Iron promotes both ferroptosis and necroptosis in the early stage of reperfusion in ischemic stroke.

Programmed cell death contributes to neurological damage in ischemic stroke, especially during the reperfusion stage. Several cell death pathways have been tested preclinically and clinically, including ferroptosis, necroptosis, and apoptosis. However, the sequence and complex interplay between cell death pathways during ischemia/reperfusion remains under investigation.

Ambient-pressure alkoxycarbonylation for sustainable synthesis of ester.

Alkoxycarbonylation reactions are common in the chemical industry, yet process sustainability is limited by the inefficient utilization of CO. In this study, we address this issue and demonstrate that significant improvements can be achieved by adopting a heterogeneously catalyzed process, using a Ru/NbO catalyst. The Ru/NbO catalyst enables the direct synthesis of methyl propionate, a key industrial commodity, with over 98% selectivity from CO, ethylene and methanol, without any ligands or acid/base promoters.

Atomically dispersed cobalt catalysts for tandem synthesis of primary benzylamines from oxidized β-O-4 segments.

This work presents an innovative approach focusing on fine-tuning the coordination environment of atomically dispersed cobalt catalysts for tandem synthesis of primary benzylamines from oxidized lignin model compounds. By meticulously regulating the Co-N coordination environment, the activity of these catalysts in the hydrogenolysis and reductive amination reactions was effectively controlled. Notably, our study demonstrates that, in contrast to cobalt nanoparticle catalysts, atomically dispersed cobalt catalysts exhibit precise control of the sequence of hydrogenolysis and reductive amination reactions.

Innovative tumor interstitial fluid-triggered carbon dot-docetaxel nanoassemblies for targeted drug delivery and imaging of HER2-positive breast cancer.

In this study, we have developed an innovative pH-triggered nanomedicine delivery system, targeting HER2-positive breast cancer cells for effective low-cost, imaging-guided drug delivery and precise therapy. The key feature of this system lies in its unique tumor interstitial fluid microenvironment-responsive drug release behavior which achieved tumor site-specific drug delivery. Our in vitro experiments demonstrated that the carbon dot-integrated material achieves more efficient DTX release (96.

First record of theridiosomatid genus Suzuki, Hiramatsu & Tatsuta, 2022 from Anhui Province, China, with the description of a new species (Araneae, Theridiosomatidiae).

Background: Only two species are known from the world, (Zhu, Zhang & Chen, 2001) from China and Suzuki, Hiramatsu & Tatsuta, 2022 from Ryukyu Islands. No other species have been recorded from other locations.

New Information: A new species, is described from Anhui Province, China.

Using tungsten oxide quantum-dot enhanced electrochemiluminescence to measure thrombin activity and screen its inhibitors.

A thrombin-activity-based electrochemiluminescence (ECL) biosensor was successfully constructed using tungsten oxide quantum dots (WO QD) as the co-reactant and thrombin-cleavable peptides as the recognizer. Specifically, Ru(bpy) were doped on silica nanoparticles (Ru@SiO), which greatly enhanced the ECL potential. AuNPs@WO QDs composite was then prepared to accelerate electron transfer and improve the ECL signal by 219 times.

MXene-sensitized electrochemiluminescence sensor for thrombin activity detection and inhibitor screening.

Thrombin, a crucial enzyme involved in blood coagulation and associated diseases, requires accurate detection of its activity and screening of inhibitors for clinical diagnosis and drug discovery. To address this, an electrochemiluminescence (ECL) method was developed to detect thrombin activity based on the sensitization of TiCT MXene, which could sensitize the Ru(bpy) ECL system greatly. The thrombin-cleavable substrate bio-S-G-R-P-V-L-G-C was used as recognizer to evaluate the activity of thrombin.

The complete chloroplast genome sequence of (Crassulaceae).

is a traditional medicinal plant in China, with few reports on its chloroplast genome. In this study, the chloroplast genome of was characterized, and its phylogenetic position among other closely related species was studied. The results showed that the full length of the chloroplast genome was 150,074 bp, containing a large single-copy (LSC) region and a small single-copy (SSC) region of 81,730 and 16,726 bp, respectively, as well as two inverted repeat regions (IRs) of 25,809 bp like other plants.

Whole genome sequencing reveals novel genetic mutations of Helicobacter pylori associating with resistance to clarithromycin and levofloxacin.

Background: Detection of mutations in one or a couple of genes may not provide enough data or cover all the genomic DNA variance related to antibiotic resistance of Helicobacter pylori to clarithromycin (CLA) and levofloxacin (LVX). We aimed to perform whole genome sequencing to explore novel antibiotic resistance-related genes to increase predictive accuracy for future targeted sequencing tests.

Methods: Gastric mucosal biopsies were taken during upper endoscopy in 27 H.

Rapid screening and identification of anticoagulation component from carthami flos by two-dimensional thrombin affinity chromatography combined with HPLC-MS/MS.

Carthami flos, commonly known as Honghua in China, is the dried floret of safflower and widely acknowledged as a blood stasis promoting herb. The study aimed at investigating the relationship between thrombin and carthami flos through a high-performance thrombin affinity chromatography combined with a high-performance liquid chromatography-tandem mass spectrometry system. First, thrombin was immobilized on the glutaraldehyde-modified amino silica gel to prepare the thrombin affinity stationary phase, which was packed into a small column (1.

Feedback from artificial intelligence improved the learning of junior endoscopists on histology prediction of gastric lesions.

Artificial intelligence (AI)-assisted image classification has been shown to have high accuracy on endoscopic diagnosis. We evaluated the potential effects of use of an AI-assisted image classifier on training of junior endoscopists for histological prediction of gastric lesions. An AI image classifier was built on a convolutional neural network with five convolutional layers and three fully connected layers A Resnet backbone was trained by 2,000 non-magnified endoscopic gastric images.

5-Lipoxagenase deficiency attenuates L-NAME-induced hypertension and vascular remodeling.

Background: Abnormalities of the L-arginine-nitric oxide pathway induce hypertension. 5-Lipoxygenase (5-LO) is the key enzyme involved in synthesis of leukotrienes (LTs). However, whether nitricoxide synthase dysfunction induces hypertensive vascular remodeling by regulating 5-LO activity and its downstream inflammatory metabolites remains unknown.

[Optimization of the matrix formulation of compound Die Da Zhen Tong cataplasm by uniform design].

Objective: To optimize the matrix formulation of compound Die Da Zhen Tong cataplasm.

Methods: The optimal preparation was selected by U17 (17(16)) uniform design, independent variables were the percentage ratio of the matrix formulation component part in compound Die Da Zhen Tong cataplasm,and the viscosity, continued viscosity and overall desirability used as indexes were dependent variables.

Results: The percentage of the matrix formulation component part in compound Die Da Zhen Tong cataplasm was, NP-700: carbomer 980: PVP K-90: dihydroxy aluminum: tartaric: kaolinite: sorbitol: glycerin = 5: 1.