Fungal Endophthalmitis: Clinical Characteristics, Pathogens, and Factors Affecting Visual Outcome.

Aims: The aims of this study are to investigate the etiology, microbiological spectrum, and risk factors associated with visual outcomes of fungal endophthalmitis (FE) in a tertiary eye specialty hospital in Shanghai, China.

Methods: This was a retrospective, single-center case series. The clinical characteristics, etiology, microbiological spectrum, and management, as well as the visual outcomes, were analyzed.

[Study on rapid quality analysis method of Shengxuebao Mixture].

A rapid analysis method based on ultraviolet-visual(UV-Vis) spectroscopy, near infrared(NIR) spectroscopy and multivariable data analysis was established for quality evaluation of Shengxuebao Mixture. The contents of eight active ingredients of Shengxuebao Mixture including albiflorin, paeoniflorin, 2, 3, 5, 4'-tetra-hydroxy-stilbene-2-O-β-D-glucopyranoside, specnuezhenide,ecliptasaponin D, emodin, calycosin-7-glucoside and astragaloside Ⅳ were simultaneously detected by using this method. HPLC-UV-MS was used as a reference method for determining the contents of these ingredients.

[Quantitative analysis method of Shengxuebao Mixture by HPLC-UV-MS based on quality by design concept].

In this study, the HPLC-UV-MS method for the simultaneous determination of eight active ingredients of Shengxuebao Mixture were developed based on the concept of quality by design（QbD）with a stepwise optimization approach. After the analytical target profile（ATP）had been defined, albiflorin, paeoniflorin, 2, 3, 5, 4'-tetra-hydroxy-stilbene-2-O-β-D-glucopyranoside, specnuezhenide, ecliptasaponin D, emodin, calycosin-7-glucoside, and astragaloside Ⅳ were identified as the indicator components. The resolution and the signal-to-noise ratio of indicator components were then selected as critical method attributes (CMA) for the first step optimization.

Characterization of Vitreous and Aqueous Proteome in Humans With Proliferative Diabetic Retinopathy and Its Clinical Correlation.

Aims: Proliferative diabetic retinopathy (PDR) is associated with microvascular complications that cause biochemical changes in the human retina and alter the proteome of vitreous humor and aqueous humor (AH).

Methods: Human vitreous humor and AH of PDR subjects were collected. Subjects who had surgery for epiretinal membrane or macular hole served as controls.

Intraocular Pressure in Premature Low Birth Weight Infants.

Purpose: To evaluate the intraocular pressure (IOP) and central corneal thickness (CCT) in premature low birth weight (LBW) infants and their correlation with gestational age (GA).

Methods: IOP and CCT were measured in premature LBW infants (defined as a birth weight ≤ 1,500 g or birth GA ≤ 30 weeks) admitted to the neonatal intensive care unit at the University of Florida Division of Neonatology, UF Health Jacksonville.

Results: Ninety eyes of 45 premature LBW infants with mean birth GA of 28.

Erlotinib-associated severe bilateral recalcitrant keratouveitis after corneal EDTA chelation.

Purpose: We report a case of erlotinib-associated severe recalcitrant bilateral keratouveitis after uneventful corneal ethylenediaminetetraacetic acid (EDTA) chelation in a patient with non-small cell lung cancer (NSCLC); discontinuation of erlotinib led to complete resolution.

Observations: An elderly person presented with band keratopathy (BSK) of undetermined etiology in the both eyes, associated with foreign body sensation and constant tearing. The patient was on oral erlotinib treatment 150 mg PO daily for 1 year for NSCLC status post radiation therapy.

Diffuse lamellar keratitis after small-incision lenticule extraction.

Purpose: To determine the incidence and clinical features of diffuse lamellar keratitis (DLK) after small-incision lenticule extraction (SMILE).

Setting: Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China.

Design: Retrospective case control study.

No change in anterior chamber dimensions after femtosecond LASIK for hyperopia.

Objective: To compare the changes in anterior chamber volume (ACV), anterior chamber depth (ACD) and anterior chamber angle (ACA) before and 6 months after femtosecond laser in situ keratomileusis (LASIK) for hyperopia using the Pentacam HR device.

Methods: A total of 24 eyes of 24 consecutive hyperopic patients undergoing femtosecond LASIK were examined preoperatively and 6 months postoperatively. Anterior chamber volume; ACDs in the central, superior, inferior, nasal, and temporal quadrants; and ACA were measured using the Pentacam HR device.

Comparison of corneal sensation between small incision lenticule extraction (SMILE) and femtosecond laser-assisted LASIK for myopia.

Purpose: To compare the impact on corneal sensation after small incision lenticule extraction (SMILE) and femtosecond laser-assisted LASIK (femto-LASIK) in patients with myopia.

Methods: In this prospective, nonrandomized comparative study, 71 subjects were enrolled. Thirty-eight eyes of 38 patients underwent SMILE and 33 eyes of 33 patients underwent femto-LASIK.

Confocal comparison of corneal reinnervation after small incision lenticule extraction (SMILE) and femtosecond laser in situ keratomileusis (FS-LASIK).

Purpose: To evaluate corneal reinnervation, and the corresponding corneal sensitivity and keratocyte density after small incision lenticule extraction (SMILE) and femtosecond laser in situ keratomileusis (FS-LASIK).

Methods: In this prospective, non-randomized observational study, 18 patients (32 eyes) received SMILE surgery, and 22 patients (42 eyes) received FS-LASIK surgery to correct myopia. The corneal subbasal nerve density and microscopic morphological changes in corneal architecture were evaluated by confocal microscopy prior to surgery and at 1 week, 1 month, 3 months, and 6 months after surgery.

Small incision lenticule extraction (SMILE) and femtosecond laser LASIK: comparison of corneal wound healing and inflammation.

Aim: To evaluate and compare early corneal wound healing and inflammatory responses after small incision lenticule extraction (SMILE) versus femtosecond laser laser in situ keratomileusis (LASIK).

Methods: Thirty-six eyes of 36 rabbits underwent SMILE, while another 36 eyes of 36 rabbits were treated with femtosecond laser LASIK. All the eyes were subjected to the same refractive correction of -6.

The morphology of corneal cap and its relation to refractive outcomes in femtosecond laser small incision lenticule extraction (SMILE) with anterior segment optical coherence tomography observation.

Purpose: To investigate the morphology of corneal caps in femtosecond laser small incision lenticule extraction (SMILE) and its relation to the refractive outcomes.

Methods: A prospective study of fifty-four corneal caps created with VisuMax femtosecond laser were examined using an Fourier-domain optical coherence tomography at 1 day, 1 week, 1 month and 6 months after SMILE. The cap thickness at nine points on each of the four meridians (0°, 45°, 90°, 135°) and the diameter were measured.

Impact of pupil diameter on axial growth in orthokeratology.

Purpose: To compare axial elongation between myopic orthokeratology (OK) contact lens and spectacle wearers, and to investigate the impact of pupil diameter on axial growth in myopic children after OK treatment.

Methods: Fifty-two Chinese children aged 9 to 14 years were enrolled in this study, 27 for the OK group and 25 for the single vision spectacle lenses (SVL) group. Subjects in each group were further divided into two subcategories according to their baseline scotopic pupil diameters.

Evaluation of the repeatability of the Lenstar and comparison with two other non-contact biometric devices in myopes.

Background: The aim was to evaluate the repeatability of a new optical low-coherence reflectometric device Lenstar, as well as its agreement with the IOL Master and the Pentacam-HR system in myopes.

Methods: In a prospective study, 56 eyes of 28 myopes were examined with the Lenstar, the IOL Master and the Pentacam-HR system. The repeatability of the Lenstar was assessed.

Role of the Fas pathway in Pseudomonas aeruginosa keratitis.

Purpose: The role of the Fas pathway was tested in Pseudomonas aeruginosa-infected mouse cornea by contrasting the responses of FasL(-/-) and wild-type (WT) mice.

Methods: TUNEL staining, real-time RT-PCR, immunostaining, and ELISA assay were used.

Results: Compared with WT (resistant) mice, BALB/c FasL(-/-) exhibited significantly elevated bacterial counts and polymorphonuclear leukocyte numbers at 1 and 3 days postinfection (p.

Substance P delays apoptosis, enhancing keratitis after Pseudomonas aeruginosa infection.

Purpose: Apoptosis was examined after Pseudomonas aeruginosa corneal infection in C57BL/6 (B6, susceptible) and BALB/c (resistant) mice.

Methods: TUNEL staining, real-time RT-PCR, polymorphonuclear neutrophils (PMNs) and macrophage (Mphi) depletion, and immunostaining were used.

Results: Intense TUNEL staining was seen in BALB/c versus B6 cornea at 1 versus 3 days after infection (PI) and correlated with mRNA levels for caspase-3.