[Adsorption, separation, and purification of cyclosporine A using reversed-phase liquid chromatography].

High performance liquid chromatography (HPLC) is widely used in the separation and analysis of cyclosporine A (CsA). Analyzing the chromatographic behavior of CsA is key to the purification of CsA by preparative HPLC. In this study, the retention behavior of CsA on the C18 column using mobile phases of methanol-water and acetonitrile-water was compared.

Isolation of achiral aliphatic acid derivatives from Piper kadsura using preparative two-dimensional chiral supercritical fluid chromatography.

The separation of structural analogues in natural products has always been one of the challenges in separation science, where supercritical fluid chromatography (SFC) with chiral stationary phases (CSPs) is an unconventional but potential solution. In this study, a preparative two-dimensional chiral SFC (2D cSFC) method that was established with two kinds of CSPs was applied in the isolation of the aliphatic acid derivatives in Piper kadsura (P. kadsura).

Separation and characterization of phenylamides from Piper kadsura using preparative supercritical fluid chromatography and ultra-high-performance supercritical fluid chromatography-tandem mass spectrometry.

A preparative supercritical fluid chromatography method for the separation of Piper kadsura obtained five phenylamide compounds, which had the same structural skeleton, but changed in the number and position of methoxyl substituents. To improve the separation selectivity of these structural analogues, silica, phenyl, and chiral stationary phases were screened. Only through the combination of Chiral C and phenyl columns could the separation of the five phenylamides be solved.

A ternary eluent strategy to tune the peak shape of steviol glycosides in reversed-phase liquid chromatography.

In this paper, an effective strategy of using acetonitrile-methanol-water as mobile phase was developed to achieve acceptable peak shape of steviol glycosides in reversed-phase liquid chromatography (RPLC). The change of elution profiles of rebaudioside A (RA) was systematically investigated. Two classical distributions, namely, tailing and fronting peaks resulting from injections of RA solution in range of 0.

Bioactivity-guided separation of antifungal compounds by preparative high-performance liquid chromatography.

Bioactivity-guided chromatographic methods are of great significance for the isolation of the active compounds in complex samples. In this study, four anti-fungal compounds were located by activity screening and successfully isolated from a microbial fermentation sample by preparative high-performance liquid chromatography. Separation performance of columns including C18, positively charged C18, negatively charged C18 and C8 were firstly investigated.

Isolation of three polyoxins by reversed-phase liquid chromatography with pure aqueous mobile phase.

Developing methods for the isolation of highly polar compounds from complex samples is of great significance. In this study, three polyoxins were successfully isolated from a complex sample (PN1-1 ) by preparative high-performance liquid chromatography. Separation was carried out on five polar reversed-phase stationary phases, using pure aqueous as mobile phase, where the C18HC column can provide the best performance for PN1-1 .

Exploration and optimization of conditions for quantitative analysis of lignans in Schisandra chinensis by an online supercritical fluid extraction with supercritical fluid chromatography system.

An online supercritical fluid extraction with supercritical fluid chromatography system could provide sequential extraction and quantitative analysis of lignans in Schisandra chinensis. Supercritical fluid extraction conditions were optimized at 15 MPa, 50°C, and 4 min with supercritical CO adding 1% methanol; the elution volume and flow rate were set at 6 mL and 2 mL/min to blow extract out of the tank completely. The split-flow rate was confirmed at 2.

Design, synthesis and evaluation of a series of alkylsiloxane-bonded stationary phases for expanded supercritical fluid chromatography separations.

Supercritical fluid chromatography (SFC) today represents an alternative technique in analytical chemistry due to its obvious benefits in kinetic performance and its complementarity to liquid chromatography. In this paper, a series of alkylsiloxane-bonded stationary phases were synthesized and evaluated to expand their SFC applications. Five kinds of non-endcapped C8 stationary phases (C8-1 to C8-5) with increasing bonding density were synthesized, and the carbon content was 3.

Rapid purification of diastereoisomers from Piper kadsura using supercritical fluid chromatography with chiral stationary phases.

Supercritical fluid chromatography (SFC) with chiral stationary phases (CSPs) is an advanced solution for the separation of achiral compounds in Piper kadsura. Analogues and stereoisomers are abundant in natural products, but there are obstacles in separation using conventional method. In this paper, four lignan diastereoisomers, (-)-Galbelgin, (-)-Ganschisandrin, Galgravin and (-)-Veraguensin, from Piper kadsura were separated and purified by chiral SFC.