Transcriptome-wide mapping of internal mRNA N-methylguanosine in sporulated and unsporulated oocysts of Eimeria tenella reveals stage-specific signatures.

Background: Growing evidence indicates that N-methylguanosine (mG) modification plays critical roles in epigenetic regulation. However, no data regarding mG modification are currently available in Eimeria tenella, a highly virulent species causing coccidiosis in chickens.

Methods: In the present study, we explore the distribution of internal messenger RNA (mRNA) mG modification in sporulated and unsporulated oocysts of E.

mRNA 5-methylcytosine in Eimeria tenella oocysts: An abundant post-transcriptional modification associated with broad-ranging biological processes.

Eimeria tenella is the major causative agent of chicken coccidiosis. 5-Methylcytosine (mC) is a type of RNA chemical modifications reported to regulate diverse biological processes. However, the distribution and biological functions of mC in E.

Rhodium-catalyzed three-component C(sp)/C(sp)-H activation enabled by a two-fold directing group strategy.

Rh-catalyzed three-component C(sp)/C(sp)-H activation has been achieved through a two-directing group strategy. This protocol provides a convenient and efficient pathway for the construction of diverse 8-alkyl quinoline derivatives in one-pot. Furthermore, mechanistic studies revealed that the first C-H amidation was significantly faster than the sequential C-H alkylation.

A CARM1 Inhibitor Potently Suppresses Breast Cancer Both In Vitro and In Vivo.

CARM1, belonging to the protein arginine methyltransferase (PRMT) family, is intricately associated with the progression of cancer and is viewed as a promising target for both cancer diagnosis and therapy. However, the number of specific and potent CARM1 inhibitors is limited. We herein discovered a CARM1 inhibitor, iCARM1, that showed better specificity and activity toward CARM1 compared to the known CARM1 inhibitors, EZM2302 and TP-064.

Research Note: Preliminary functional analysis of EGF-like domains of Eimeria tenella microneme protein 7 identified in sporozoites and merozoites.

Eimeria tenella, an obligate intracellular apicomplexan parasite, is the major causative agent of chicken coccidiosis. Some epidermal growth factor (EGF)-like domain-containing proteins of other members of apicomplexan parasites have been reported to contribute to parasite survival. To date, however, EGF-like domain-containing proteins of E.

Metabolomic and transcriptomic profiling reveals the effect of dietary protein and lipid levels on growth performance in loach ().

The subject of this study was to explore the optimum requirements of loach () regarding dietary proteins and lipids and discuss the underlying mechanism. We designed nine diets to determine the effects of different levels of dietary crude protein (CP: 30%, 35%, and 40%) and ether extract (EE: 6%, 10%, and 14%) on the growth performance and metabolism of . In total, 2160 healthy (5.

Seroprevalence of Toxoplasma gondii infection in sheep and cattle in Shanxi Province, North China.

Toxoplasmosis is a worldwide zoonotic disease caused by infection with the intracellular protozoan parasite Toxoplasma gondii, posing significant economic losses to the livestock industry. As a major livestock province, little is known of the prevalence of T. gondii infection in sheep and cattle in Shanxi Province, North China.

Relationship between pyroptosis-mediated inflammation and the pathogenesis of prostate disease.

The largest solid organ of the male genitalia, the prostate gland, is comprised of a variety of cells such as prostate epithelial cells, smooth muscle cells, fibroblasts, and endothelial cells. Prostate diseases, especially prostate cancer and prostatitis, are often accompanied by acute/chronic inflammatory responses or even cell death. Pyroptosis, a cell death distinct from necrosis and apoptosis, which mediate inflammation may be closely associated with the development of prostate disease.

CuTi-oxo clusters functionalized by - generated 2,2'-biphenolate ligands from the oxidative coupling of phenols.

Herein, we report a twisted cubic Cu(I)Ti(IV)-oxo cluster stabilized by - formed 2,2'-biphenolate ligands from the oxidative coupling of phenols. The 2,2'-biphenolate-functionalized Cu(I)Ti(IV)O cluster shows short Cu⋯C contacts and exhibits smaller HOMO-LUMO gaps than those of reported Ti(IV)O.

Transcriptomic analysis of LMH cells in response to the overexpression of a protein of encoded by the locus ETH_00028350.

A protein of (encoded by the locus ETH_00028350) homologous to dense granule protein 9, designated as EtHGRA9 hereafter, was reported to be expressed in all life cycle stages of . However, no data are currently available regarding its functional properties. In the present study, a recombinant vector harboring a 741 bp gene segment encoding the mature form of EtHGRA9 was constructed and transfected into leghorn male hepatoma (LMH) cells.

Progress in traditional Chinese medicine and natural extracts for the treatment of lupus nephritis.

Lupus nephritis (LN) is an autoimmune disease with multiple system involvement and is also one of the most serious forms of organ damage in systemic lupus erythematosus (SLE), which is mainly caused by the formation and deposition of immune complexes in glomeruli. More than 50% of SLE patients have clinical manifestations of renal damage. At present, the treatment of lupus nephritis is mainly based on glucocorticoids and immunosuppressants.

Observational Insights into Isoprene Secondary Organic Aerosol Formation through the Epoxide Pathway at Three Urban Sites from Northern to Southern China.

Isoprene is the most abundant precursor of global secondary organic aerosol (SOA). The epoxide pathway plays a critical role in isoprene SOA (iSOA) formation, in which isoprene epoxydiols (IEPOX) and/or hydroxymethyl-methyl-α-lactone (HMML) can react with nucleophilic sulfate and water producing isoprene-derived organosulfates (iOSs) and oxygen-containing tracers (iOTs), respectively. This process is complicated and highly influenced by anthropogenic emissions, especially in the polluted urban atmospheres.

[Effects of landscape complexity and local management on bee pollinator diversity in apple orchards in Changping District, Beijing, China.].

Pollinators provide important ecosystem services for crop production and food security. With the development of agricultural economy and the increasing intensity of land-use, a large number of natural or semi-natural habitats have been converted to croplands. Landscape homogenization and intensive management lead to the decline of wild bee diversity and threaten the sustainable agricultural production.

Fast writer adaptation with style extractor network for handwritten text recognition.

Writing style is an abstract attribute in handwritten text. It plays an important role in recognition systems and is not easy to define explicitly. Considering the effect of writing style, a writer adaptation method is proposed to transform a writer-independent recognizer toward a particular writer.

Immune Cell Landscape in Gastric Cancer.

Background: The tumor-infiltrating immune cells are closely associated with the prognosis of gastric cancer (GC). This article is aimed at determining the composition change of immune cells and immune regulatory factors in GC and normal tissues, depicting their prognosis value in GC, and revealing the relationship between them and GC clinical parameters.

Methods: We used CIBERSORT to calculate the proportion of 22 immune cells in the GC or normal tissues; a -test was applied to assess the expression difference of immune cells and immune regulatory factors in normal and GC tissues.

Experimental and Theoretical Studies on Effects of Structural Modification of Tin Nanoclusters for Third-Order Nonlinear Optical Properties.

Tin oxide based materials have attracted much attention as new sources for nonlinear optical (NLO) devices, while the electronic mechanism behind the structure and nonlinearity is still unclear. In this work, by precisely controlling different functionalization ligands, here a series of binuclear [(BuSn)(TEOA)L] (L = monocarboxylic acid ligand) complexes have been synthesized and characterized; we also adopted a new method to make the metal clusters and PMMA blend together for NLO testing. Importantly, the electronic structure, static third-order NLO properties, sum over states (SOS) have been studied by both experimental and density function theory (DFT) analysis.

Effects of Er:YAG laser pre-treatment on dentin structure and bonding strength of primary teeth: an in vitro study.

Background: To investigate the effects of Er:YAG laser pre-treatment on the dentin structure and shear bond strength of primary teeth.

Methods: Dentin specimens were prepared using freshly extracted intact primary molars and divided randomly into four groups based on the surface treatment applied. The control and etchant groups received no treatment and conventional acid etching treatment, respectively, while the energy and frequency groups received laser surface treatment with variable energy (50-300 mJ) and frequency (5-30 Hz) parameters.

[Variation characteristics of soil seed bank during vegetation restoration in red soil erosion area].

As a natural disturbance agent, soil erosion could affect secondary distribution and species composition of soil seed bank. The composition, storage and distribution pattern of the soil seed banks in five different vegetation recovery areas, including bare ground (1), pine forest land (2-4) and secondary forest (5) in the typical red soil erosion area, were studied to explore the effects of soil erosion on soil seed bank during vegetation restoration. The results showed that a total of 21 species were recorded in the soil seed bank.

A hypermethylation strategy utilized by enhancer-bound CARM1 to promote estrogen receptor α-dependent transcriptional activation and breast carcinogenesis.

While protein arginine methyltransferases (PRMTs) and PRMT-catalyzed protein methylation have been well-known to be involved in a myriad of biological processes, their functions and the underlying molecular mechanisms in cancers, particularly in estrogen receptor alpha (ERα)-positive breast cancers, remain incompletely understood. Here we focused on investigating PRMT4 (also called coactivator associated arginine methyltransferase 1, CARM1) in ERα-positive breast cancers due to its high expression and the associated poor prognosis. : ChIP-seq and RNA-seq were employed to identify the chromatin-binding landscape and transcriptional targets of CARM1, respectively, in the presence of estrogen in ERα-positive MCF7 breast cancer cells.

Endobronchial ultrasound-guided transbronchial needle aspiration can improve the diagnostic accuracy of positron emission tomography/computed tomography in hilar and/or mediastinal lymphadenopathy.

Context: Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) and positron emission tomography/computed tomography (PET/CT) are the two most extensively used methods for the diagnosis and staging of lung cancer.

Aims: The present study was designed to compare the diagnostic performance of EBUS-TBNA with that of PET/CT in patients with hilar and/or mediastinal lymphadenopathy.

Settings And Design: We compared the accuracy of EBUS-TBNA with that of PET/CT in the diagnosis of hilar and/or mediastinal lymphadenopathy and evaluated the diagnostic utility of EBUS-TBNA in patients with PET/CT false-positive and false-negative findings.