TDMPP activation of estrogen receptor 2a regulates smc2 and p53 signaling to interfere with liver development in zebrafish (Danio rerio).

Tris (2,6-dimethylphenyl) phosphate (TDMPP), a novel organic phosphorus flame retardant (OPFR), has been found to have estrogenic activity. Estrogens are critical in regulating various biological responses during liver development. However, the effects of TDMPP on zebrafish liver development remain largely unexplored.

Prospects and challenges of CAR-T cell therapy combined with ICIs.

Immune checkpoint molecules are a group of molecules expressed on the surface of immune cells that primarily regulate their immune homeostasis. Chimeric antigen receptor (CAR) T cell therapy is an immunotherapeutic technology that realizes tumor-targeted killing by constructing synthetic T cells expressing specific antigens through biotechnology. Currently, CAR-T cell therapy has achieved good efficacy in non-solid tumors, but its treatment of solid tumors has not yielded the desired results.

Research Progress and Direction of Novel Organelle-Migrasomes.

Migrasomes are organelles that are similar in structure to pomegranates, up to 3 μm in diameter, and contain small vesicles with a diameter of 50-100 nm. These membranous organelles grow at the intersections or tips of retracting fibers at the back of migrating cells. The process by which cells release migrasomes and their contents outside the cell is called migracytosis.

Application value of whole exome sequencing in screening and identifying novel mutations of hypopharyngeal cancer.

The research on targeted therapy of hypopharyngeal cancer is very scarce. The discovery of new targeted driver genes will promote the progress of hypopharyngeal cancer therapy to a great extent. In our research, whole-exome sequencing in 10 patients with hypopharyngeal cancer was performed to identify single nucleotide variations (SNVs) and insertions and deletions (INDELs).

Regulating the Expression of HIF-1α or lncRNA: Potential Directions for Cancer Therapy.

Previous studies have shown that tumors under a hypoxic environment can induce an important hypoxia-responsive element, hypoxia-induced factor-1α (HIF-1α), which can increase tumor migration, invasion, and metastatic ability by promoting epithelial-to-mesenchymal transition (EMT) in tumor cells. Currently, with the deeper knowledge of long noncoding RNAs (lncRNAs), more and more functions of lncRNAs have been discovered. HIF-1α can regulate hypoxia-responsive lncRNAs under hypoxic conditions, and changes in the expression level of lncRNAs can regulate the production of EMT transcription factors and signaling pathway transduction, thus promoting EMT progress.

The potential, analysis and prospect of ctDNA sequencing in hepatocellular carcinoma.

Background: The genome map of hepatocellular carcinoma (HCC) is complex. In order to explore whether circulating tumor cell DNA (ctDNA) can be used as the basis for sequencing and use ctDNA to find tumor related biomarkers, we analyzed the mutant genes of ctDNA in patients with liver cancer by sequencing.

Methods: We used next-generation targeted sequencing technology to identify mutations in patients with liver cancer.

Nasal Nanovaccines for SARS-CoV-2 to Address COVID-19.

COVID-19 is still prevalent around the globe. Although some SARS-CoV-2 vaccines have been distributed to the population, the shortcomings of vaccines and the continuous emergence of SARS-CoV-2 mutant virus strains are a cause for concern. Thus, it is vital to continue to improve vaccines and vaccine delivery methods.

Triple Frustrated Lewis Pair-Type Reactivity on a Single Rare-Earth Metal Center.

Rare-earth metal cations have been used rarely as Lewis-acidic components in the chemistry of frustrated Lewis pairs (FLPs). Herein, we report the first cerium/phosphorus system (2) employing a heptadentate N P ligand, which exhibits triple FLP-type reactivity towards a series of organic substrates, including isocyanates, isothiocyanates, diazomethane, and azides on a single rare-earth Lewis acidic Ce center. This result shows that the Ce center and three P atoms in 2 could simultaneously activate three equivalents of small molecules under mild conditions.

An Unprecedented Ga/P Frustrated Lewis Pair: Synthesis, Characterization, and Reactivity.

Frustrated Lewis pairs (FLPs) represent a new paradigm of main-group chemistry. The Lewis acidic centers in FLP chemistry are typically B and Al atoms in the studies reported over the past decade, and most of them are tri-coordinated with strong electron-withdrawing groups. Herein, a Ga/P system is reported which contains an unprecedented four-coordinated Lewis acidic Ga center.

Cd-induced apoptosis was mediated by the release of Ca2+ from intracellular Ca storage.

Previous studies found that cadmium (Cd) could induce apoptosis via interfering with the intracellular calcium (Ca) ions homeostasis. But the detailed mechanisms remain poorly understood. In the present study two cell lines (normal human liver cell HL-7702, and tumor cell Raji cell) were exposed to Cd along or co-incubated with ethylene glycol-bis (2-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA) and 1,2-bis (2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetoxymethyl ester (BAPTA-AM), respectively.

Assessment of the genotoxicity in toad Bufo raddei exposed to petrochemical contaminants in Lanzhou Region, China.

Single cell gel electrophoresis or comet assay, micronucleus (MN) test and global DNA methylation detection were used to assess the genotoxicity in toad Bufo raddei exposed to the petrochemical (mainly oil and phenol) polluted area in Lanzhou Region (LZR) comparing with a relatively unpolluted area in Liujiaxia Region (LJXR). The results from the present study indicated that DNA damage and MN frequency in toad from LZR were significantly higher than those from LJXR at the same sampling month, whereas the degree of global DNA methylation was lower, which implies that the petrochemical contaminants at environmental level in LZR were genotoxic to B. raddei.