Anticancer Effect of Dihydroartemisinin Dual Control of ROS-induced Apoptosis and Protective Autophagy in Prostate Cancer 22Rv1 Cells.

Background: Dihydroartemisinin (DHA), a natural agent, exhibits potent anticancer activity. However, its biological activity on prostate cancer (PCa) 22Rv1 cells has not been previously investigated.

Objectives: In this study, we demonstrate that DHA induces anticancer effects through the induction of apoptosis and autophagy.

Dihydroartemisinin induces cell apoptosis through repression of UHRF1 in prostate cancer cells.

Prostate cancer (PCa) seriously jeopardizes men's health worldwide. Dihydroartemisinin, which is an effective antimalarial agent, has shown potential anticancer effects in various human cancer cell lines, including PCa cells. However, the mechanisms underlying the anticancer activity of dihydroartemisinin are not fully understood.

Identification of miRNA signature associated with BMP2 and chemosensitivity of TMZ in glioblastoma stem-like cells.

Glioblastoma multiform (GBM) is the most lethal intracranial tumor in adults. Glioblastoma stem-like cells (GSCs) are responsible for tumorigenesis and chemotherapy resistance. BMPs are known to increase temozolomide (TMZ) response in GSCs, however, the intracellular molecular mechanism remains largely unknown.

Effect of dihydroartemisinin on UHRF1 gene expression in human prostate cancer PC-3 cells.

As the second most common cancer in men around the world, prostate cancer is increasingly gaining more attention. Dihydroartemisinin (DHA) has been proven to be a promising anticancer agent in vitro as well as in vivo in accumulating data. However, the detailed mechanisms of how DHA action in human prostate cancer PC-3 cells remain elusive.

Mechanism of dihydroartemisinin-induced apoptosis in prostate cancer PC3 cells: An iTRAQ-based proteomic analysis.

Aims: Prostate cancer (PCa) is one of the most common cancers in men in the world. Advanced PCa, especially castration-resistant PCa (CRPC), is difficult to cure. There is an urgent need to develop novel agents for CPRC.

Hypercholesterolemia increases the production of leukotriene B4 in neutrophils by enhancing the nuclear localization of 5-lipoxygenase.

Aims: Neutrophils can synthesize leukotriene B4 (LTB4) by activating the 5-lipoxygenase (5-LO)signaling pathway. LTB4 is a pro-inflammatory mediator associated with the etiology and progression of atherosclerosis. It can increase function and number of neutrophils in an autocrine manner.

[Wearable stoop-assist device in reducing incidence of low back pain].

According to human biomechanics the ideal static equilibrium model of stooped human body was built, based on which a wearable stoop-assist device (WSAD) as an intervention to reduce the load on the erector spinae was developed. Electromyography (EMG) experiments were conducted to evaluate the effectiveness of the WSAD. Results showed that the integrated EMG of the thoracic erector spinae (TES), the lumbar erector spinae (LES), the latissimus dorsi (LD) and the rectus abdominis (RA) were reduced by 43%, 48%, 32% and 14% respectively, when Sagittal trunk bent forward to 90 degrees from the vertical.

Dihydroartemisinin induces endoplasmic reticulum stress-mediated apoptosis in HepG2 human hepatoma cells.

Aims And Background: Previous studies showed that dihydroartemisinin (DHA) possessed antitumor activity in many human tumor cells through the induction of apoptosis. The aim of this study was to investigate the effects of DHA on apoptosis in the human hepatocellular carcinoma cell line HepG2 and the possible molecular mechanisms involved.

Methods: The inhibitory effect of DHA on HepG2 cells was measured by MTT assay.

[Clinical significance of histopathologic and ultrastructural pathologic examination in etiological diagnosis of infantile cholestatic diseases].

Objective: To study the features of histopathologic and ultrastructural pathologic changes of liver biopsy in patients with infantile cholestatic disease, and to investigate its diagnostic significance combining with the clinical data.

Methods: Thirty-six children diagnosed as infantile cholestatic disease and received liver biopsy in Chongqing Medical University Children's Hospital from Jun 2007 to Oct 2008 were enrolled and the pathologic and ultrastructural pathologic changes of liver were analyzed.

Results: Morphologic changes under light microscope in liver tissues included hepatocyte swelling, hepatocyte denaturation, hepatocyte necrosis, multinucleated giant cell formation, bile duct proliferation, fiber tissues proliferation and inflammatory cells infiltration in liver lobules and portal regions.

[Study on the relationship between cytosolic phospholipase A2-gamma activation and myocardial cell injury during cardiopulmonary bypass].

Objective: To investigate the relationship between cytosolic phospholipase A(2)-gamma(cPLA(2)-gamma) activation and alteration of myocardial ultrastructure during cardiopulmonary bypass (CPB) in the operation of ventricular septal defect.

Methods: Myocardial tissues from the right atria of 12 patients undergone ventricular septal defect were collected before and after CPB, cPLA(2)-gamma gene expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) and analysis was carried out using gel image analysis software. Meanwhile, alteration of myocardial ultrastructure was observed under electron microscopy.