Publications by authors named "Zhuoliang He"

The RING finger (RNF) family, a group of E3 ubiquitin ligases, plays multiple essential roles in the regulation of innate immunity and resistance to viral infection in mammals. However, it is still unclear whether RNF proteins affect the production of IFN-I and the replication of avian influenza virus (AIV) in ducks. In this article, we found that duck RNF216 (duRNF216) inhibited the duRIG-I signaling pathway.

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During avian influenza virus (AIV) infection, host defensive proteins promote antiviral innate immunity or antagonize viral components to limit viral replication. UFM1-specific ligase 1 (UFL1) is involved in regulating innate immunity and DNA virus replication in mammals, but the molecular mechanism by which chicken (ch)UFL1 regulates AIV replication is unclear. In this study, we first identified chUFL1 as a negative regulator of AIV replication by enhancing innate immunity and disrupting the assembly of the viral polymerase complex.

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Magnetic resonance imaging (MRI) demonstrates clear advantages over other imaging modalities in neurosurgery with its ability to delineate critical neurovascular structures and cancerous tissue in high-resolution 3D anatomical roadmaps. However, its application has been limited to interventions performed based on static pre/post-operative imaging, where errors accrue from stereotactic frame setup, image registration, and brain shift. To leverage the powerful intra-operative functions of MRI, e.

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Article Synopsis
  • Waterfowl, especially ducks, are key carriers of avian influenza viruses (AIVs), which can be transmitted to chickens and humans; since 2013, H5N6 subtype AIVs have posed a significant threat to poultry in China.
  • This study focused on analyzing the genetic evolution, transmission, and pathogenicity of these H5N6 viruses found in southern China, revealing that they possess characteristics indicative of high pathogenicity and belong to various genotypes.
  • The research demonstrated that while the H5N6 viruses caused severe illness and death in chickens, ducks experienced mild symptoms without mortality, indicating differences in how these species respond to the infection and highlighting important implications for outbreak prevention.
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  • Since 2017, H7N9 highly pathogenic avian influenza viruses have caused significant chicken infections and deaths in China, with over 200,000 cases and 120,000 fatalities.
  • A study analyzed several H7N9 strains from Guangdong, revealing they were highly pathogenic and genetically diverse, indicating genetic reassortment.
  • Experiments with the Q24 and Q26 viruses showed they killed inoculated chickens within days and triggered strong immune responses, highlighting their effective replication and transmission capabilities.
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Mitochondrial antiviral signaling protein (MAVS) is a key adaptor in cellular innate immunity. Ubiquitination plays an important role in regulating MAVS-mediated innate immune responses; however, the molecular mechanisms underlying ubiquitination of MAVS have not been fully elucidated. In this study, we first identified the mitochondria-resident E3 ligase duck membrane-associated RING-CH 8 (duMARCH8) in ducks as a negative regulator of duck MAVS (duMAVS).

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The innate immune response is a host defense mechanism that induces type I interferon and proinflammatory cytokines. Tripartite motif (TRIM) family proteins have recently emerged as pivotal regulators of type I interferon production in mammals. Here, we first identified duck TRIM29, which encodes 571 amino acids and shows high sequence homology with other bird TRIM29 proteins.

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Magnetic resonance (MR) imaging (MRI) provides compelling features for the guidance of interventional procedures, including high-contrast soft tissue imaging, detailed visualization of physiological changes, and thermometry. Laser-based tumor ablation stands to benefit greatly from MRI guidance because 3D resection margins alongside thermal distributions can be evaluated in real time to protect critical structures while ensuring adequate resection margins. However, few studies have investigated the use of projection-based lasers like those for transoral laser microsurgery, potentially because dexterous laser steering is required at the ablation site, raising substantial challenges in the confined MRI bore and its strong magnetic field.

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The H5N6 highly pathogenic avian influenza virus (HPAIV) has been circulating in China since 2013. In this report, we describe our recent chicken experimental studies investigating the pathogenicity and transmission of four H5N6 HPAIV field strains of different origins (GS39, CK44, DK47 and CK74) and the host immune responses. Four-week-old specific-pathogen-free chickens were inoculated intranasally with one of the four H5N6 HPAIV strains (one strain per group).

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The protein inhibitor of the activated STAT2 (PIAS2) has been implicated in many cellular processes and can also regulate viral replication in mammals. However, the role of PIAS2 in the highly pathogenic avian influenza virus (HPAIV) H5N1 replication in ducks is still unclear. Through liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay, we identified that duck PIAS2 (duPIAS2) was one protein that interacted with the nucleoprotein (NP) from the H5N1 HPAIV strain of DK212.

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In mammals, tripartite motif 32 (TRIM32) is essential for regulating host innate immune responses to viral infections. However, the antiviral effect of TRIM32 in birds has not been reported. Here, we cloned the full-length duck TRIM32 (duTRIM32) cDNA from total spleen RNA of Peking duck.

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The protein inhibitor of activated STAT (PIAS) proteins are important signal transduction modulator family and regulate the innate immune signaling pathway induced by certain transcription factors, including NF-κB, IRF3, and JAK/STAT. The PIAS protein mechanism that regulates innate immune response in mammals has been well described in the literature; however, whether the PIAS gene exists in ducks as well as the role of PIAS in duck IFN-β expression is still unclear. Here, we cloned duck PIAS (duPIAS), finding PIAS2 could repress IFN-β production.

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H5Nx viruses have continuously emerged in the world, causing poultry industry losses and posing a potential public health risk. Here, we studied the phylogeny, pathogenicity, transmission, and immune response of four H5N6 avian influenza viruses in chickens and mice, which were isolated from waterfowl between 2013 and 2014. Their HA genes belong to Clade 2.

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Article Synopsis
  • There have been five outbreaks of the H7N9 influenza virus in humans in China since its emergence in 2013, leading to over 1,000 infections.
  • The H7N9 virus mutated from a low pathogenic strain to a highly pathogenic strain by altering four amino acids in the HA protein, causing 15 outbreaks in chickens since late 2016.
  • In experiments, a highly pathogenic H7N9 strain (Q29) showed greater mortality and viral replication in chickens compared to a low pathogenic strain (A46), indicating increased pathogenicity and a potential ongoing threat to both poultry and human health.
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Article Synopsis
  • H5 highly pathogenic avian influenza viruses (HPAIVs) of clade 2.3.4.4 have been circulating in Southern China since 2014, causing significant losses in the poultry industry.
  • A study analyzed the genetic characteristics of seven H5N6 strains from 2016, revealing diverse genotypes and evidence of genetic recombination among some strains.
  • The viruses showed high pathogenicity, with infected chickens dying within a few days and replicating in multiple tissues, indicating the need for enhanced monitoring and control measures for avian influenza in the region.
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Highly pathogenic avian influenza H5N6 viruses have been circulating in poultry in Asia since 2013 and producing serious diseases in chickens. Here, we analyzed the genetic properties of 10 H5N6 subtypes AIVs from geese in 2015-2016 in Guangdong province. Phylogenic analysis showed that all HA genes of the 10 viruses belonged to clade 2.

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