Establishment and Modification of Ninety-seven Pneumococcal Serotyping Assays Based on Quantitative Real-time Polymerase Chain Reaction.

Objective: To establish and modify quantitative real-time polymerase chain reaction (qPCR)-based serotyping assays to distinguish 97 pneumococcal serotypes.

Methods: A database of capsular polysaccharide ( ) loci sequences was generated, covering 97 pneumococcal serotypes. Bioinformatics analyses were performed to identify the loci structure and target genes related to different pneumococcal serotypes with specific SNPs.

Comparison of 0.025% FK-506, 0.05% Cyclosporin A, and 0.3% Sodium Hyaluronate Eye Drops for the Treatment of Botulinum Toxin B-Induced Mouse Dry Eye.

Purpose: To compare the effects of FK-506, cyclosporin A (CsA), and sodium hyaluronate (HA) eye drops for the treatment of botulinum toxin B (BTX-B)-induced mouse dry eye.

Methods: CBA/J mice were randomized into 5 groups. The groups received treatment with eye drops containing 0.

Self-assembly and anion sensing of metal-organic [ML] cages from fluorescent triphenylamine tri-pyrazoles with dipalladium(ii,ii) corners.

Three hexametal-organic cages [(N^N)PdL] with syn, syn, syn conformation were synthesized via the coordination of tris(4-(1H-pyrazol-3-yl)phenyl)amine ligands and [(N^N)Pd(NO)](NO) dimetallic corners (N^N = 2,2'-bipyridine for 1; 4,4'-dimethylbipyridine for 2; 1,10-phenanthroline for 3). These hexametal-organic cages exhibit anion sensing toward HSO in aqueous solution via fluorescent titration and NMR spectroscopy studies.

Programmable self-assembly of water-soluble organo-heterometallic cages [MM'L] using 3-(3,5-dimethyl-1H-pyrazol-4-yl)pentane-2,4-dione (HL).

A bifunctional ligand HL featuring primary (pyrazole) and secondary (acetylacetone) coordination sites was preferentially reacted with dimetallic [M(NO)](NO) linkers at the pyrazolyl end of HL, giving rise to dimetallic corners. Subsequently, the corners serve as the secondary site with M' to form water-soluble organo-heterometallic [MM'L] cages in a stepwise mode.