Hsa_circ_0005548 knockdown repairs OGD/R-induced damage in human brain microvascular endothelial cells via miR-362-3p/ETS1 axis.

Background: Ischemic stroke (IS) is a highly prevalent type of stroke with very high rates of disability and death. As the regulatory role of circular RNAs (circRNAs) in various diseases has been revealed, we constructed a stroke cell model to analyze the action mechanism of hsa_circ_0005548 in IS.

Methods: The abundance of hsa_circ_0005548, microRNA-362-3p (miR-362-3p) and E26 transformation specific-1 (ETS-1) were measured by real-time quantitative polymerase chain reaction (RT-qPCR) or western blot.

Long non-coding RNAs (lncRNAs) and microRNAs regulatory pathways in the tumorigenesis and pathogenesis of glioma.

A number of long non-coding RNAs (lncRNAs) have been identified to play an important role in the initiation and progression of glioma, including the stemness, survival, apoptosis, invasion, and drug resistance. However, the complex regulatory mechanisms of lncRNAs have not been well understood in glioma. Emerging evidence has confirmed that lncRNAs may act as a competing endogenous RNA (ceRNA) or a molecular sponge in modulating microRNAs (miRNAs), and the miRNAs negatively regulate target mRNA expression through complementarity binding to the 3'-untranslated region (3'UTR) of their target mRNAs.