and susceptible ('Tresor') lily cultivars to infection at 24 hpi.

Event-Triggered Data-Driven Security Formation Control for Quadrotors Under Denial-of-Service Attacks and Communication Faults.

In this article, the security formation control problem is investigated for underactuated quadrotors involving nonlinear coupled dynamics, subject to denial-of-service (DoS) attacks and uncertain communication faults. A security formation control method is proposed, including a distributed resilient observer and a hierarchical data-driven controller. The observer with an adaptive event-triggered mechanism is developed to restrain the influence of DoS and communication faults on interaction information among quadrotors, and Zeno behavior of all observers can be avoided.

Ultraviolet attenuates centromere-mediated meiotic genome stability and alters gametophytic ploidy consistency in flowering plants.

Ultraviolet (UV) radiation influences development and genome stability in organisms; however, its impact on meiosis, a special cell division essential for the delivery of genetic information across generations in eukaryotes, has not yet been elucidated. In this study, by performing cytogenetic studies, we reported that UV radiation does not damage meiotic chromosome integrity but attenuates centromere-mediated chromosome stability and induces unreduced gametes in Arabidopsis thaliana. We showed that functional centromere-specific histone 3 (CENH3) is required for obligate crossover formation and plays a role in the protection of sister chromatid cohesion under UV stress.

Identification and Expression Analysis of Putative Sugar Transporter Gene Family during Bulb Formation in Lilies.

Sugar transporters play important roles in plant growth and development, flowering and fruiting, as well as responses to adverse abiotic and biotic environmental conditions. Lilies ( spp.) are some of the most representative ornamental bulbous flowers.

Full-length transcriptome analysis revealed that 2,4-dichlorophenoxyacetic acid promoted bulblet initiation in lily by affecting carbohydrate metabolism and auxin signaling.

Bulblet initiation, including adventitious bud initiation and bulblet formation, is a crucial process for lily and other bulbous flowers that are commercially propagated by vegetative means. Here, by a hybrid strategy combining Pacific Biosciences (PacBio) full-length sequencing and Illumina RNA sequencing (RNA-seq), high-quality transcripts of () and its variety, var. giganteum (), during bulblet initiation were obtained.

Identifying, Resolving, and Quantifying Anisotropy in ReS Nanomechanical Resonators.

As an emerging two-dimensional semiconductor, rhenium disulfide (ReS ) is renowned for its strong in-plane anisotropy in electrical, optical, and thermal properties. In contrast to the electrical, optical, optoelectrical, and thermal anisotropies that are extensively studied in ReS , experimental characterization of mechanical properties has largely remained elusive. Here, it is demonstrated that the dynamic response in ReS nanomechanical resonators can be leveraged to unambiguously resolve such disputes.

ATM-mediated double-strand break repair is required for meiotic genome stability at high temperature.

In eukaryotes, meiotic recombination maintains genome stability and creates genetic diversity. The conserved Ataxia-Telangiectasia Mutated (ATM) kinase regulates multiple processes in meiotic homologous recombination, including DNA double-strand break (DSB) formation and repair, synaptonemal complex organization, and crossover formation and distribution. However, its function in plant meiotic recombination under stressful environmental conditions remains poorly understood.

Meroterpenoids and Steroids from the Marine-Derived Fungus sp. ZYX-Z-16.

Marine fungi can metabolize structurally diverse active components, and have become an important source of drug lead molecules. In the present study, the chemical investigation on the EtOAc extract of the fermentation broth of the marine-derived fungus sp. ZYX-Z-16 led to the isolation of eight meroterpenoids (-), including two undescribed ones, together with ten ergostane steroid analogues (-).

Emotional-Health-Oriented Urban Design: A Novel Collaborative Deep Learning Framework for Real-Time Landscape Assessment by Integrating Facial Expression Recognition and Pixel-Level Semantic Segmentation.

Emotional responses are significant for understanding public perceptions of urban green space (UGS) and can be used to inform proposals for optimal urban design strategies to enhance public emotional health in the times of COVID-19. However, most empirical studies fail to consider emotion-oriented landscape assessments under dynamic perspectives despite the fact that individually observed sceneries alter with angle. To close this gap, a real-time sentimental-based landscape assessment framework is developed, integrating facial expression recognition with semantic segmentation of changing landscapes.

Comparative transcriptome and metabolome analyses identified the mode of sucrose degradation as a metabolic marker for early vegetative propagation in bulbs of Lycoris.

Vegetative propagation (VP) is an important practice for production in many horticultural plants. Sugar supply constitutes the basis of VP in bulb flowers, but the underlying molecular basis remains elusive. By performing a combined sequencing technologies coupled with ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry approach for metabolic analyses, we compared two Lycoris species with contrasting regeneration rates: high-regeneration Lycoris sprengeri and low-regeneration Lycoris aurea.

Integrative Comparative Assessment of Cold Acclimation in Evergreen and Deciduous Iris Species.

Cold acclimation (CA) is a strategy which plants have evolved to increase freezing tolerance. Global climate change could obstruct CA and raise the probability of winter injury, especially for evergreens. Hence, understanding the regulatory mechanism of CA is crucial to improve freezing tolerance in evergreen plants.

Expression analysis of TLR signaling pathway genes under lipopolysaccharide-induced and F17-infected sheep intestinal epithelial cells.

() F17 is one of the main pathogens causing diarrhea in young livestock. The specific F17 fimbriae and lipopolysaccharide (LPS) in the surface components of F17 induces immune activation interacting with the intestinal epithelial cells (IECs)-expressed innate immune toll-like receptors (TLRs) signaling pathway. In this study, the expression patterns of eight canonical genes from the TLR signaling pathway (, , , , , , and ) were analyzed in LPS-induced IECs, F17-infected IECs and ileum tissue of F17-infected lambs.

Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With F17.

It has long been recognized that enterotoxigenic (ETEC) is the major pathogen responsible for vomiting and diarrhea. F17, a main subtype of ETEC, is characterized by high morbidity and mortality in young livestock. However, the transcriptomic basis underlying F17 infection has not been fully understood.

Effects of di-n-butyl phthalate on aerobic composting process of agricultural waste: Mainly based on bacterial biomass and community dynamics analysis.

Phthalic acid esters (PAEs) pollution has become a major environmental problem in agricultural waste composting. However, little information was available about the how the PAEs alter microbial processes during composting. This study investigated the effects of di-n-butyl phthalate (DBP) on bacterial biomass and community dynamics during composting.

Non-Coding Transcriptome Provides Novel Insights into the F17 Susceptibility of Sheep Lamb.

It has long been recognized that enterotoxigenic (ETEC) is the major pathogen responsible for vomiting and diarrhea. F17, a main subtype of ETEC, is characterized by high morbidity and mortality in young livestock. However, the transcriptomic basis underlying F17 infection has not been fully understood.

A Compact Optical MEMS Pressure Sensor Based on Fabry-Pérot Interference.

Pressure sensors have important prospects in wind pressure monitoring of transmission line towers. Optical pressure sensors are more suitable for transmission line towers due to its anti-electromagnetic interference. However, the fiber pressure sensor is not a suitable choice due to expensive and bulky.

Interfered chromosome pairing at high temperature promotes meiotic instability in autotetraploid Arabidopsis.

Changes in environmental temperature affect multiple meiotic processes in flowering plants. Polyploid plants derived from whole-genome duplication (WGD) have enhanced genetic plasticity and tolerance to environmental stress but face challenges in organizing and segregating doubled chromosome sets. In this study, we investigated the impact of increased environmental temperature on male meiosis in autotetraploid Arabidopsis (Arabidopsis thaliana).

Early Sucrose Degradation and the Dominant Sucrose Cleavage Pattern Influence Bulblet Regeneration In Vitro.

Bulblet formation and development determine the quantitative and qualitative traits, respectively, of bulb yield for most flowering bulbs. For species, however, the underlying molecular mechanism remains elusive. Here, clonal bulblets of () derived from the same probulb were used as explants to establish efficient and inefficient in vitro regeneration systems by adjusting the 6-benzyladenine (BA) concentrations in media.

Robot-aided fN∙m torque sensing within an ultrawide dynamic range.

In situ scanning electron microscope (SEM) characterization have enabled the stretching, compression, and bending of micro/nanomaterials and have greatly expanded our understanding of small-scale phenomena. However, as one of the fundamental approaches for material analytics, torsion tests at a small scale remain a major challenge due to the lack of an ultrahigh precise torque sensor and the delicate sample assembly strategy. Herein, we present a microelectromechanical resonant torque sensor with an ultrahigh resolution of up to 4.

Molecular cloning, characterization and expression analysis of three key starch synthesis-related genes from the bulb of a rare lily germplasm, var. .

Starch is the predominant compound in bulb scales, and previous studies have shown that bulblet development is closely associated with starch enrichment. However, how starch synthesis affects bulbification at the molecular level is unclear. In this study, we demonstrate that var.

Change in Sucrose Cleavage Pattern and Rapid Starch Accumulation Govern Lily Shoot-to-Bulblet Transition .

In bulb crops, bulbing is a key progress in micropropagation and is the feature that most distinguishes bulbous crops from other plants. Generally, bulbing involves a shoot-to-bulblet transition; however, the underlying mechanism remains elusive. We explored this process by tracking the shoot-to-bulblet transition under different culture conditions.

Design and Fabrication of Micro Hemispheric Shell Resonator with Annular Electrodes.

Electrostatic driving and capacitive detection is widely used in micro hemispheric shell resonators (HSR). The capacitor gap distance is a dominant factor for the initial capacitance, and affects the driving voltage and sensitivity. In order to decrease the equivalent gap distance, a micro HSR with annular electrodes fabricated by a glassblowing method was developed.