[Hyperosmotic stress and perfusion culture strategies increase the yield of recombinant adenoviral vector produced by HEK 293 cells].

With various diseases ravaging internationally, the demands for recombinant adenoviral vector (Adv) vaccines have increased dramatically. To meet the demand for Adv vaccine, development of a new cell culture process is an effective strategy. Applying hyperosmotic stress in cells before virus infection could increase the yield of Adv in batch culture mode.

New azaphilones and tremulane sesquiterpene from endophytic Nigrospora oryzae cocultured with Irpex lacteus.

Five new metabolites belonging to two backbones of pulvilloric acid-type azaphilone and tremulane sesquiterpene were obtained and their structures were determined by spectral analysis. Based on the biogenesis analysis, tremulane sesquiterpenes were obtained from Irpex lacteus by the stimulation of mixed-culture. The antifungal selectivities of metabolites produced by fungus against their co-culture fungus and common pathogens, exhibited competitive interaction of this mix-culture.

New Azaphilones from Co-Cultured with .

In this study, the co-culture of and , the endophytes in the seeds of , were examined for metabolite diversity. Five new azaphilones were isolated, and their structures were determined by spectral analysis. In terms of azaphilones, compound had an unprecedented skeleton, with a bicyclic oxygen bridge.

Benzopyran derivatives from endophytic JC-15 in and their bioactivities.

Five new benzopyran derivatives (-) and a new natural product () were isolated from endophytic in and determined as (R)-2,3-dihydro-2,5-dihydroxy-2-methylchromen-4-one (), (2R, 4S)-2,3-dihydro-2-methyl-benzopyran-4,5-diol (), (R)-3-methoxyl-1-(2,6-dihydroxy phenyl)-butan-1-one (), 7-O-α-d-ribosyl-5-hydroxy-2-methyl-4H-chromen-4-one (), 7-O-α-d-ribosyl-2,3-dihydro-5-hydroxy-2-methyl-chromen-4-one (), daldinium A (). These compounds were evaluated for their antimicrobial activity, anti-acetylcholinesterase, nitric oxide inhibition, anticoagulant, photodynamic antimicrobial activities and glucose uptake of adipocytes. Some compounds showed photoactive antimicrobial activities and glucose uptake stimulating activities.

New bioactive compounds from aquatic endophyte Chaetomium globosum.

Two new oxidation products-related aureonitol and cytochalasan were isolated from Chaetomium globosum fermented in Chinese yam (Dioscorea opposita) and determined as 10,11-dihydroxyl- aureonitol (1) and yamchaetoglobosin A (2). Compound 2 indicated significant inhibitory effect on nitric oxide production in LPS-activated macrophages, anti-acetylcholinesterase activity with the inhibition ratios of 92.5, 38.