Flow Cytometry for Assessing Human Sperm Acrosomal Reaction (AR) and Correlation of AR and Acrosomal Enzyme Activity With Semen Parameters.

Background/aim: This study aimed to evaluate human sperm acrosomal reaction (AR) using flow cytometry and examine the correlation of AR and acrosomal enzyme activity (AEA) with seven semen parameters: total sperm count, concentration, vitality, motility, morphology, survival rate, and sperm DNA fragmentation index (DFI).

Patients And Methods: A retrospective analysis was conducted on semen data from 398 men in the AR group and 526 men in the AEA group. AR was assessed using flow cytometry, while AEA was measured with the solid-phase BAPNA method.