regulates oxidative stress and mitophagy to maintain the balance of the follicular microenvironment in PCOS.

Objectives: To observe the CISD2 expression among PCOS patients and to explore its profound impact on the follicular microenvironment. Moreover, we want to elucidate the intricate mechanistic contribution of to the onset and progression of PCOS.

Methods: Oxidase NOX2, mitophagy-related proteins, and CISD2 were detected by WB.

Comparison of inducible versus constitutive expression of plectasin on yields and antimicrobial activities in Pichia pastoris.

Background: Plectasin might serve as a substitute for traditional antibiotics, but its yields and antimicrobial activities warrant further investigation.

Objective: To identify the influence of inducible versus constitutive expression of plectasin on yields and antimicrobial activities.

Methods: Through SOE-PCR, a recombinant plectasin gene was generated and inserted into inducible (pPICZαA) and constitutive (pGAPZαA) vectors in order to create Pichia pastoris GS115 strains.

Polymorphisms of glutathione S-transferase genes and survival of resected hepatocellular carcinoma patients.

Aim: To investigate the effects of single nucleotide polymorphisms (SNPs) in glutathione S-transferase (GST) genes on survival of hepatocellular carcinoma (HCC) patients.

Methods: Twelve tagging SNPs in GST genes (including GSTA1, GSTA4, GSTM2, GSTM3, GSTO1, GSTO2 and GSTP1) were genotyped using Sequenom MassARRAY iPLEX genotyping method in a cohort of 214 Chinese patients with resected HCC. The Cox proportional hazards model and log-rank test were performed to determine the SNPs related to outcome.