Publications by authors named "Zhuohong He"
Objective: The pathogenesis of GDM and T2DM are closely related to various metals in vivo, and changes in the concentration of these metal exposures can lead to neuropathy through the DNA damage pathway caused by the accumulation of ROS.
Method: Urine samples were analyzed for heavy metals and trace elements by ICP-MS, neurotransmitter metabolites by HPLC, 8-OH-dG by HPLC-MS and metabolomics by UPLC-MS.
Result: Cd and Hg were risk factors for T2DM.
Article Synopsis
- The study focuses on developing an in-line Raman spectroscopy method to monitor key parameters like glucose, lactate, and viable cell density (VCD) during the commercial production of biosimilar adalimumab using Chinese hamster ovary (CHO) cells in a 1500 L bioreactor.
- The researchers optimized Raman calibration models to achieve low prediction errors for glucose, lactate, and VCD, making the method effective for real-time monitoring of the cell culture process.
- Additionally, the study explored how the number of batches used for model calibration affects model performance, providing insights for similar bioprocess monitoring in the future.