Publications by authors named "Zilei Huang"

Antimicrobial resistance poses a significant global challenge, demanding innovative approaches, such as the CRISPR-Cas-mediated resistance plasmid or gene-curing system, to effectively combat this urgent crisis. To enable successful curing of antimicrobial genes or plasmids through CRISPR-Cas technology, the development of an efficient broad-host-range delivery system is paramount. In this study, we have successfully designed and constructed a novel functional gene delivery plasmid, pQ-mini, utilizing the backbone of a broad-host-range Inc.

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Article Synopsis
  • Salmonella is a type of bacteria found in livestock that can make people sick, especially when food gets contaminated.
  • The study looked at how a specific Salmonella strain became resistant to multiple drugs and learned that a tiny change in its genes helped it survive better in animals without making them sick.
  • This change also made it tougher against things like heat and cold, meaning it could stick around in the environment longer and spread to other animals and people, which could lead to more sickness.
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Epothilones are a kind of macrolides with strong cytotoxicity toward cancer cells and relatively lower side effects compared with taxol. Epothilone B derivate ixabepilone has been used for the clinical treatment of advanced breast cancer. However, the low yield of epothilones and the difficulty in the genetic manipulation of limited their wider application.

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Gliotoxin is an important epipolythiodioxopiperazine, which was biosynthesized by the gene cluster in . However, the regulatory mechanism of gliotoxin biosynthesis remains unclear. In this study, a novel Zn2Cys6 transcription factor DcGliZ that is responsible for the regulation of gliotoxin biosynthesis from the deep-sea-derived fungus was identified.

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Lithocarols A-F (1-6) possessing novel highly-oxygenated isobenzofuran core, together with a related known compound isoprenylisobenzofuran A (7) were isolated from the marine-derived fungus Phomopsis lithocarpus FS508. Among them, lithocarols A-E (1-5) represent the first examples of poly-ketal derivatives in tenellone family. The structures for all these compounds were fully elucidated by spectroscopic analysis, X-ray diffraction, and electronic circular dichroism calculations.

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Five new benzophenone derivatives named tenellones D⁻H (⁻), sharing a rare naturally occurring aldehyde functionality in this family, and a new eremophilane derivative named lithocarin A (), together with two known compounds ( and ), were isolated from the deep marine sediment-derived fungus FS508. All of the structures for these new compounds were fully characterized and established on the basis of extensive spectroscopic interpretation and X-ray crystallographic analysis. Compound exhibited cytotoxic activity against HepG-2 and A549 cell lines with IC values of 16.

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Agarwood is a precious traditional Chinese medicine with a variety of pharmacological effects. Although efforts have been made in elucidating the mechanism of agarwood formation, little progress is obtained till now. Therefore, the molecular mechanism of agarwood formation needs to be further explored using different biological approaches.

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Gliotoxin, produced by fungi, is an epipolythiodioxopiperazine (ETP) toxin with bioactivities such as anti-liver fibrosis, antitumor, antifungus, antivirus, antioxidation, and immunoregulation. Recently, cytotoxic gliotoxins were isolated from a deep-sea-derived fungus, . However, the biosynthetic pathway for gliotoxins in remains unclear.

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is a plant pathogenic fungus that infects different crops and decreases the yield of economical crops, including soybean, cotton, corn, pepper, and tomato. Until now, the pathogenic mechanism of has remained unclear. Different types of trichothecene mycotoxins were isolated from , and trichothecene was considered as a plant pathogenic factor of .

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Bioethanol is becoming increasingly important in energy supply and economic development. However, the low yield of bioethanol and the insufficiency of high-efficient genetic manipulation approaches limit its application. In this study, a novel transcription activator-like effector nuclease (TALEN) vector containing the left and right arms of TALEN was electroporated into Saccharomyces cerevisiae strain As2.

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