Four-Dimensional Data-Independent Acquisition-Based Proteomic Profiling Combined with Transcriptomic Analysis Reveals the Involvement of the Slym1-SlFHY3-CAB3C Module in Regulating Tomato Leaf Color.

In green plants, the chloroplast is responsible for light energy transition and organic assimilation. However, the molecular mechanisms underlying chloroplast development in horticultural crops remain unclear. Here, four-dimensional data-independent acquisition-based proteomic profiling identified 1,727 differentially expressed proteins between "Zhongshu 4" (ZS4) and () leaves, a considerable proportion of which were down-regulated chloroplast proteins.

Ultrasensitive Room Temperature Formaldehyde Sensors Based on F Doped ZnO Nanostructures Activated by UV Light.

It is urgent to develop an ultrasensitive formaldehyde (HCHO) sensor that can operate at room temperature and has a low detection limit. Metal oxide semiconductors are excellent gas sensitive materials. Therefore, in this paper, we present the synthesis of fluorine (F) doped zinc oxide (ZnO) porous nanomaterials through a straightforward one-pot method with the optimization of F doping levels to achieve the detection of low concentrations of HCHO under UV light at room temperature.

Interactions, structures, and functions of pomegranate peel reinforced starch film.

This work aimed to exploit the effects of pomegranate peel (PP) addition (10 %) and homogenization process (0-12,000 rpm) on the film-forming behavior and interactions of starch film to improve its microstructures and properties. Microrheology results showed that the viscosity of the film suspension was reduced by homogenization, and the amylopectin aggregation was restricted by PP particles under film formation. This helped improve the accessibility of starch and PP, increasing the hydrogen bond energy in the composites from 18.

Overexpression of OsGASR1 promotes Al tolerance in rice.

Aluminum (Al) toxicity in acid soils poses a significant threat to rice, which exhibits highly complex genetic mechanisms for both external detoxification and internal tolerance among cereal crops. Although several genes involved Al tolerance have been identified, the molecular mechanisms underlying Al tolerance in rice remain to be fully explored. Here, we functionally characterized the gibberellin-stimulated transcription gene OsGASR1, which encodes a small cysteine-rich peptide localized to the nucleus and cytoplasm and plays a significant role in Al tolerance in rice.

Secondary Metabolites of : Chemical Structures, Biological Activity and Application Prospects.

, as a macrofungus with both medicinal and dietary applications, is renowned for its rich content of bioactive substances. The recent advancements in research have significantly enhanced our understanding of its polysaccharides, cellulose-degrading enzymes, and wide range of secondary metabolites. This paper provides a comprehensive review of the artificial cultivation techniques and the chemical profiling of over 100 secondary metabolites identified in , including terpenoids, phenols, and various other classes.

Bioinspired multifunctional cellulose film: In situ bacterial capturing and killing for managing infected wounds.

Bacterial infection of cutaneous wounds can easily lead to occurrence of chronic wounds and even more serious diseases. Therefore, multifunctional, biodegradable, and reusable wound dressings that can quickly manage wound infection and promote wound healing are urgently desired. Herein, inspired by the "capturing and killing" action of Thunb.

Anchoring Pt single atoms on specific nitrogen vacancies of carbon nitride to accelerate photogenerated carrier transfer.

The anchoring sites of metal single atoms are closely related to photogenerated carrier dynamics and surface reactions. Achieving smooth photogenerated charge transfer through precise design of single-atom anchoring sites is an effective strategy to enhance the activity of photocatalytic hydrogen evolution. In this study, Pt single atoms were loaded onto ultra-thin carbon nitride with two-coordination nitrogen vacancies (V-UCN-Pt) and ultra-thin carbon nitride with three-coordination nitrogen vacancies (V-UCN-Pt).

Therapeutic potential of salidroside in type I diabetic erectile dysfunction: Attenuation of oxidative stress and apoptosis via the Nrf2/HO-1 pathway.

The primary objective of this work was to delve into the potential therapeutic advantages and dissect the molecular mechanisms of salidroside in enhancing erectile function in rats afflicted with diabetic microvascular erectile dysfunction (DMED), addressing both the whole-animal and cellular dimensions.We established a DMED model in Sprague‒Dawley (SD) rats and conducted in vivo experiments. The DMED rats were administered varying doses of salidroside, the effects of which on DMED were compared.

Design, Synthesis, and Biological Evaluation of 1,2,4-Oxadiazole Derivatives Containing an Aryl Carboxylic Acid Moiety as Potent Sarbecovirus Papain-like Protease Inhibitors.

Papain-like protease (PLpro) is a promising therapeutic target for its pivotal role in the life cycle of SARS-CoV-2. A series of 1,2,4-oxadiazole derivatives was designed and synthesized via a ring formation strategy based on SARS-CoV-2 PLpro-GRL0617 complex structure. Systematic structure-activity relationship studies revealed that introducing oxadiazole and aryl carboxylic acid moieties to GRL0617 enhanced the enzymatic inhibition activity, affinity, and deubiquitination capacity toward PLpro.

Electrochemically Dealloying Engineering toward Integrated Monolithic Electrodes with Superior Electrochemical Li-Storage Properties.

Integrated monolithic electrodes (IMEs) free of inactive components demonstrate great potential in boosting energy-power densities and cycling life of lithium-ion batteries. However, their practical applications are significantly limited by low active substance loading (< 4.0 mg cm and 1.

Recent Advances of Food Hazard Detection Based on Artificial Nanochannel Sensors.

Food quality and safety are related to the health and safety of people, and food hazards are important influencing factors affecting food safety. It is strongly necessary to develop food safety rapid detection technology to ensure food safety. As a new detection technology, artificial nanochannel-based electrochemical and other methods have the advantages of being real-time, simple, and sensitive and are widely used in the detection of food hazards.

Visible Light-Gating Responsive Nanochannel for Controlled Release of the Fungicide.

Fungicides have been widely used to protect crops from the disease of pythium aphanidermatum (PA). However, excessive use of synthetic fungicides can lead to fungal pathogens developing microbicide resistance. Recently, biomimetic nano-delivery systems have been used for controlled release, reducing the overuse of fungicides, and thereby protecting the environment.

Brain Immune Cell Infiltration and Serum Metabolomic Characteristics Reveal that Lauric Acid Promotes Immune Cell Infiltration in Brain and Streptococcus suis Meningitis in Mice.

Although naturally Streptococcus suis serotype 2 (SS2) causes meningitis resulting in death or sequela of neurological symptoms in pigs and humans, severely threatening public health in the world, it has been difficult to build up and confirm experimental meningitis mouse models with obvious neurological syndrome for about two decades, which strongly hampers the in-depth study on the control measures and mechanisms of SS2-induced meningitis. In this study, a typical meningitis mouse model of SS2 was successfully established, as confirmed by the behavioral indicators of balance beam test, suspension test, and gait analysis. With bacteria gathering in the brain, distinguishable unique features including meningeal thickening, vacuolization of the Nissl body, brain barrier damage, glial cell activation, and more infiltration of T cells, macrophages, and DCs are observed in SS2 meningitis mice with typical neurological signs.

Wearable-Sensor-Based Weakly Supervised Parkinson's Disease Assessment with Data Augmentation.

Parkinson's disease (PD) is the second most prevalent dementia in the world. Wearable technology has been useful in the computer-aided diagnosis and long-term monitoring of PD in recent years. The fundamental issue remains how to assess the severity of PD using wearable devices in an efficient and accurate manner.

Exploring pollutant joint effects in disease through interpretable machine learning.

Identifying the impact of pollutants on diseases is crucial. However, assessing the health risks posed by the interplay of multiple pollutants is challenging. This study introduces the concept of Pollutants Outcome Disease, integrating multidisciplinary knowledge and employing explainable artificial intelligence (AI) to explore the joint effects of industrial pollutants on diseases.

LC-MS/MS analysis of Shenghui decoction component and its effect on learning and memory and neuroprotection in sleep deprivation model mice.

Background: In recent years, sleep problems have emerged as a significant factor in the development of diseases that influence cognitive function. The inflammatory response may have a role in the neurobiological processes of sleep deprivation, resulting in impairment of memory and learning. Shenghui Decoction (SHD) is a classic formula in Chinese medicine used to treat forgetfulness and insomnia.

Development and application of an indirect ELISA and nested PCR for the epidemiological analysis of among pigs in China.

Introduction: () is an important opportunistic and zoonotic pathogen which is associated with many diseases in humans and animals. However, the pathogenicity of has been neglected and the prevalence of is poorly studied due to the lack of rapid and sensitive diagnosis techniques.

Methods: In this study, we infected mice and pigs with strain from a human patient.

Neutrophil extracellular traps in adult diseases and neonatal bacterial infectious diseases: A review.

Neutrophils, the most abundant type of white blood cells, are pivotal in fighting bacterial infections due to their immunological and anti-infection capabilities. In recent years, scientists have discovered a novel mechanism known as neutrophil extracellular traps, which are fibrous networks primarily released by neutrophils that combat bacterial infections. There is a growing interest in studying NETs and their role in human infectious diseases, particularly in neonates susceptible to bacterial infections.

Development and characterization of an active starch-based film as a chlorogenic acid delivery system.

Given its health benefits for the human body, chlorogenic acid (CA) offers promising applications in the food industry. However, the instability and low bioavailability of CA remain to be solved. In this paper, a starch-based film prepared by the homogenization and solution-casting method was used as an effective carrier to alleviate these problems.

Towards sustainability: An integrated life cycle environmental-economic insight into cow manure management.

Waste management signifies an equilibrium between environmental and economic factors. However, a comprehensive understanding of the integrated life cycle environmental-economic performance of waste management activities remains unclear. To facilitate a systematic linkage between the economic and environmental sectors, a regionalized life cycle assessment-based life cycle costing method was developed based on China's actual status quo.

Fine Mapping and Identification of a Candidate Gene for the Glossy Green Trait in Cabbage ( var. ).

In higher plants, cuticular wax deposited on the surface of epidermal cells plays an important role in protecting the plant from biotic and abiotic stresses; however, the molecular mechanism of cuticular wax production is not completely understood. In this study, we identified a glossy green mutant (98-1030gl) from the glaucous cabbage inbred line 98-1030. Scanning electron microscopy indicated that the amount of leaf cuticular wax significantly decreased in 98-1030gl.

Uncovering the energy-carbon-water footprint of waste rubber recycling: Integrated environmental and economic perspectives.

The commitment to waste management has gained increasing momentum as global waste generation continues to skyrocket and threaten the environment. However, detailed assessments and clear insights remain absent to address the global waste utilization conundrum. This study evaluated the impact-oriented energy, carbon, and water (ECW) footprints of three typical scenarios for a waste recycling activity (i.

Cartilage-inspired terpolymer hydrogel with excellent mechanical properties and superior lubricating ability.

Osteoarthritis (OA), the most common degenerative joint disorder, seriously affects patients' daily activities. Recently, hydrogels, due to their similar structure to articular cartilage, have shown great potential as cartilage-repairing materials. In the present work, we developed a simple process for fabricating terpolymer [P(acrylamide--acrylic acid--2-acrylamido-2-methyl-1-propanesulfonic acid)/Fe] hydrogel [P(AAm--AAc--AMPS)/Fe].

Identification of the SARS-unique domain of SARS-CoV-2 as an antiviral target.

SARS-CoV-2 nsp3 is essential for viral replication and host responses. The SARS-unique domain (SUD) of nsp3 exerts its function through binding to viral and host proteins and RNAs. Herein, we show that SARS-CoV-2 SUD is highly flexible in solution.

Genome-Wide Identification of the U-Box E3 Ubiquitin Ligase Gene Family in Cabbage ( var. ) and Its Expression Analysis in Response to Cold Stress and Pathogen Infection.

Plant U-box E3 ubiquitin ligases (PUBs) play an important role in growth, development, and stress responses in many species. However, the characteristics of U-box E3 ubiquitin ligase genes in cabbage ( var. ) are still unclear.