Comparative chloroplast genome analysis of medicinally important (Melanthiaceae) in China: Insights into genomic characterization and phylogenetic relationships.

Members of are perennial herbs widely used in traditional Chinese medicine to induce vomiting, resolve blood stasis and relieve pain. However, the intrageneric classification and phylogenetic relationships within have long been controversial due to the complexity of morphological variations and lack of high-resolution molecular markers. In this study, we reevaluated the infrageneric relationships with the genus using complete chloroplast genome sequence data.

[Therapeutic mechanism of Psammosilene tunicoides extract on rheumatoid arthritis based on NLRP3 inflammasome].

In this study, we investigated the mechanism of crude extract of Psammosilene tunicoides(CEPT) in the treatment of rheumatoid arthritis(RA) based on the Nod-like receptor protein 3(NLRP3) inflammasome. The collagen-induced arthritis(CIA) mouse model was established. On day 32 after the primary immunization, according to the arthritis score, the mice were randomly divided into model group, positive control(methotrexate) group, low-and high-dose CEPT groups, and normal group, with 10 mice in each group.

[Selection of optimal qRT-PCR reference genes for Aconitum vilmorinianum].

Screening suitable reference genes is the premise of quantitative Real-time PCR(qRT-PCR)for gene expression analysis. To provide stable reference genes for expression analysis of genes in Aconitum vilmorinianum, this study selected 19 candidate re-ference genes(ACT1, ACT2, ACT3, aTUB1, aTUB2, bTUB, 18S rRNA, UBQ, eIF2, eIF3, eIF4, eIF5, CYP, GAPDH1, GAPDH2, PP2A1, PP2A2, ACP, and EF1α) based on the transcriptome data of A. vilmorinianum.

The complete chloroplast genome sequence of and phylogenetic analysis.

Turcz. is a medicinal plant belonging to Melanthiaceae occurring in Northeast China. However, there are still limited genomic resources available for genus .

Complete chloroplast genome sequences of (Scrophulariaceae): an Endangered species endemic to the Hengduan Mountains region.

is a perennial plant in the Scrophulariaceae family with a high value of medicinal in Tibetan medicine. In this study, we assembled and characterized the complete chloroplast genome of as a resource for future studies on this species. The chloroplast genome was 152,789 bp in size, with a large single-copy (LSC) region of 83,642 bp, a small single-copy (SSC) region of 17,795 bp, separated by two inverted repeat (IR) regions of 25,676 bp each.

The complete chloroplast genome and phylogenetic analysis of Loes. F. (Liliaceae).

Loes. F. is a medicinal plant belonging to the genus (Liliaceae).

Complete chloroplast genome sequence of (Gentianaceae): a medicinal species endemic to China.

is an important medicinal plant endemic to South-east Yunnan, China, which has been widely used to treat icteric hepatitis. The complete chloroplast genome sequence of is presented in this study, the total size is 153,015 bp in length with a typical quadripartite structure including a pair of inverted repeat (IRs, 25,786 bp) regions separated by a large single copy (LSC, 83,048 bp) region and a small single copy (SSC, 18,395 bp) region. The overall GC content of it is 38.

The complete chloroplast genome sequence of and (Asteraceae).

The complete chloroplast genomes of (Vant.) Hand-Mazz and (Lindl.) Benth were reported in this study.

The complete plastid genome sequence of (Plantaginaceae): an endangered species endemic to The Himalayas regions.

(Pennell) Hong, an endangered perennial species, is endemic to the Eastern Himalayas and Hengduan Mountains. In this study, we have sequenced the complete chloroplast genome of , which is 152,643 bp in length, including two inverted repeat (IR, 25,829 bp) regions, one large single copy region (LSC) and one small single copy region (SSC) of 83,191 bp and 17,794 bp, respectively. The cp genome has 131 annotated genes, including 86 protein-coding genes, 37 tRNA genes, and eight rRNA genes.

Characterization of the complete chloroplast genome of (Zingiberaceae).

T. L. Wu (Zingiberaceae) is a herbaceous perennial grown in Hainan Province, China, which is an important medicinal plant used for the improvement of gastrointestinal motility.

Development, characterization, and cross-amplification of microsatellite markers for (Caryophyllaceae).

Premise Of The Study: (Caryophyllaceae) is a narrowly distributed and endemic plant species in southwestern China. The overexploitation of natural has led to the destruction of many populations. Population and genetic studies will provide crucial data for the protection and management of .

[Construction of core collection of Psammosilene tunicoides based on polymorphism of β-AS gene].

Psammosilene tunicoides is one of the main ingredients of the "Yunnan Baiyao". P. tunicoides is an endangered species included in the secondary protection list in China Plant Red Data Book as well as the endemic species in Southwest China.

[Cloning of β-actin Gene in Psammosilene tunicoides and Its Role as a Reference Gene].

Objective: To establish a real-time quantitative PCR method to detect Psammosilene tunicoides β-actin, and to provide a reference gene for the detection of Psammosilene tunicoides genes by q PCR.

Methods: Specific primers were designed based on the conserved region of the β-actin gene( Gen Bank) and were used to amplify β-actin by PCR. β-actin was also used as a reference gene in the q PCR analysis of glycosyltransferase gene( UGT) expression in the roots,stems,and leaves of Psammosilene tunicoides.

Differentiation in fructification percentage between two morphs of Amomum tsaoko (Zingiberaceae).

Amomum tsaoko is a flexistylous ginger. Flexistyly is a unique floral mechanism promoting outcrossing, which is known only in some species of Zingiberaceae till date. This is a pioneer report on flexistyly in A.

[Study on content of volatile oil in Amomum tsao-ko seeds and its influencing factors].

Objective: To lay the foundation for quality evaluation of medicinal materials of Amomum tsao-ko and its selection of fine varieties, the variation of percentage of volatile oil in the seeds among populations and individuals and its influencing factors were studied.

Methods: Extracted volatile oil from the seeds according to the Chinese Pharmacopoeia, studied quantitative characters of fruit, and analyzed the data according to SPSS 13.0.

[Study on comparison of quantitative multistate character of infructescence of Amomum tsao-ko from five populations].

Objective: To study the difference of the quantitative multistate character of infructescence of Amomum tsao-ko from five populations, and the correlations between the quantitative multistate character and altitude, longitude and latitude.

Methods: By observing and analyzing the quantitative multistate character of infructescence of Amomum tsao-ko from five populations.

Results: There is highly significant difference among the number of flowerlets each inflorescence from five populations.

[Anatomy and adaptation to environment study of endangered alpine medical plant Neopicrorhiza scrophulariiflora].

Objective: To study the anatomical structure of endangered alpine medical plant Neopicrorhiza scrophulariiflora and the high altitude adaptability.

Methods: The leaf epidermis character as well as section structure of leaf, aerial stem and rhizome were observed by light microscopical technique.

Results: The leaf surface of Neopicrorhiza scrophulariiflora was covered with two kinds of glandular hair, and the stommata was anomocytic type.

[Study on quantitative multistate character of fruit and seed of Amomum tsaoko].

Objective: To explore the regulation of the growth of fruit of Amomum tsaoko and the factors influencing the weight of fruit.

Methods: To compare and analyze the quantitative multistate character of fruit and seed of Amomum tsaoko.

Results: The quantitative multistate character of fruit and seed of Amomum tsaoko in populations and between populations were abundant.

[Cloning and characterization of cDNA encoding Psammosilene tunicoides squalene synthase].

The total triterpene saponins of Psammosilene tunicoides have significant pharmacologic activity. Psammosilene tunicoides squalene synthase (PSS) is a gateway enzyme to regulate the biosynthesis of total triterpene saponins extracted from the root of Psammosilene tunicoides which is an endangered species. In this paper, cDNA encoding of PSS was cloned by the degenerate primer PCR and rapid-amplification of cDNA ends (RACE).

[Ribosomal DNA ITS sequence of analysis of Psammosilene tunicoides from different populations].

Objective: To analyze the ribosomal ITS sequence variation of Psammosilene tuncolides W. C. Wu et C.

[Growth comparison among Psammosilene tunicoides populations in tissue culture].

The comparison between the growth of eight populations from Psammosilene tunicoides at Yunnan Province was made by the tissue culture. The initial results showed out two populations from Yunshanping (Lijiang) and Xiaomoyu (Kunming) was dominant than orthers. It would be regard as one of fine germplasm resources for the culture of Psammosilence tunicoides.