PLSCR1 suppresses SARS-CoV-2 infection by downregulating cell surface ACE2.

Unlabelled: Type I interferons exert their antiviral effects against SARS-CoV-2 by inducing the expression of interferon-stimulated genes (ISGs), including but not limited to LY6E, CH25H, IFITM2/3, and IFIH1. However, the antiviral effect and underlying mechanisms of action of most ISGs in SARS-CoV-2 infection are not yet fully understood. By screening 109 ISG-knockout cell lines, we identify that phospholipid scramblase 1 (PLSCR1), an interferon-inducible protein, acts as a crucial restriction factor against SARS-CoV-2 infection.

Synthesis of Degradable Mainchain Semifluorinated Polyester by CuAAC with Reaction-Enhanced Reactivity of Intermediate (RERI) Mechanism.

Quantitative insertion of functionality into polymer chains via step-growth polymerization is challenging due to the random bimolecular reaction and relatively low extent of reaction. Herein, a copper-catalyzed azide-alkyne cycloaddition (CuAAC) step-growth polymerization with reaction-enhanced reactivity of intermediates (RERI) mechanism is applied for the precise insertion of degradable functions into the mainchain semifluorinated polymer and endowed it with controlled degradability. In this CuAAC polymerization, bis-alkynyl-terminated (A) monomer can be quantitatively consumed when the slightly excess of 2,2-bis(azidomethyl)propane-1,3-diyl bis(2-methylpropanoate) (BiAz, B) monomer with RERI effect is employed.

Structural edge damage detection based on wavelet transform and immune genetic algorithm.

The wavelet transform (WT) has gained significant attention for its ability to identify damage details within strain modes. However, edge damage in structures often remains obscured and unrecognizable when WT is applied, primarily due to edge effects. Intelligent algorithms used to assess structural damage severity often face challenges such as premature convergence and a tendency to settle on local optima.

RTP4 restricts influenza A virus infection by targeting the viral NS1 protein.

The influenza A virus evades the host innate immune response to establish infection by inhibiting RIG-I activation through its nonstructural protein 1 (NS1). Here, we reported that receptor-transporting protein 4 (RTP4), an interferon-stimulated gene (ISG), targets NS1 to inhibit influenza A virus infection. Depletion of RTP4 significantly increased influenza A virus multiplication, while NS1-deficient viruses were unaffected.

Total immunoglobulin E levels in induced sputum reflect asthma control status.

Background: Most patients with severe asthma are sensitized to at least one allergen. Whether local immunoglobulin E (IgE) in induced sputum reflects asthma control status has not been investigated.

Methods: Patients with asthma were classified as well controlled, partly controlled, and uncontrolled asthma (UCA) according to Global Initiative for Asthma 2022 guidelines.

Mapping functional elements of the DNA damage response through base editor screens.

Maintaining genomic stability is vital for cellular equilibrium. In this study, we combined CRISPR-mediated base editing with pooled screening technologies to identify numerous mutations in lysine residues and protein-coding genes. The loss of these lysine residues and genes resulted in either sensitivity or resistance to DNA-damaging agents.

Overview of evidence-based research on acupuncture for stroke treatment using magnetic resonance imaging technology.

Background: Stroke is a neurological condition characterized by high rates of disability and mortality. Magnetic resonance imaging (MRI) is widely used to examine the mechanisms of acupuncture in stroke treatment.

Purpose: This review provides neuroimaging evidence for the efficacy of acupuncture in treating stroke using MRI.

Blocking TSP1 Ameliorates Diabetes Mellitus-Induced Erectile Dysfunction by Inhibiting the TGF-β/SMAD Pathway.

Purpose: To examine the role and mechanism of thrombospondin-1 (TSP1) in the development of fibrosis in diabetes mellitus-induced erectile dysfunction (DMED).

Materials And Methods: DMED was induced by intraperitoneal streptozotocin injection. All rats were categorized into three groups: control group (n=8), DMED group (n=8) and DMED+Leu-Ser-Lys-Leu (LSKL) group (n=8).

Functional profiling of serine, threonine and tyrosine sites.

Systematic perturbation of amino acids at endogenous loci provides diverse insights into protein function. Here, we performed a genome-wide screen to globally assess the cell fitness dependency of serine, threonine and tyrosine residues. Using an adenine base editor, we designed a whole-genome library comprising 817,089 single guide RNAs to perturb 584,337 S, T and Y sites.

Kinetically Controlled Preparation of Worm-like Micelles with Tunable Diameter/Length and Structural Stability.

Anisotropic nanoparticles such as worm-like micelles have aroused much attention due to their promising applications from templates to drug delivery. The fabrication of worm-like micelles with tunable structural stability and control over their diameter and length is of great importance but still challenging. Herein, we report a kinetically controlled ring-opening metathesis polymerization-induced self-assembly (ROMPISA) for the robust preparation of kinetically trapped worm-like micelles with tunable diameter/length at enlarged experimental windows by the rational manipulation of kinetic factors, including solvent property, temperature, and π-π stacking effects.

Anaerobic hydrocarbon biodegradation by alkylotrophic methanogens in deep oil reservoirs.

In subsurface biodegraded oil reservoirs, methanogenic biodegradation of crude oil is a common process. This process was previously assigned to the syntrophy of hydrocarbon-degrading bacteria and methanogenic archaea. Recent studies showed that archaea of the Candidatus Methanoliparum named as alkylotrophic methanogens couple hydrocarbon degradation and methane production in a single archaeon.

Cultivation of novel Atribacterota from oil well provides new insight into their diversity, ecology, and evolution in anoxic, carbon-rich environments.

Background: The Atribacterota are widely distributed in the subsurface biosphere. Recently, the first Atribacterota isolate was described and the number of Atribacterota genome sequences retrieved from environmental samples has increased significantly; however, their diversity, physiology, ecology, and evolution remain poorly understood.

Results: We report the isolation of the second member of Atribacterota, Thermatribacter velox gen.

[Clinical analysis and genetic diagnosis of three children with Isoleucine metabolic disorders due to variants of HSD17B10 and ACAT1 genes].

Objective: To explore the clinical, biochemical and genetic characteristics of three children with Isoleucine metabolic disorders due to variants of HSD17B10 and ACAT1 genes.

Methods: Two children with 17β hydroxysteroid dehydrogenase 10 (HSD17B10) deficiency and a child with β-ketothiolase deficiency (BKD) diagnosed at Shanghai Children's Hospital between 2014 and 2021 were selected as the study subjects. Clinical data of the children were collected.

Comparative analysis of inherited metabolic diseases in normal newborns and high-risk children: Insights from a 10-year study in Shanghai.

Background: Compare the differences between normal newborns and high-risk children with inherited metabolic diseases. The disease profile includes amino acidemias, fatty acid oxidation disorders, and organic acidemias.

Methods: Data was collected on newborns and children from high-risk populations in Shanghai from December 2010 to December 2020.

Efficacy of acupuncture as an adjunctive treatment to patients with stable COPD: a multicenter, randomized, sham-controlled trial protocol.

Background: Chronic obstructive pulmonary disease (COPD) is a common respiratory disease and the third leading cause of death worldwide. Previous evidence has shown that acupuncture may be an effective complementary alternative therapy for stable COPD. However, large-sample, rigorously designed long-term follow-up studies still need to be completed.

Unbiased interrogation of functional lysine residues in human proteome.

CRISPR screens have empowered the high-throughput dissection of gene functions; however, more explicit genetic elements, such as codons of amino acids, require thorough interrogation. Here, we establish a CRISPR strategy for unbiasedly probing functional amino acid residues at the genome scale. By coupling adenine base editors and barcoded sgRNAs, we target 215,689 out of 611,267 (35%) lysine codons, involving 85% of the total protein-coding genes.

Historical development of karst evergreen broadleaved forests in East Asia has shaped the evolution of a hemiparasitic genus (Orobanchaceae).

is a shrubby genus of about eight species distributed basically in East Asian evergreen broadleaved forests (EBLFs), with distribution centers in the karst regions of Yunnan, Guizhou, and Guangxi in southwestern China. Based on the hemiparasitic and more or less liana habits of this genus, we hypothesized that its evolution and distribution were shaped by the development of EBLFs there. To test our hypothesis, the most comprehensive phylogenies of hitherto were constructed based on plastome and nuclear loci (nrDNA, PHYA and PHYB); then divergence time and ancestral areas were inferred using the combined nuclear loci dataset.

Acupuncture as adjunctive therapy for patients with AECOPD: study protocol for a multicenter, randomized controlled trial.

Background: The acute exacerbation of chronic obstructive pulmonary disease (AECOPD) is a common respiratory disease among older adults, which imposes a significant burden on individuals and society and poses a major challenge to the global public health system due to its high morbidity and mortality. Acupuncture is effective for AECOPD, but its efficacy has been questioned due to the limited methodological quality. Thus, we aim to investigate the efficacy of acupuncture as adjunctive therapy for AECOPD and determine whether the efficacy of acupuncture differs with the type of acupoint combinations.

Structurally Diverse Cytotoxic Polyphenols from .

Thirty-five diverse polyphenols, belonging to seven structure classes, were isolated from , a medicinal and edible plant sampled from Laos. The structures of nine new compounds, gargarcilones A-I (-, -, , , and ), were established using spectroscopic, X-ray diffraction, and experimental and calculated ECD methods. Additionally, we revised the stereochemical assignment of cochinchinoxanthone and cochinchinoxanthone C.

A wavelet based data coupling method for spatial damage detection in beam-type structures.

Spatial damage identification is of great significance in mechanical, aerospace, and civil engineering. In this study, a data coupling method based on continuous wavelet transform (CWT) is proposed to identify the spatial damage location of beam-type structures. The singularity of the wavelet coefficient can be used to identify the signal singularity, and data coupling method calculates the spatial location of the damage.

Integrated interactome and transcriptome analysis reveals key host factors critical for SARS-CoV-2 infection.

The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has seriously threatened global public health and caused huge economic losses. Omics studies of SARS-CoV-2 can help understand the interaction between the virus and host, thereby providing a new perspective in guiding the intervention and treatment of the SARS-CoV-2 infection. Since large amount of SARS-CoV-2 omics data have been accumulated in public databases, this study aimed to identify key host factors involved in SARS-CoV-2 infection through systematic integration of transcriptome and interactome data.

Dual-Recommendation Disentanglement Network for View Fuzz in Action Recognition.

Multi-view action recognition aims to identify action categories from given clues. Existing studies ignore the negative influences of fuzzy views between view and action in disentangling, commonly arising the mistaken recognition results. To this end, we regard the observed image as the composition of the view and action components, and give full play to the advantages of multiple views via the adaptive cooperative representation among these two components, forming a Dual-Recommendation Disentanglement Network (DRDN) for multi-view action recognition.

The high performance parameterization for deep learning in pulse shaping.

In high energy physics, front-end data acquisition systems based on analog-to-digital converters (ADC) can provide multiple aspects (time, energy, position) of information when an incident particle is detected. To process the shaped semi-Gaussian pulses from ADCs, multi-layer neural networks (aka. deep learning recently) show excellent accuracy and promising real-time capability.