Production of succinate with two CO fixation reactions from fatty acids in Cupriavidus necator H16.

Background: Biotransformation of CO into high-value-added carbon-based products is a promising process for reducing greenhouse gas emissions. To realize the green transformation of CO, we use fatty acids as carbon source to drive CO fixation to produce succinate through a portion of the 3-hydroxypropionate (3HP) cycle in Cupriavidus necator H16.

Results: This work can achieve the production of a single succinate molecule from one acetyl-CoA molecule and two CO molecules.