Effects of PCNL under the guidance of hologram technology on stress response and renal injury factors in patients with complex kidney stones.

Background: To investigate the effect of percutaneous nephrolithotomy (PCNL) guided by holographic image technology on stress response and renal injury factors in patients with complex renal calculi.

Methods: A retrospective analysis was conducted on the clinical data of 70 patients admitted to our hospital between August 2022 and June 2023 who had complex kidney stones. The patients were divided into two groups, namely, group A and group B, based on whether they received guidance from preoperative holographic imaging technology.

ZnO nanocrystals anchored graphene: in situ solvothermal synthesis and enhanced photocatalytic performance.

ZnO nanocrystals anchored graphene composite (ZnO/G) was synthesized by a facile in situ solvothermal route. The nanocomposite was characterized by X-ray diffraction (XRD), scanning electron spectroscopy (SEM), transmission electron microscopy (TEM) and Raman spectra (RS). The results indicated that the ZnO particles with an average diameter of ca.

Visualizing the electrochemical reaction of ZnO nanoparticles with lithium by in situ TEM: two reaction modes are revealed.

The lithiation reaction of ZnO as an anode in a lithium-ion battery (LIB) is unclear. The electrochemical behavior of ZnO was investigated inside a transmission electron microscope (TEM) by constructing a nano-LIB using an individual ZnO/graphene sheet as the electrode. The lithiation reaction of ZnO/graphene was monitored by simultaneous determination of the structure with high-resolution TEM, electron diffraction pattern and electron energy-loss spectroscopy.

Hypermethylation-modulated downregulation of RASSF1A expression is associated with the progression of esophageal cancer.

Background And Aims: Chromosome 3p21 is an important locus harboring critical tumor suppressor genes (TSGs) implicated in the pathogenesis of multiple tumors including esophageal carcinoma (EC). Aberrant promoter methylation is a fundamental mechanism of inactivation of TSGs in cancer. RASSF1A, a candidate tumor suppressor gene, recently cloned from the lung tumor locus at 3p21.

Aberrant methylation of different DNA repair genes demonstrates distinct prognostic value for esophageal cancer.

Background: DNA mismatch repair (MMR) deficiency results in a strong mutator phenotype and high-frequency microsatellite instability (MSI-H), which are the hallmarks of many tumors.

Aim: The objective of this study is to investigate the promoter CpG island methylation status of mismatch repair genes human mutL homolog 1 (hMLH1), human mutS homolog 2 (hMSH2), and O(6)-methylguanine-DNA methyltransferase (MGMT) in esophageal squamous cell carcinoma (ESCC) and its roles in alkylating agents chemotherapy.

Methods: Real-time methylation-specific polymerase chain reaction (PCR) (real-time MSP) was employed to detect promoter CpG island methylation of the hMLH1, hMSH2, as well as MGMT genes in 235 surgical tumor tissue samples from ESCC patients and their corresponding normal tissue samples.

Hypermethylation-modulated down-regulation of CDH1 expression contributes to the progression of esophageal cancer.

CDH1, a cell adhesion molecule, which plays a key role in maintaining the epithelial phenotype, is regarded as an invasion-suppressor gene in light of accumulating evidence from in vitro experiments and clinical observations. In an attempt to clarify the mechanism responsible for inactivation of this gene in carcinomas, we investigated the methylation status of the CDH1 gene 5'-CpG islands and its regulatory mechanism in the progression of esophageal squamous cell carcinoma. Real-time methylation-specific polymerase chain reaction (qMSP) and treatment with the demethylating agent 5-aza-2'-deoxycytidine (5-Aza-CdR) were conducted to analyze the methylation status at the CDH1 promoter region in the human esophageal carcinoma cell lines, EC1 and EC9706.