Tissue-specific metabolite profiling of benzylisoquinoline alkaloids in the root of Macleaya cordata by combining laser microdissection with ultra-high-performance liquid chromatography/tandem mass spectrometry.

Rationale: Tissue-specific metabolite profiling helps to find trace alkaloids masked during organ analysis, which contributes to understanding the alkaloid biosynthetic pathways in vivo and evaluating the quality of medical plants by morphology. As Macleaya cordata contains diverse types of benzylisoquinoline alkaloids (BIAs), the alkaloid metabolite profiling was carried out on various tissues of the root.

Methods: Laser microdissection with fluorescence detection was used to recognize and dissect different tissues from the root of M.

[Chemical Constituents from the Roots of Macleaya cordata].

Objective: To investigate the chemical constituents from the roots of Macleaya cordata.

Methods: The compounds were isolated and purified by silica gel,recrystallization and semi-preparative HPLC, their structures were eclucidated by physicochemistry properties, MS and NMR.

Results: Seven compounds were isolated and identified as 6-cyanodihydrochelerythrine (1),6-cyanodihydrochelilutine (2),dihydrochelirubine (3),6-methoxynorchelerythrine (4),dihydrosanguinarine (5),6-actonyldihydrosanguinarine (6) and stigmasterol (7).

[Study on Alkaloids of Fruit from Macleaya cordata].

Objective: To study the alkaloids from the fruit of Macleaya cordata.

Methods: The alkaloids were isolated and purified by silica gel column chromatography and preparative high performance liquid chromatography,and the structures were elucidated based on the spectral data.

Results: Eight alkaloids were obtained from the fraction of ethanol extract and identified as 6-cyanodihydrosanguinarine( 1),6-acetonyldihydrosanguinarine( 2),6-acetonyldihydrochelerythrine( 3),chelerythrine( 4),dihydrochelerythrine( 5),13,14-dehydrogen-N-methyl-coptisine( 6),N-methyl-canadine( 7) and berberine( 8).