Differential centrifugation in culture and differentiation of rat neural stem cells.

(1) The study of neural stem cells (NSC) has attracted much attention in recent years because of their therapeutic potential. However, the problem in culture and differentiation of NSC was how to obtain single cell suspension that preserves the function of NSC, and remove the debris caused by mechanical dissociation. In the present study, we try to find a simple and effective way to address the problem, i.

[The analysis of differential expression and cloning of genes related to raised secondary lateral veins mutant of Lycoris aurea].

Lycoris aurea exhibits parallel venation, the main vein with many lateral veins in a longitudinal parallel arrangement. There are secondary lateral veins (SLV) between each longitudinal veins. In general, SLVs are not remarkable.

[Preparation, characterization and preliminary application of recombinant protein AP33 of trichomonas vaginalis].

Objective: To clone ap33 gene of Trichomonas vaginalis( T. v), construct prokaryotic expression system of the gene and identify its antigenicity and immunogenicity.

Methods: The total RNA was extracted from a clinical isolate Tv317 and the cDNA was synthesized by reverse transcription.

Bromocriptine treatment of invasive giant prolactinomas involving the cavernous sinus: results of a long-term follow up.

Object: The aim of this study was to observe long-term clinical outcomes in a group of patients treated with bromocriptine for invasive giant prolactinomas involving the cavernous sinus.

Methods: Data from 20 patients with invasive giant prolactinomas at the authors' institutions between July 1997 and June 2004 were retrospectively reviewed. The criteria to qualify for study participation included: (1) tumor diameter greater than 4 cm, invading the cavernous sinus to an extent corresponding to Grade III or IV in the classification scheme of Knosp and colleagues; (2) serum prolactin (PRL) level greater than 200 ng/ml; and (3) clinical signs of hyperprolactinemia and mass effect.

[Identification of markers linked to resistance locus of Marssonina leaf spot in poplars by bulked segregant analysis(BSA)].

DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis(BSA). The bulks consisted of individual with a extreme phenotype taken from a population of 91 F1 clones,which is a progeny of Populus deltoides Bartr.cv.

Identification and expression analysis of EST-based genes in the bud of Lycoris longituba.

To obtain a primary overview of gene diversity and expression pattern in Lycoris longituba, 4,992 ESTs (Expressed Sequence Tags) from L. longituba bud were sequenced and 4,687 cleaned ESTs were used for gene expression analysis. Clustered by the PHRAP program, 967 contigs and 1,343 singlets were obtained.

[Phylogeny of the genus Arundinaria based on nucleotide sequences of nrDNA ITS region].

The sequences of nrDNA regions of 17 species and Phyllostachys edulis (outgroup) sampled, which are of represent and type species for different taxa in the genus Arundinaria,were analyzed by PCR amplification and direct DNA sequencing. The phylogenetic trees generated from maximum parsimony analysis showed that the sampled bamboos were naturally monophletic, appearing that these species of the bamboos belong to the genus Arundinaria. The internal transcribed spacers (ITS) data indicated that the species were divided into two branches, one including A.

[Feasibility study of controlled hypotension induced with combination of propofol and desflurane in craniotomy].

Objective: To evaluate the feasibility of controlled hypotension induced with combination of propofol and desflurane in craniotomy.

Methods: Thirty-five ASA I approximately II patients undergoing elective craniotomy were randomly selected. Anesthetic induction was achieved with midazolam, thiopental, fentanyl, and vecuronium.

[Analysis of the inter-species relationships on lycoris Amaryllidaceae) by use of RAPD].

The fingerprints of 13 species in genus Lycoris were generated by use of RAPD method. Forty-one primers were screened from 520 random primers, and a total of 350 DNA fragments were amplified ranging from 0.3-3.

[Sequence of the ITS region of nuclear ribosomal DNA(nrDNA) in Xinjiang wild Dianthus and its phylogenetic relationship].

Xinjiang is a center of distribution and differentiation of genus Dianthus in China, and has a great deal of species resources. The sequences of ITS region (including ITS-1, 5.8S rDNA and ITS-2) of nuclear ribosomal DNA from 8 species of genus Dianthus wildly distributed in Xinjiang were determined by direct sequencing of PCR products.

Molecular linkage maps of the Populus genome.

We report molecular genetic linkage maps for an interspecific hybrid population of Populus, a model system in forest-tree biology. The hybrids were produced by crosses between P. deltoides (mother) and P.

[Microsurgical treatment of cranial base meningioma].

Objective: To improve the total removal rate of cranial base meningiomas, and to reduce the mortality and morbidity.

Methods: 103 patients with cranial base meningiomas were treated by microsurgery. Tumors of different position were resected by variant transcranial base approaches and the operative methods.

[Microsurgical treatment of foramen magnum tumors].

Objectives: To review the radiological features, and results of microsurgical treatment of foramen magnum tumors.

Methods: We retrospectively analyzed the results of microsurgical treatment of 31 patients with foramen magnum tumor. Fifteen patients had pathological schwannomas (neurinomas), 13 patients meningiomas, and 3 patients chordomas.

Oat leaf base: tissue with an efficient regeneration capacity.

An efficient short term regeneration system using seedling derived oat (Avena sativa) leaf tissue has been developed. Callus derived from the leaf base showed a higher response of plant regeneration than callus initiated from mesocotyls and more mature parts of the leaves. A correlation between the nuclear DNA content of the donor material, as analysed with flow cytometry, and its ability to form callus was observed.