Publications by authors named "Zi-Chao Yu"
Article Synopsis
- The study focuses on developing a cold-adapted expression system using Pseudoalteromonas to overcome limitations faced by the commonly used Escherichia coli system, particularly for thermolabile proteins.
- Researchers created a shuttle vector and established a conjugational transfer system between E. coli and the psychrophilic strain Pseudoalteromonas sp. SM20429 to facilitate protein expression.
- The successful expression of the cold-adapted protease, pseudoalterin, at low temperatures demonstrates that this new expression system is effective for proteins that are difficult to express in E. coli, offering a promising alternative for biotechnological applications.
Although bacteriobenthos play a major role in the degradation of particulate organic matter in marine sediment, knowledge of the sediment-adapted lifestyles of bacteriobenthos is still scarce. Here, the particle-associated, swimming and swarming lifestyles of the benthonic bacterium Pseudoalteromonas sp. SM9913 (SM9913) were illustrated.
View Article and Find Full Text PDFSea ice is one of the most frigid environments for marine microbes. In contrast to other ocean ecosystems, microbes in permanent sea ice are space confined and subject to many extreme conditions, which change on a seasonal basis. How these microbial communities are regulated to survive the extreme sea ice environment is largely unknown.
View Article and Find Full Text PDFBackground: Pseudoalteromonas species are a group of marine gammaproteobacteria frequently found in deep-sea sediments, which may play important roles in deep-sea sediment ecosystem. Although genome sequence analysis of Pseudoalteromonas has revealed some specific features associated with adaptation to the extreme deep-sea environment, it is still difficult to study how Pseudoalteromonas adapt to the deep-sea environment due to the lack of a genetic manipulation system. The aim of this study is to develop a genetic system in the deep-sea sedimentary bacterium Pseudoalteromonas sp.
View Article and Find Full Text PDFA Gram-stain-negative, aerobic, oxidase- and catalase-positive, flagellated, rod-shaped bacterial strain, designated SM1222(T), was isolated from the deep-sea sediment of the South China Sea. The strain grew at 4-35 °C and with 0.5-8 % NaCl (w/v).
View Article and Find Full Text PDFArticle Synopsis
- Pseudoalteromonas is a marine genus with psychrophilic strains that thrive in cold environments; however, research is hindered by a lack of effective genetic systems.
- This study developed a new heterologous expression system using a cryptic plasmid pSM429 from Arctic Pseudoalteromonas, enabling genetic manipulations.
- Key findings included the characterization of replication initiation proteins essential for the plasmid's functionality and successful expression of antibiotic resistance and cold-adapted enzyme genes in a Pseudoalteromonas strain.