[Expression of miR-9 in B lymphocytes and B cell lymphomas cell lines and its significance].

Objective: To investigate the expression of miR-9 in B lymphocytes, B cell lymphoma and classical Hodgkin's lymphoma (cHL) cell lines and its significance.

Methods: CD19(+) B lymphocytes were sorted from normal lymph node by magnetic beads. Total cellular micro-RNA was extracted from cHL cell line L428, B cell lymphoma cell lines Ly1 and Ly10 (diffuse large B cell lymphoma), Raji cells (Burkitt's lymphoma) and CD19(+) B lymphocytes, respectively.

[Preparation of RNA probe for cd99l2 gene of zebrafish labeled with digoxingenin-UTP].

Objective: To study the expression pattern of cd99l2 gene during zebrafish development, the RNA probes for whole-mount in situ hybridization were prepared in this study.

Methods: The cd99l2 fragment obtained by RT-PCR was cloned into pGM-T Easy, then the plasmids were linearized with the restriction enzymes SacII or SalI. Using Sp6 or T(7) RNA polymerase, the digoxingenin-labeled antisense and sense probes were synthesized and confirmed by whole-mount in situ hybridization.

[SR-XRF analysis of characteristics of heavy element concentration in qingdao algae and application to monitoring oceanic pollution].

Quantitative and qualitative analysis of the heavy element concentration characteristics of 14 specimens of 9 kinds of Qingdao algae by synchrotron radiation X-ray fluorescence (SR-XRF) are presented. Results show that various algae are quite different in element concentration. Some algae reveal super concentration ability, such as Sargassum pallidum for As and Sr, Symphyocladia latiuscula for Br and Laminaria japonica for I.