Vacuolar H-ATPase determines daughter cell fates through asymmetric segregation of the nucleosome remodeling and deacetylase complex.

Asymmetric cell divisions (ACDs) generate two daughter cells with identical genetic information but distinct cell fates through epigenetic mechanisms. However, the process of partitioning different epigenetic information into daughter cells remains unclear. Here, we demonstrate that the nucleosome remodeling and deacetylase (NuRD) complex is asymmetrically segregated into the surviving daughter cell rather than the apoptotic one during ACDs in .

Transposase-assisted tagmentation: an economical and scalable strategy for single-worm whole-genome sequencing.

AlphaMissense identifies 23 million human missense variants as likely pathogenic, but only 0.1% have been clinically classified. To experimentally validate these predictions, chemical mutagenesis presents a rapid, cost-effective method to produce billions of mutations in model organisms.

Highly diverse sputum microbiota correlates with the disease severity in patients with community-acquired pneumonia: a longitudinal cohort study.

Background: Community-acquired pneumonia (CAP) is a common and serious condition that can be caused by a variety of pathogens. However, much remains unknown about how these pathogens interact with the lower respiratory commensals, and whether any correlation exists between the dysbiosis of the lower respiratory microbiota and disease severity and prognosis.

Methods: We conducted a retrospective cohort study to investigate the composition and dynamics of sputum microbiota in patients diagnosed with CAP.

A Lateral Flow-Recombinase Polymerase Amplification Method for Detection.

The greater yam (), a widely cultivated and nutritious food crop, suffers from widespread yield reduction due to anthracnose caused by . Latent infection often occurs before anthracnose phenotypes can be detected, making early prevention difficult and causing significant harm to agricultural production. Through comparative genomic analysis of 60 genomes of 38 species from the genus, this study identified 17 orthologous gene groups (orthogroups) that were shared by all investigated strains but absent from all other species.

Correction: Zhang et al. The Stressing State Features of a Bottom Frame Structure Revealed from the Shaking Table Strain Data. 2023, , 1809.

In the original publication [...

Inhibition of the chromatin remodeling factor NURF rescued sterility by a clinic variant of NuRD.

The nucleosome remodeling and deacetylase (NuRD) complex is essential for gene expression and cell fate determination, and missense mutations of NuRD caused neurodevelopmental diseases. However, the molecular pathogenesis of clinic NuRD variants is unknown. Here, we introduced a clinic CHD3 (L915F) variant into homologue LET-418, impairing germline and vulva development and ultimately causing animal sterility.

The Stressing State Features of a Bottom Frame Structure Revealed from the Shaking Table Strain Data.

As a classic issue, structural seismic bearing capacity could not be accurately predicted since it was based on a structural ultimate state with inherent uncertainty. This result led to rare research efforts to discover structures' general and definite working laws from their experimental data. This study is to reveal the seismic working law of a bottom frame structure from its shaking table strain data by applying structural stressing state theory: (1) The tested strains are transformed into generalized strain energy density (GSED) values.

Characterizing Carbon Emissions and the Associations with Socio-Economic Development in Chinese Cities.

Reducing carbon emissions in cities is crucial for addressing climate change, while the city-level emissions of different compositions and their relationships with socio-economic features remain largely unknown in China. Here, we explored the city-level emission pattern from the industrial, transportation, and household sectors and the emission intensity, as well as their associations with socio-economic features in China, using the up-to-date (2020) CO emissions based on 0.1° grid (10 × 10 km) emission data.

Research on the General Failure Law of a CTRC Column by Modeling FEM Output Data.

In this paper, a finite element model (FEM) is developed based on a set of circular steel tube reinforced concrete (CTRC) columns with axial compression and eccentric compression tests. The stressing state characteristics of the FEM are modeled in the form of characteristic pairs (mode-characteristic parameters) based on the structural stressing state theory and the proposed correlation modeling method. The slope increasing criterion is applied to the correlation characteristic parameter curve to obtain the characteristic point Q where the CTRC stressing state undergoes a qualitative change, and the characteristic point Q is defined as the new failure load point of the CTRC column.

Dynamics of the Upper Respiratory Tract Microbiota and Its Association with Mortality in COVID-19.

Alteration of human respiratory microbiota had been observed in coronavirus disease (COVID-19). How the microbiota is associated with the prognosis in COVID-19 is unclear. To characterize the feature and dynamics of the respiratory microbiota and its associations with clinical features in patients with COVID-19.

RNA-Associated Co-expression Network Identifies Novel Biomarkers for Digestive System Cancer.

Cancers of the digestive system are malignant diseases. Our study focused on colon cancer, esophageal cancer (ESCC), rectal cancer, gastric cancer (GC), and rectosigmoid junction cancer to identify possible biomarkers for these diseases. The transcriptome data were downloaded from the TCGA database (The Cancer Genome Atlas Program), and a network was constructed using the WGCNA algorithm.

Regulator Network Analysis of Rice and Maize Yield-Related Genes.

Rice and maize are the principal food crop species worldwide. The mechanism of gene regulation for the yield of rice and maize is still the research focus at present. Seed size, weight and shape are important traits of crop yield in rice and maize.

Dynamics of severe acute respiratory syndrome coronavirus 2 genome variants in the feces during convalescence.

In response to the current coronavirus disease 2019 (COVID-19) pandemic, it is crucial to understand the origin, transmission, and evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which relies on close surveillance of genomic diversity in clinical samples. Although the mutation at the population level had been extensively investigated, how the mutations evolve at the individual level is largely unknown. Eighteen time-series fecal samples were collected from nine patients with COVID-19 during the convalescent phase.

Genome-Wide Analysis of LysM-Containing Gene Family in Wheat: Structural and Phylogenetic Analysis during Development and Defense.

The lysin motif (LysM) family comprise a number of defense proteins that play important roles in plant immunity. The LysM family includes LysM-containing receptor-like proteins (LYP) and LysM-containing receptor-like kinase (LYK). LysM generally recognizes the chitin and peptidoglycan derived from bacteria and fungi.

Basic polar and hydrophobic properties are the main characteristics that affect the binding of transcription factors to methylation sites.

Motivation: Methylation and transcription factors (TFs) are part of the mechanisms regulating gene expression. However, the numerous mechanisms regulating the interactions between methylation and TFs remain unknown. We employ machine-learning techniques to discover the characteristics of TFs that bind to methylation sites.

Genomic Diversity of Severe Acute Respiratory Syndrome-Coronavirus 2 in Patients With Coronavirus Disease 2019.

Background: A novel coronavirus (CoV), severe acute respiratory syndrome (SARS)-CoV-2, has infected >75 000 individuals and spread to >20 countries. It is still unclear how fast the virus evolved and how it interacts with other microorganisms in the lung.

Methods: We have conducted metatranscriptome sequencing for bronchoalveolar lavage fluid samples from 8 patients with SARS-CoV-2, and also analyzed data from 25 patients with community-acquired pneumonia (CAP), and 20 healthy controls for comparison.

An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice.

Brown planthopper (BPH) is one of the most destructive insects affecting rice ( L.) production. Phenylalanine ammonia-lyase (PAL) is a key enzyme involved in plant defense against pathogens, but the role of PAL in insect resistance is still poorly understood.

Transcription factors-DNA interactions in rice: identification and verification.

The completion of the rice genome sequence paved the way for rice functional genomics research. Additionally, the functional characterization of transcription factors is currently a popular and crucial objective among researchers. Transcription factors are one of the groups of proteins that bind to either enhancer or promoter regions of genes to regulate expression.