Photo-Crosslinking Hydrogel Based on Porcine Small Intestinal Submucosa Decellularized Matrix/Fish Collagen/GelMA for Culturing Small Intestinal Organoids and Repairing Intestinal Defects.

Organoid technology, as an innovative approach in biomedicine, exhibits promising prospects in disease modeling, pharmaceutical screening, regenerative medicine, and oncology research. However, the use of tumor-derived Matrigel as the primary method for culturing organoids has significantly impeded the clinical translation of organoid technology due to concerns about potential risks, batch-to-batch instability, and high costs. To address these challenges, this study innovatively introduced a photo-crosslinkable hydrogel made from a porcine small intestinal submucosa decellularized matrix (SIS), fish collagen (FC), and methacrylate gelatin (GelMA).

Exploring the effect of carbon nanoparticle tracing technique on five-year overall survival and disease-free survival in patients undergoing radical surgery for colorectal cancer: a retrospective study.

Background: To investigate the effect of preoperative carbon nanoparticle tracing technique via colonoscopy on the five-year overall survival and disease-free survival rates in patients undergoing radical resection for colorectal cancer.

Methods: A retrospective cohort study was conducted to collect data from patients diagnosed with colorectal cancer who underwent radical resection with complete postoperative pathological information at the First Affiliated Hospital of Chongqing Medical University from March 2013 to February 2017. Patients with multiple primary cancers were excluded, resulting in 2,237 eligible patients in the study.

Analysis of factors affecting microbial degradation of cyanobacterial toxins based on theoretical calculations.

Cyanobacterial toxins are the most common algal toxins, which are highly toxic and can persist in the aquatic environment without easy degradation, posing risks to the ecosystem and human health that cannot be ignored. Although microbiological methods for the removal of cyanobacterial toxins from aqueous environments are highly efficient, their degradation efficiency is susceptible to many abiotic environmental factors. In this paper, Microcystin-LR (MC-LR) and its microbial degrading enzymes were selected to study the effects of common environmental factors (temperature (T), NO, NH, Cu, Zn) and their levels during microbial degradation of cyanobacterial toxins in aqueous environments by using molecular docking, molecular dynamics simulation, analytical factor design, and the combined toxicokinetics of TOPKAT (toxicity prediction).

Risk for Donor-Derived Syphilis after Kidney Transplantation, China, 2007-2022.

To evaluate the risk of acquiring syphilis from a donated kidney, we evaluated kidney transplantation pairs from West China Hospital, Sichuan, China, during 2007-2022. Donor-derived syphilis was rare. Risk may be higher if donors have active syphilis and may be reduced if recipients receive ceftriaxone.

Tracing the impacts of ecological water replenishment on the sources and transformation of groundwater nitrate through isotope and microbial analysis.

Ecological water replenishment (EWR) changes the recharge conditions, flow fields, and physicochemical properties of regional groundwater. However, the resulting impacts on mechanisms regulating the sources and transformation of groundwater nitrate remain unclear. This study investigated how EWR influences the sources and transformation processes of groundwater nitrate using an integrated approach of Water chemistry analysis and stable isotopes (δN-NO and δO-NO) along with microbial techniques.

A new strategy for groundwater level prediction using a hybrid deep learning model under Ecological Water Replenishment.

Accurate prediction of the groundwater level (GWL) is crucial for sustainable groundwater resource management. Ecological water replenishment (EWR) involves artificially diverting water to replenish the ecological flow and water resources of both surface water and groundwater within the basin. However, fluctuations in GWLs during the EWR process exhibit high nonlinearity and complexity in their time series, making it challenging for single data-driven models to predict the trend of groundwater level changes under the backdrop of EWR.

Enantioselective Synthesis of Diarylmethylamines through the Aza-Friedel-Crafts Reaction of 1,3,5-Trialkoxy Benzenes and -Sulfonyl Aldimines Catalyzed by BINOL-Derived Disulfonimides.

A 1,1'-bi-2-naphthol (BINOL)-derived disulfonimide (DSI)-catalyzed enantioselective aza-Friedel-Crafts reaction between 1,3,5-trialkoxy benzenes and -sulfonyl aldimines gives direct access to a series of chiral diarylmethylamines in good yields and good to excellent enantioselectivities (up to 97% ee). This reaction provides a useful protocol for the direct synthesis of diarylmethylamine derivatives.

Environmental risk of tailings pond leachate pollution: Traceable strategy for leakage channel and influence range of leachate.

Identifying the leakage channel and the influencing range is essential for controlling the environmental risks of leachate from the tailings pond. The investigation of leachate pollution in tailings pond has the defect of focusing only on the scope of tailings pond in recent studies. This study innovatively built a comprehensive investigation and accurate verification system for leachate leakage of tailings pond integrated with the aeromagnetic survey, ground penetrating radar, hydrochemistry and isotope coupling methods.

Impact of extreme temperatures on the performance evaluation of China's work-related injury insurance system.

With the influence of climate change resulting in more extreme days, a rise in the number of work-related injuries could be expected. The literature has addressed the performance evaluation of a work-related injury insurance (WII) system via a two-stage structure with input/output correlation as well as the impact of extreme temperatures under different scenarios. This article thus evaluates the performance of a system comprised of operational and service sub-systems under three scenarios of extreme temperatures and proposes a hybrid two-stage dynamic data envelopment analysis (DEA) model with nondiscretionary variables for measuring integrated WII efficiency under the three scenarios.

Chaihujialonggumulitang shows psycho-cardiology therapeutic effect on acute myocardial infarction with comorbid anxiety by the activation of Nrf2/HO-1 pathway and suppression of oxidative stress and apoptosis.

Background: Anxiety is a common comorbidity of cardiovascular diseases, which deteriorated cardiac function. Chaihujialonggumulitang (BFG) was reported to have antioxidant properties, alleviate myocardial ischemia injury and improve anxiety-like behavior. The Nuclear factor erythroid 2-related factor 2 (Nrf2) /heme oxygenase-1 (HO-1) pathway is the main mechanism to defend against oxidative stress, and improve cardiac function.

Enantioselective synthesis of 2-indolyl methanamine derivatives through disulfonimide-catalyzed Friedel-Crafts C2-alkylation of 3-substituted indoles with imines.

An asymmetric Friedel-Crafts C2-alkylation between 3-substituted indoles and imines catalyzed by chiral BINOL-derived disulfonimides (DSIs) has been developed. This reaction tolerated a wide range of 3-substituted indoles and imines, affording a series of chiral 2-indolyl methanamine derivatives in good yields with good to excellent enantioselectivities (up to 98% ee). This is a useful protocol for the direct synthesis of 2-indolyl methanamine derivatives.

Machine Retrograde Perfusion of Deceased Donor Kidneys: A Prospective Study.

To maximize the utilization of potential kidneys, improving perfusion and preservation techniques is necessary. We investigated the safety and efficacy of retrograde machine perfusion of kidneys from deceased donors. A total of 30 kidneys were included and all the grafts were preserved in the Kidney Transporter machines.

miR-17-5p attenuates kidney ischemia-reperfusion injury by inhibiting the PTEN and BIM pathways.

Background: Kidney ischemia-reperfusion (I/R) injury is an independent risk factor for delayed graft function after kidney transplantation with long-term graft survival deterioration. Previously, we found that the upregulated expression of miR-17-5p exerts a protective effect in kidney I/R injury, but the mechanism has not been clearly studied.

Methods: A kidney I/R injury model was induced in adult C57BL/6 male mice (20-22 g) by clamping both kidney pedicles for 30 min.

A Nomogram Model to Predict Prognosis of Patients With Genitourinary Sarcoma.

Objectives: The aim of this study is to evaluate the significant factors influencing the overall survival (OS) and recurrence free survival (RFS) and make an attempt to develop a nomogram for predicting the prognosis of patients with genitourinary sarcoma (GS).

Methods: Data on adult GS from 1985 to 2010 were collected. The impact of clinical factors on OS and RFS were estimated by Kaplan-Meier (KM) analysis, and differences between groups were analyzed by the log-rank test.

Gas foaming of electrospun poly(L-lactide-co-caprolactone)/silk fibroin nanofiber scaffolds to promote cellular infiltration and tissue regeneration.

Electrospun nanofibers emulate extracellular matrix (ECM) morphology and architecture; however, small pore size and tightly-packed fibers impede their translation in tissue engineering. Here we exploited in situ gas foaming to afford three-dimensional (3D) poly(L-lactide-co-ε-caprolactone)/silk fibroin (PLCL/SF) scaffolds, which exhibited nanotopographic cues and a multilayered structure. The addition of SF improved the hydrophilicity and biocompatibility of 3D PLCL scaffolds.

Porous fish collagen for cartilage tissue engineering.

Cartilage defects repair is still a challenge in clinical practice until now. Although many breakthroughs have been achieved in cartilage repair using tissue engineering technology, there are still no scaffolds available for large-scale clinical applications. Currently, fish collagen (FC) is a natural source that is considered as an alternative to mammal-derived collagen in engineering cartilage tissue due to its excellent biocompatibility, suitable biodegradability, lack of immunogenicity, rich sources, low cost and minimal risk of transmitting zoonoses, which implies great potential for use in cartilage regeneration.

Electrospun nanofibrous membrane of fish collagen/polycaprolactone for cartilage regeneration.

Tissue engineering technology provides a promising approach for cartilage repair, and in this strategy, scaffolds play a pivotal role in directing cartilage regeneration. Fish collagen (FC) is currently considered an alternative source of mammalian collagen (MC) for tissue engineering due to its excellent biocompatibility, suitable biodegradability, inert immunogenicity, rich sources, low price and lack of risk for the transmission of zoonosis. Here, we fabricated three types of electrospun nanofibrous membranes composed of FC and polycaprolactone (PCL) with three different FC/PCL ratios (9/1, 7/3, 5/5) and investigated the feasibility of using the membranes with chondrocytes in cartilage regeneration.

Multi-omics analysis at epigenomics and transcriptomics levels reveals prognostic subtypes of lung squamous cell carcinoma.

In this study, we identified prognostic biomarkers for lung squamous cell carcinoma (LUSC) by integrating multiple sets of DNA copy number variants (CNV) and methylation variant (MET) data, and performing qPCR and immunohistochemical identification. We examined the expression of CNV and MET in 368 LUSC patients. Gene expression associated with DNA copy number or DNA methylation was identified and four LUSC gene subtypes were defined based on these correlations.

Surface modification of decellularized trachea matrix with collagen and laser micropore technique to promote cartilage regeneration.

The repair of long-segment tracheal defects remains a significant clinical challenge, to which, optimal biologically functioning tracheal alternatives may serve as a solution. Tissue-engineered trachea, regenerated from a decellularized trachea matrix using the laser micropore technique (LDTM), demonstrates the possibility of developing optimal tracheal substitutes, which retain the original tubular shape and adequate cartilage regeneration ability of trachea. However, the strict requirement with respect to the implantation cell density restricts the clinical translation of the LDTM, which has a low cell adherence rate.