A MoS nanosheet-based CRISPR/Cas12a biosensor for efficient miRNA quantification for acute myocardial infarction.

Acute myocardial infarction (AMI) represents the leading cause of cardiovascular death worldwide, and it is thus pivotal to develop effective approaches for the timely detection of AMI markers, especially possessing the characteristics of antibody-free, signal amplification, and manipulation convenience. We herein construct a MoS nanosheet-powered CRISPR/Cas12a sensing strategy for sensitive determination of miR-499, a superior AMI biomarker to protein markers. The presence of miR-499 at a trace level is able to induce a significantly enhanced fluorescence signal in a DNA-based molecular engineering platform, which consists of CRISPR/Cas12a enzymatic reactions and MoS nanosheet-controllable signal reporting components.

Tuning the Covalent Coupling Degree between the Cathode and Electrolyte for Optimized Interfacial Resistance in Solid-State Lithium Batteries.

The development of promising solid-state lithium batteries has been a challenging task mainly due to the poor interfacial contact and high interfacial resistance at the electrode/solid-state electrolyte (SSE) interface. Herein, we propose a strategy for introducing a class of covalent interactions with varying covalent coupling degrees at the cathode/SSE interface. This method significantly reduces interfacial impedances by strengthening the interactions between the cathode and SSE.

Bio-inspired aptamers decorated gold nanoparticles enable visualized detection of malathion.

Biosensors always respond to the targets of interest in a specific manner, employing biological or bio-mimic recognition elements such as antibodies and aptamers. Inspired by target recognition in nature, an aptamer-mediated, gold nanoparticle-based sensing approach is developed in this work for effective determination of malathion. The sensing system consists of negatively charged aptamer probes, and polycationic proteins, protamine, as well as exceptional colorimetric nanoprobes, barely gold nanoparticles (AuNPs).

DNA-templated copper nanocluster: A robust and universal fluorescence switch for bleomycin assay.

Bleomycin (BLM) is widely utilized for cancer treatment due to the outstanding antitumor activity, but BLM with imprecisely controlled dosage may lead to lethal consequences. It is thus a profound task to accurately monitor the BLM levels in clinical settings. Herein, we propose a straightforward, convenient, and sensitive sensing method for BLM assay.

Rational design of an allosteric G-quadruplex aptamer probe for ultra-sensitive detection of melamine in milk.

Efficient and accurate detection of melamine in dairy products remains a crucial yet challenging task. Herein, an allosterically modulated G-quadruplex-integrated aptamer is rationally designed with thymine-rich recognition termini for melamine binding. The detection process is facile by simply introducing the analyte into the mixture consisting of G-quadruplex aptamer probes, exonuclease III, and thioflavin T (ThT).

A label-free and signal-amplifiable assay method for colorimetric detection of carcinoembryonic antigen.

In this work, an innovative colorimetric assay method for the determination of carcinoembryonic antigen is developed with aptamer probes utilized as recognition element. DNA hybridization chain reaction is used as signal amplification technique, and peroxidase-mimicking hemin/G-quadruplex-assisted catalytic oxidation of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) is deployed as signal reporting mechanism. The detection principle was firstly verified by using gel electrophoresis analysis and absorbance measurements.

Engineering of nanonetwork-structured carbon to enable high-performance potassium-ion storage.

Potassium-ion batteries (KIBs) have been developed as an emerging electrochemical energy storage device due to the low cost and abundant resource of potassium. However, they suffer insufficient cyclability and poor rate capability caused by the large K, severely limits their further applications. Herein, a nanonetwork-structured carbon (NNSC) is reported to address the issue.