Publications by authors named "Zhuosi Li"

This study aimed to evaluate the virulence characteristics of ST11 Salmonella enterica from various sources and explore its pathogenic mechanisms and the molecular basis of antimicrobial resistance. In total, 20 Salmonella isolates collected between 2017 and 2022 from environmental, animal, clinical, and food sources were analyzed. Comprehensive investigations were conducted using whole-genome sequencing, bioinformatic analysis, broth microdilution methods, a two-dimensional (2D) cell model (Caco-2 cells), and a Galleria mellonella infection model.

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Salmonella Typhimurium monophasic variant (STm) is an emerging Salmonella serotype that causes food poisoning through the contamination of various foods, including animal products, vegetables, and fruits. In particular, the increasing prevalence of multidrug-resistant STm highlights the urgent need to develop effective strategies to control this pathogen. In this work, a novel and broad-spectrum Salmonella phage named vB_Sal_TmvP009 was isolated and characterized.

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Food microbial contamination results in serious food safety issues and numerous food loss and waste, presenting one of the most significant challenges facing the global food system. Photodynamic inactivation (PDI) technology, which combines light and photosensitizers (PS) to provide antimicrobial effects, is an ideal nonthermal antimicrobial technique for the food industry. This review provides a comprehensive overview of PDI technology, beginning with the fundamental photoactivation principles of PS and the pathways of photoinduced reactive oxygen species (ROS) generation.

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is one of the most important foodborne pathogens that can cause invasive listeriosis. In this study, the virulence levels of 26 strains of isolated from food and clinical samples in Shanghai, China, between 2020 and 2022 were analyzed. There were significant differences among isolates in terms of their mortality rate in , cytotoxicity to JEG-3 cells, hemolytic activity, and expression of important virulence genes.

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Listeria monocytogenes is a critical foodborne pathogen known to develop adaptation traits in mildly acidic food processing environments. This study analyzed the genomic data of 49 strains derived from clinical and food sources, utilizing genome-wide association studies (GWAS) to explore the correlation between the genotypic and phenotypic traits of L. monocytogenes, thereby identifying the genetic determinants of its acid adaptation capability.

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Salmonella as a foodborne pathogen, has a very important impact on human health. This study characterized the serotype, plasmid replicon, MLST typing, resistance phenotype and resistance gene of 282 strains of Salmonella isolated from the environment, animals, foods, and patients in China. These strains are classified into four sources: poultry farms (39/282), animals (30/282), foods (164/282), and hospitals (49/282) their resistance to ciprofloxacin, levofloxacin and ceftazidime is gradually increasing.

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The viable but nonculturable (VBNC) state occurs when bacteria lose their ability to grow and multiply on conventional media when stressed by adverse environmental factors, but they remain active and can revive under certain conditions, posing a food safety risk. In this study, the VBNC state of Listeria monocytogenes was induced with different temperatures combined with low nutrient conditions; the VBNC state of L. monocytogenes was confirmed in conjunction with the housekeeping gene abcZ using a molecular biology assay (PMA-qPCR) to calculate the viable bacterial count; The resuscitation conditions for the VBNC state of L.

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Listeria monocytogenes is a common foodborne pathogen that frequently causes global outbreaks. In this study, the growth characteristics, biofilm formation ability, motility ability and whole genome of 26 L. monocytogenes strains isolated from food and clinical samples in Shanghai (China) from 2020 to 2022 were analyzed.

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Listeria monocytogenes is an important foodborne pathogen that causes listeriosis, a severe and fatal condition. Biofilms are communities of microorganisms nested within a self-secreted extracellular polymeric substance, and they protect L. monocytogenes from environmental stresses.

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Article Synopsis
  • Foodborne pathogens pose significant safety issues, leading to increased interest in effective bacteriostatic agents.
  • Sesamol (SE), found in sesame oil, has demonstrated antibacterial properties and potential health benefits, specifically against various foodborne pathogens.
  • The study revealed that SE effectively inhibited the growth of five pathogenic bacteria by altering their cell membranes and morphology, suggesting its potential use as a food safety agent.
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Article Synopsis
  • A study in Shanghai, China, found that 32.87% of ready-to-eat fruits and vegetables tested positive for a specific foodborne pathogen, indicating significant prevalence across different types.
  • Among the samples, many isolates showed resistance to common antibiotics such as trimethoprim-sulphamethoxazole, oxacillin, and ampicillin, with a total of 15 antibiotic resistance genes identified.
  • Researchers discovered seven multi-locus sequence types, including two new types, and confirmed the existence of methicillin-resistant strains, suggesting serious implications for food safety and a potentially dangerous level of antibiotic resistance.
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is recognized as a significant foodborne pathogen, capable of causing listeriosis in humans, which is a global public health concern. This pathogen is particularly dangerous for pregnant women, as it can lead to invasive listeriosis in fetuses and neonates, posing a significant threat to both maternal and fetal health. Therefore, establishing suitable and models for .

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Aims: Disinfectants such as benzalkonium chloride (BC), extensively used in animal farms and food-processing industries, contribute to the development of adaptive and cross-resistance in foodborne pathogens, posing a serious threat to food safety and human health. The purpose of this study is to explore whether continuous exposure of Salmonella enterica serovar 1,4,[5],12:i:- (S. 1,4,[5],12:i:-) to sublethal concentrations of BC could result in acquired resistance to this agent and other environmental stresses (e.

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Listeria monocytogenes is a Gram-positive bacterium frequently involved in food-borne disease outbreaks and is widely distributed in the food-processing environment. This work aims to depict the impact of nutrition deficiency on the survival strategy of L. monocytogenes both in planktonic and biofilm states.

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Contaminations of pathogenic and spoilage microbes on foods are threatening food safety and quality, highlighting the importance of developing antimicrobial agents. According to different working mechanisms, the antimicrobial activities of yeast-based agents were summarized from two aspects: antagonism and encapsulation. Antagonistic yeasts are usually applied as biocontrol agents for the preservation of fruits and vegetables via inactivating spoilage microbes, usually phytopathogens.

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Listeria monocytogenes is a foodborne pathogen causing serious public health problems. Nisin is a natural antimicrobial agent produced by Lactococcus lactis and widely used in the food industry. However, the anti-L.

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Listeria monocytogenes is a ubiquitous organism that can be found in food-related environments, and sanitizers commonly prevent and control it. The aim of this study is to perform a meta-analysis of L. monocytogenes response to sanitizer treatments.

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Listeria monocytogenes biofilm is a consistent source of cross-contamination, both in housing storage and food processing environments. This study monitored the dynamic process of L. monocytogenes ST9 and ST87 biofilms, as well as their cross-contamination behaviors at various stages of formation.

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Listeria monocytogenes is an important food-borne pathogen, which could be detected in food, environmental and clinical samples. It contaminates food in any of the stages during production, processing, and storage, resulting in potential food safety issues. Traditional physical and chemical methods are effective in inhibiting the growth of L.

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Listeria monocytogenes is a major foodborne pathogen that can adhere to or form a biofilm on food contact surfaces, depending on the environmental conditions. The purpose of this work is to determine the adhesion and biofilm formation boundaries for L. monocytogenes ST9 under the combination environments of temperature (5, 15, and 25 °C), NaCl concentration (0%, 3%, 6%, and 9% (w/v)) and pH (5.

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This study aims to investigate the mono- and dual-species biofilm formation of Listeria monocytogenes and Pseudomonas aeruginosa incubated in different culture mediums, inoculum ratios, and incubation time. The planktonic cell population and motility were examined to understand the correlation with biofilm formation. The results showed that chicken juice significantly inhibited the biofilm formation of L.

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(LM) is a food-borne pathogen that can cause listeriosis. Pregnant women are main target population of listeriosis due to pregnancy-associated immune deficiency and unique intracellular infection ability of LM to non-phagocytic cells. LM can cross the placental barrier and cause significant harm to the fetus, including premature birth, miscarriage and even stillbirth.

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The importance of single-cell variability is increasingly prominent with the developments in foodborne pathogens modeling. Traditional predictive microbiology model cannot accurately describe the growth behavior of small numbers of cells due to individual cell heterogeneity. The objective of the present study was to develop predictive models for single cell lag times of Salmonella Enteritidis after heat and chlorine treatment.

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is a foodborne pathogen responsible for many food outbreaks worldwide. This study aimed to investigate the single and combined effect of fructooligosaccharides (FOS) and subsp. CICC 6257 () on the growth, adhesion, invasion, and virulence of gene expressions of 19112 serotype 4b ().

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Foodborne disease caused by is an important public health concern worldwide. Animal-based food, especially poultry meat, is the main source of human salmonellosis. The objective of this study was to evaluate the prevalence and epidemiology of contamination in raw poultry meat commercialized in China.

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