Publications by authors named "Zi-tong HUANG"

Objective: The effectiveness and safety of the short-interfering RNA drug inclisiran in lowering patients' lipoprotein cholesterol levels to lower their risk of cardiovascular disease are presently being investigated. Based on the real-world adverse event report record in the FDA Adverse Event Reporting System, this article explores the occurrence and risk of adverse events during inclisiran treatment.

Research Design And Methods: We retrieved and screened all available data from the Food and Drug Administration website for the period from 2009 to the third quarter of 2023.

View Article and Find Full Text PDF

Patchouli alcohol (PA) has been widely used for the treatment of diarrhea-predominant irritable bowel syndrome (IBS-D) in traditional Chinese medicine, and the related mechanism remains to be fully understood. Our previous study has indicated that PA significantly reduced visceral sensitivity and defecation area in IBS-D rats. In this study, we prepared an IBS-D rat model and observed the dynamic intestinal motility and colonic longitudinal muscle and myenteric plexus (LMMP) neurons, as well as their subtypes at D14, D21, and D28.

View Article and Find Full Text PDF
Article Synopsis
  • The lower urinary tract is responsible for urine storage and release, controlled by the nervous system.
  • Research on bladder neurons and glial cells has typically focused on primary cultures, but this new protocol details how to isolate and culture these cells from Sprague-Dawley rats.
  • The cultured cells were grown at 37 °C for 5-7 days, allowing for their identification and study through various staining techniques and immunocytochemistry for neurotransmitter-related proteins.
View Article and Find Full Text PDF

Hepatic fibrosis is the main pathological basis for chronic cirrhosis, and activated hepatic stellate cells (HSCs) are the primary cells involved in liver fibrosis. Our study analyzed anti-fibrosis long noncoding RNAs (lncRNAs) in activated human HSCs (hHSCs). We performed RNA sequencing (RNA-seq) and bioinformatics analysis to determine whether lncRNA expression profile changes between hHSCs activation and quiescence.

View Article and Find Full Text PDF

Aim: To elucidate the mechanism of patchouli alcohol (PA) in treatment of rat models of diarrhea-predominant irritable bowel syndrome (IBS-D).

Methods: We studied the effects of PA on colonic spontaneous motility using its cumulative log concentration (3 × 10 mol/L to 1 × 10 mol/L). We then determined the responses of the proximal and distal colon segments of rats to the following stimuli: (1) carbachol (1 × 10 mol/L to 1 × 10 mol/L); (2) neurotransmitter antagonists including N-nitro-L-arginine methyl ester hydrochloride (10 μmol/L) and (1R*, 2S*)-4-[2-Iodo-6-(methylamino)-9H-purin-9-yl]-2-(phosphonooxy)bicyclo[3.

View Article and Find Full Text PDF

Cardiac arrest-induced global cerebral ischemia is a main cause of neurological dysfunction in emergency medicine. Transplantation with bone marrow mesenchymal stem cells (MSCs) has been used in stroke models to repair the ischemic brain injury, but it is little studied in models with global cerebral ischemia. In the present study, a hypoxia precondition was used to improve the efficacy of MSC transplantation, given the low survival and migration rates and limited differentiation capacities of MSCs.

View Article and Find Full Text PDF

In this study, we investigated the effects of remote ischemic preconditioning on post resuscitation cerebral function in a rat model of cardiac arrest and resuscitation. The animals were randomized into six groups: 1) sham operation, 2) lateral ventricle injection and sham operation, 3) cardiac arrest induced by ventricular fibrillation, 4) lateral ventricle injection and cardiac arrest, 5) remote ischemic preconditioning initiated 90min before induction of ventricular fibrillation, and 6) lateral ventricle injection and remote ischemic preconditioning before cardiac arrest. Reagent of Lateral ventricle injection is neuroglobin antisense oligodeoxynucleotides which initiated 24h before sham operation, cardiac arrest or remote ischemic preconditioning.

View Article and Find Full Text PDF
Article Synopsis
  • Mitochondrial dysfunction is a key factor in brain injury following global cerebral ischemia after cardiac arrest, and carbon monoxide treatment has demonstrated protective effects against this injury.
  • A study on rats with induced cardiac arrest found that those treated with carbon monoxide had improved neurological scores and lower levels of brain injury markers compared to controls.
  • The carbon monoxide treatment was effective in reducing neuron damage and enhancing mitochondrial function by promoting the expression of factors related to mitochondrial biogenesis.
View Article and Find Full Text PDF

In the present study, mesenchymal stem cells (MSCs) were transplanted into the brain of rats following cardiopulmonary resuscitation (CPR) by three different methods: Direct stereotaxic injection into the lateral cerebral ventricle (LV), intra‑carotid administration (A), and femoral venous infusion (V). The three different methods were compared by observing the effects of MSCs on neurological function following global cerebral hypoxia‑ischemia, in order to determine the optimum method for MSC transplantation. MSCs were transplanted in groups A, V and LV following the restoration of spontaneous circulation.

View Article and Find Full Text PDF

Background: Partial pressure of end-tidal carbon dioxide (PETCO2) has been used to monitor the effectiveness of precordial compression (PC) and regarded as a prognostic value of outcomes in cardiopulmonary resuscitation (CPR). This study was to investigate changes of PETCO2 during CPR in rats with ventricular fibrillation (VF) versus asphyxial cardiac arrest.

Methods: Sixty-two male Sprague-Dawley (SD) rats were randomly divided into an asphyxial group (n=32) and a VF group (n=30).

View Article and Find Full Text PDF

Aim: To investigate the effects of mesenchymal stem cells (MSCs) transplantation on rat global cerebral ischemia and the underlying mechanisms.

Methods: Adult male SD rats underwent asphxial cardiac arrest to induce global cerebral ischemia, then received intravenous injection of 5×10(6) cultured MSCs of SD rats at 2 h after resuscitation. In another group of cardiac arrest rats, tumor necrosis factor-α-induced protein 6 (TSG-6, 6 μg) was injected into the right lateral ventricle.

View Article and Find Full Text PDF

Objective: To explore the effects and mechanisms of heme oxygenase-1 on rats with postresuscitation myocardial dysfunction.

Methods: Male Sprague-Dawley rats were asphyxiated for 9 minutes and resuscitated. They were randomly divided into 4 groups: sham-operated, cardiopulmonary resuscitation (CPR), hemin and hemin + ZnPP (zinc protoporphyrin IX).

View Article and Find Full Text PDF

Objective: To investigate the epidemiological information of patients in pre-hospital medical care in Guangzhou city, and to explore the characteristics of the patients.

Methods: The data in the year of 2008 were retrieved from the computer database of Guangzhou Emergency Medical Rescue Command Center.

Results: (1)In a total of 969 410 calls received, the time of distribution was found to be mainly between 16:00 and 18:00 [11.

View Article and Find Full Text PDF
Article Synopsis
  • * Methods: 120 rats were divided into three groups: sham-operated, resuscitation treatment (with SB-3CT), and resuscitation control (without SB-3CT), with various assessments of brain tissue conducted at multiple time points post-resuscitation.
  • * Results: The resuscitation control group showed significantly increased levels of MMP9, inflammatory markers,
View Article and Find Full Text PDF

Background: As the regulators of cytokines, suppressors of cytokine signaling (SOCS) play an important role in the inflammation reaction. Some studies found that SOCS-1 and SOCS-3 were involved in the pathogenesis of some inflammatory diseases such as rheumatoid arthritis, inflammatory bowel disease. But the expressions of SOCS in coronary heart disease have not yet been reported.

View Article and Find Full Text PDF

SARS-CoV infection of human results in antigen-specific cellular and humoral immune responses. However, it is critical to determine whether SARS-CoV-specific memory T cells can persist for long periods of time. In this study, we analyzed the cellular immune response from 21 SARS-recovered individuals who had been diagnosed with SARS in 2003 by using ELISA, CBA, ELISpot and multiparameter flow cytometry assays.

View Article and Find Full Text PDF

Objective: To explore the risk stratification and prognostic evaluation of pulmonary thromboembolism (PTE).

Methods: The clinical data of 46 patients suffering from PTE diagnosed by ventilation perfusion scan or spiral CT pulmonary angiography admitted to our hospital from January 2002 to December 2006 were analyzed retrospectively.

Results: The total mortality was 33% (15/46 cases).

View Article and Find Full Text PDF

Objective: To investigate the effect of ulinastatin on apoptosis in ileal mucosa of rats with hemorrhagic shock.

Methods: A prospective, controlled animal study was performed. The rat model of hemorrhagic shock was replicated according to method described by Chaudry.

View Article and Find Full Text PDF

The role of cell-mediated immunity in human SARS-CoV infection is still not well understood. In this study, we found that memory T-cell responses against the spike (S) protein were persistent for more than 1 year after SARS-CoV infection by detecting the production of IFN-gamma using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4(+) and CD8(+) T cells were involved in cellular responses against SARS-CoV infection.

View Article and Find Full Text PDF

Objective: To investigate the changes of the goblet cells in the intestine during the restitution process of the gut barrier after hemorrhagic shock.

Methods: Forty-nine Sprague-Dawley rats with body weight of 250-300 g were divided into control group (n=7) and experimental group (n=42). Rats in the experimental group was further divided into 6 groups (n=7 each) according to different time point at 1, 3, 6, 12, 24, and 36 hours after hemorrhagic shock resuscitation.

View Article and Find Full Text PDF

Aim: To investigate the functional, morphological changes of the gut barrier during the restitution process after hemorrhagic shock, and the regional differences of the large intestine and small intestine in response to ischemia/reperfusion injury.

Methods: Forty-seven Sprague-Dawley rats with body weight of 250-300 g were divided into two groups: control group (sham shock n = 5) and experimental group (n = 42). Experimental group was further divided into six groups (n = 7 each) according to different time points after the hemorrhagic shock, including 0(th) h group, 1st h group, 3rd h group, 6th h group, 12th h group and 24th h group.

View Article and Find Full Text PDF

Objective: To study on morphological changes in mucosa of the small and large intestine mucosa after resuscitation of hemorrhagic shock.

Methods: The morphological changes in intestinal mucosa were observed under light and electron microscope, including the histology of intestinal mucosa, determination of height of villi and evaluation of mucosa damage index in the different phases after traumatic-hemorrhagic shock.

Results: Mucosa epithelial injury was obvious in small intestine were even at 0 hour, becoming more serious in 1 hour up to 3 hours.

View Article and Find Full Text PDF

Objective: To describe a hospital outbreak of severe acute respiratory syndrome (SARS) and summarize the clinical features and therapeutic approaches.

Methods: Clinical data in this cohort were collected prospectively as they were identified.

Results: The outbreak started with a SARS patient from the community on 30 January 2003, followed by a total of 96 people [76 women and 20 men; mean age (29.

View Article and Find Full Text PDF