Cloning and characterization of an oxiranedicarboxylate hydrolase from Labrys sp. WH-1.

Objective: This study aimed to clone and characterize the oxiranedicarboxylate hydrolase (ORCH) from Labrys sp. WH-1.

Methods: Purification by column chromatography, characterization of enzymatic properties, gene cloning by protein terminal sequencing and polymerase chain reaction (PCR), and sequence analysis by secondary structure prediction and multiple sequence alignment were performed.

The aroma volatile repertoire in strawberry fruit: a review.

Aroma significantly contributes to flavor, which directly affects the commercial quality of strawberries. The strawberry aroma is complex as many kinds of volatile compounds are found in strawberries. In this review, we describe the current knowledge of the constituents and of the biosynthesis of strawberry volatile compounds, and the effect of postharvest treatments on aroma profiles.

[Changes in cell wall component metabolism and ultrastructure of postharvest persimmon fruit during softening].

The changes in cellular wall hydrolases, cellular wall components and cellular wall ultrastructure of postharvest persimmon (Diospyros kaki L. cv. Bianhua) fruit during softening were studied.