Publications by authors named "Zi-jin Zhao"
Objective: Recombinase-aided polymerase chain reaction (RAP) is a sensitive, single-tube, two-stage nucleic acid amplification method. This study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by (SA), (PA), and (AB) in the bloodstream based on recombinant human mannan-binding lectin protein (M1 protein)-conjugated magnetic bead (M1 bead) enrichment of pathogens combined with RAP.
Methods: Recombinant plasmids were used to evaluate the assay sensitivity.
Objective: This study aimed to establish a highly sensitive and rapid single-tube, two-stage, multiplex recombinase-aided qPCR (mRAP) assay to specifically detect the khe, bla, and bla genes in Klebsiella pneumoniae.
Methods: mRAP was carried out in a qPCR instrument within 1 h. The analytical sensitivities of mRAP for khe, bla, and bla genes were tested using recombinant plasmids and dilutions of reference strains.
Article Synopsis
- Researchers developed a type of organic photodetector called broadband photomultiplication organic photodetectors (PMOPDs) using a double-layer active layer made from different blends of IEICO-4F and PBDB-T in various ratios.
- The PMOPDs are capable of detecting light from 310 nm to 930 nm, primarily influenced by the photon absorption characteristics of the 1:1 blend ratio of IEICO-4F and PBDB-T.
- The device achieves impressive performance metrics, including an external quantum efficiency over 100% due to a mechanism involving trapped electrons enhancing hole injection, along with a specific detectivity of 1.05 x 10^12 Jones and a responsivity of
Background: Metabotropic glutamate receptors (mGluRs) are G-protein-coupled receptors that mediate neuronal excitability and synaptic plasticity in the central nervous system, and emerging evidence suggests a role of mGluRs in the biology of cancer. Previous studies showed that mGluR1 was a potential therapeutic target for the treatment of breast cancer and melanoma, but its role in human glioma has not been determined.
Methods: In the present study, we investigated the effects of mGluR1 inhibition in human glioma U87 cells using specific targeted small interfering RNA (siRNA) or selective antagonists Riluzole and BAY36-7620.
Glutamate-mediated excitotoxicity is involved in many acute and chronic brain diseases. Dynamin related protein 1 (Drp-1), one of the GTPase family of proteins that regulate mitochondrial fission and fusion balance, is associated with apoptotic cell death in cancer and neurodegenerative diseases. Here we investigated the effect of downregulating Drp-1 on glutamate excitotoxicity-induced neuronal injury in HT22 cells.
View Article and Find Full Text PDFArticle Synopsis
- - The study aimed to effectively create and validate a three-dimensional finite element model of the unilateral cleft lip and palate (UCLP) craniomaxillary complex, facilitating future analyses using this method.
- - A CT scan of a 9-year-old boy with a left complete lip and palate cleft was used to generate a detailed 3D anatomical model, integrating 12 circum-maxillary sutures and achieving a highly accurate simulation with over 206,000 elements.
- - The resulting model demonstrated improved accuracy over previous finite element analysis models and was validated by comparing the displacement and stress distribution of craniofacial structures to existing literature.