Crystal structure of the cofactor-binding domain of the human phase II drug-metabolism enzyme UDP-glucuronosyltransferase 2B7.

Human UDP-glucuronosyltransferases (UGT) are the dominant phase II conjugative drug metabolism enzymes that also play a central role in processing a range of endobiotic compounds. UGTs catalyze the covalent addition of glucuronic acid sugar moieties to a host of therapeutics and environmental toxins, as well as to a variety of endogenous steroids and other signaling molecules. We report the 1.

[Bone mineral density in children and adolescents with idiopathic chronic musculoskeletal pain syndromes].

Purpose: There is a limited information regarding associations between bone mineral density (BMD) and idiopathic musculoskeletal pain in pediatric subjects. The aim of this cross-sectional study was to assess whether children and adolescents with pain syndrome have lower BMD than healthy subjects.

Material And Methods: Eighty-nine subjects (49 girls, 40 boys) aged 5-18 years with chronic non-rheumatic musculoskeletal pain and daily calcium consumption below 500 mg were involved in the study.

The effects of melatonin, N-acetylserotonin, and 6-hydroxymelatonin on the ultrastructure of the pinealocytes of the Syrian hamster (Mesocricetus auratus).

Objectives: The aim of this study was to examine the effects of melatonin as well as of its precursor (N-acetylserotonin) and metabolite (6-hydroxymelatonin) on the ultrastructure of the pinealocytes of the Syrian hamster.

Material And Methods: The pineal glands of 2-month-old male Syrian hamsters were examined. The animals were divided into the following groups of four animals each: group 1 - melatonin treatment; group 2 - N-acetylserotonin treatment; group 3 - 6-hydroxymelatonin treatment (all substances given subcutaneously at doses of 25 microg per animal between 16.

Youth's knowledge and attitude to epilepsy.

Purpose: The aim of this research is to assess youth's knowledge and attitude towards their epileptic peers.

Material And Methods: The present study concerned 181 students of two secondary schools in Poznań, including 107 girls and 74 boys. The tool used was a self-made questionnaire.

[Coding sequence analysis of CYP1B1 exon III in the Polish population of patients with primary congenital and juvenile glaucoma].

Several mutations of CYP1B1 gene, a member of cytochrome P450 gene family, have been associated with occurrence of primary congenital glaucoma. The aim of presented work was to investigate CYP1B1 gene exon III mutations in 72 unrelated Polish patients with primary congenital glaucoma (C), juvenile glaucoma (J) and juvenile glaucoma suspects (P). In one patient we have detected homozygous duplication of 10bp at nucleotide 8046 resulting in synthesis of truncated protein, first found among the polish population.

[Resistance to antibiotics used in Helicobacter pylori infection in children].

In literature and clinical findings, the attention has been turned to the resistance to the antibiotics used in Helicobacter pylori eradication, especially metronidazole and clarithromycin. Drug-resistance was evaluated in 50 children. Primary drug-resistance to the antibiotics used in eradications was determined in this group, and additionally, in 18 children who were subjected twice to unsuccessful eradicative therapy.

Localisation of rDNA in the Lupinus genome during the cell cycle.

Observations of a specific rDNA locus behaviour during the cell cycle were made by fluorescent in situ hybridisation (FISH) in 12 Lupinus species. Due to the pattern of chromatin de-condensation in that locus, the number of relevant sites in interphase nuclei was twice as high as the number of signals on metaphase chromosomes. The description of successive phases and an attempt of an explanation are given.

Peripheral blood lymphocyte population in children infected with Helicobacter pylori.

Purpose: Helicobacter pylori infection in children is associated with a chronic inflammatory process of gastric and duodenal mucosa, which may have a various clinical course ranging from asymptomatic and chronic inflammatory condition to gastric ulceration. The immune system may contribute especially to chronic gastric mucosa inflammation. The aim of our study was to assess the levels of peripheral blood T (CD3+, CD4+, CD8+) and B lymphocyte subpopulation (CD19+) in children with Helicobacter pylori infection and to evaluate their relation to degree of antrum mucosa inflammation.

Ultrastructure of pinealocytes in mice implanted with Colon 38 adenocarcinoma.

Objectives: Relationship between the pineal gland and neoplastic disease has been repeatedly shown in many both experimental and clinical studies. However, morphological studies of the pineal gland in animals with experimentally-induced tumors are rare. Therefore, we decided to investigate the ultrastructure of pinealocytes in mice with implanted Colon 38 adenocarcinoma.

Nitrogen-15 fractionation in the thermal decomposition of nitrous oxide of natural isotopic composition.

The 15N fractionation in the thermal decomposition of nitrous oxide (N2O) of natural isotopic composition has been investigated in quartz reaction vessel in the temperature interval 888-1073 K. The formulas relating the observed experimentally 15N fractionations with the primary 15N kinetic isotope effect, (k14/k15)p for 14N15N16O, and secondary 15N kinetic isotope effect, (k14/k15)s for 15N14N16O, have been derived. The experimentally estimated 15N kinetic isotope effects have been compared with the primary and secondary 15N kinetic isotope effects calculated with the absolute rate theory formulations applied to linear three atom molecules.

Effect of superior cervical ganglionectomy on the ultrastructure of pinealocytes in the Djungarian hamster (Phodopus sungorus): quantitative study.

Objectives: Superior cervical ganglia are of crucial importance in regulating the secretion of the pineal hormone melatonin. Changes in pineal morphology have been observed in many mammalian species after sympathectomy. Therefore, we decided to investigate the ultrastructure of Djungarian hamster pinealocytes following superior cervical ganglionectomy.

Physical mapping of 18S-25S rDNA and 5S rDNA in Lupinus via fluorescent in situ hybridization.

Double-target fluorescent in situ hybridization (FISH) was used to determine the genomic distribution of ribosomal RNA genes in five Lupinus species: L. cosentinii (2n=32), L. pilosus (2n=42), L.

Carbon isotope fractionation in the decarboxylation of phenylpropiolic acid in hydrogen donating media.

13C kinetic isotope effect (KIE) in the decarboxylation of phenylpropiolic acid (PPA) in tetralin medium (Tn) has been determined at 409-432 K and found to be of magnitude similar to the 13C KIE observed in the decarboxylation of malonic acid where the rupture of the C-C bond is the rate determining step. 13C KIE equals 1.0318/at 136 degrees C in the decarboxylation of PPA in Tn medium.

Comparative quantitative ultrastructural study of pinealocytes in eight mammalian species.

OBJECTIVES: The studies related to quantitative ultrastructural features of mammalian pinealocytes, especially in comparative aspects, are relatively rare. Quantitative ultrastructural studies in many mammalian species are lacking. Because of the existence of differences in diurnal melatonin profiles in various species it seemed interesting to compare the ultrastructure of pinealocytes in various mammalian species, both laboratory and domestic animals.

13C-CP-MAS-NMR studies of flavonoids. I. Solid-state conformation of quercetin, quercetin 5'-sulphonic acid and some simple polyphenols.

13C-CP-MAS-NMR spectra were measured in order to characterise the orientation of hydroxyl groups of quercetin, quercetin-5'-sulphonic acid and polyphenol-type compounds such as catechol, pyrogallol and gallic acid. The locked conformation of the OH group in the solid results in an increased shielding of carbon proxime to C-OH hydrogen. Carbon shieldings suggest that there is orientational disorder of three OH groups of pyrogallol and gallic acid and that two OH groups of catechol are not equivalent.

Serial transplants of 7,12-dimethylbenz[a]anthracene-induced mammary tumors in Fischer rats as model system for human breast cancer. 3. Quantitative ultrastructural studies of the pinealocytes and plasma melatonin concentrations in rats bearing an advanced passage of the tumor.

The aim of the present study was to investigate the ultrastructure of pinealocytes and plasma melatonin concentrations in Fischer rats bearing an advanced (14th) passage 7,12-dimethylbenz[a]anthracene-induced mammary tumor. Quantitative ultrastructural analysis of the cell size and relative volumes of various cell organelles as well as the number of dense-core vesicles was performed in pinealocytes of the animals killed either during the daytime (15.00 h) or at night (3.