[Isolation and characteristics of anti-adenovirus monoclonal antibodies in immunoenzyme and immunofluorescent reactions].

New monoclonal antibodies (MAbs) to adenovirus hexon, highly active in ELISA and immunofluorescent analysis, were prepared. According to competitive ELISA, new MAbs differed in their blocking activity and were directed to 2 different hexon epitopes. MAb 3H8 did not modify antigen binding of the rest MAbs labeled with peroxidase (PAb x Pox), and none of unlabeled MAbs suppressed the reaction of MAb x Pox 3H8.

[The etiological structure of the morbidity from influenza and other ARDs on the territory of Russia in the season of 1997-1998].

The antigenic properties of 51 strains of influenza virus A(H1N1), isolated in different cities of Russia during the epidemic of 1998, were studied. Most of these strains (49) proved to be similar to virus A/Bern/07/95 in the antigenic structure of hemagglutinin, but 2 strains isolated in Ulan-Ude were found to be closely related to new antigenic variants of this virus: A/Beijing/262/95 and A/Fukuoka/c7/98. The analysis of the antigenic structure of influenza-like diseases (ILD) in different cities of Russia revealed that adenoviruses causing up to 10.

[The phenotypic characteristics of healthy carriers of the antigenic structures of the influenza A virus].

Highly sensitive methods for the detection of viral antigens with the use of monoclonal or polyclonal enzyme immunoassay test systems, as well as virus-specific nucleic sequences (NS-gene) in polymerase chain reaction, made it possible to establish the fact of long-term asymptomatic virus carriership (AVC) in some of adolescent schoolchildren in a closed group. The occurrence of AVC varied at different phases of the epidemic process. The tendency to AVC was observed in individuals having blood group B (III) and HLA-A9 antigen of the major histocompatibility complex.

[Characteristics of monoclonal antibodies to RS virus in immunoenzyme and immunofluorescence techniques].

According to the competitive ELISA test data, new preparations of monoclonal antibodies (MAb) to respiratory syncytial virus (RSV) differed in their blocking activity and they were directed to 3 different virus epitopes. MAb 9C5 and MAb 131-2A (CDC, Atlanta) competed against each other strongly and they were directed to epitope F1a of RSV F-protein. MAbs 8C5 and 10D8 showed a two-way blocking and were presumably topologically linked.

[Influenza A virus antigens in children with congenital pathology of central nervous system].

The latent circulation of influenza A virus in nursing home children with congenital central nervous abnormality was studied during 1989-1993 by the present-day diagnostic methods for detection of viral antigens. The influenza A virus antigen detection rates correlated with age, genetic predisposition, prior acute respiratory diseases in mothers during their pregnancy. There is evidence for long-term (up to 17 months) detection of influenza virus antigens in nasal secretions, as well as for prolonged (up to 7 months) detection of the same genomic sequences of HA gene both in leukocytes and nasal washes.

[The results of using the molecular hybridization of nucleic acids in the complex study of nasopharyngeal smears from patients with influenza and other acute respiratory diseases].

A complex study of samples obtained from patients with influenza and other acute respiratory diseases has revealed that the laboratory methods used in this study can be rated in the following order according to their sensitivity: isolation of the virus in chick embryos, analysis of seroconversions in the hemagglutination inhibition test, immunofluorescent determination of viral antigens, determination of viral antigens by enzyme immunoassay (EIA), detection of RNA-containing viral structures by means of molecular hybridization. From the point of view of the possibility of documenting influenza A in patients, the best results are achieved by the combination of molecular hybridization and EIA techniques: 90% and more of all cases. A rational scheme for the examination of samples obtained from patients with a view to epidemiological study, including both traditional and new rapid diagnostic methods, is proposed.

[Epidemiological analysis of the influenza virus A carrier state in healthy children].

Clinical and laboratory examinations in two permanently observed children's institutions were carried out in 1968--1978. Altogether, 241 children were examined virologically 759 times, of them 181 children were found to be truly healthy. In the epidemic period the latter yielded virus in 3.

Associated seroconversions to respiratory viruses in volunteers with experimental influenza infection.

Serological examinations of 573 volunteers with mild experimental influenza infection and 86 volunteers of a control group hospitalized in a special clinic revealed a significant rise in the titre of antibodies (seroconversion) not only to influenza A or B viruses used for the experimental infection but in 23.3 to 29.8% of cases also to other respiratory viruses.