Edge-substituents and center metal optimization boosting oxygen electrocatalysis in porphyrin-based covalent organic polymers.

The promising non-noble electrocatalyst with well-defined structure is significant for both oxygen reduction reaction (ORR) and oxygen evolution reaction (OER) for the renewable energy devices like zinc-air batteries (ZABs). Herein, the four phenyl-linked cobaltporphyrin-based covalent organic polymers (COPs-1-4) with the different edge substituents (1 = -tBu, 2 = -Me, 3 = -F, and 4 = -CF) are firstly designed and synthesized via a simple, efficient one-pot method. With the increase of electron donating capacity of the substituents, the highest occupied molecular orbital energy (E) gradually increases in the order of COP-4 < COP-3 < COP-2 < COP-1.

A chitosan macroporous hydrogel integrating enrichment, adsorption and delivery of blood clotting components for rapid hemostasis.

Traditional hemostatic hydrogels face considerable limitations in achieving rapid control of severe bleedings, a crucial factor in reducing casualties in both military and civilian settings. This study presents a chitosan-based hemostatic hydrogel with interconnected secondary macropores designed to enhance interactions with blood clotting components by reducing diffusion resistance and increasing contact area. The macropores were created using a straightforward process involving NaOH-mediated SiO template dissolution and NH generation.

Carbonic anhydrase encapsulation using bamboo cellulose scaffolds for efficient CO capture and conversion.

Utilizing carbonic anhydrase (CA) to catalyze CO hydration offers a sustainable and potent approach for carbon capture and utilization. To enhance CA's reusability and stability for successful industrial applications, enzyme immobilization is essential. In this study, delignified bamboo cellulose served as a renewable porous scaffold for immobilizing CA through oxidation-induced cellulose aldehydation followed by Schiff base linkage.

Thermo-assisted fabrication of a novel shape-memory hyaluronic acid sponge for non-compressible hemorrhage control.

Hyaluronic acid (HA), a major component of skin extracellular matrix, provides an excellent framework for hemostatic design; however, there still lacks HA materials tailored with superior mechanical properties to address non-compressible hemorrhages. Here, we present a solvent-free thermal approach for constructing a shape-memory HA sponge for this application. Following facile thermal incubation around 130 °C, HA underwent cross-linking via esterification with poly(acrylic acid) within the sponge pre-shaped through a prior freeze-drying process.

Crystalline metal phosphide-coated amorphous iron oxide-hydroxide (FeOOH) with oxygen vacancies as highly active and stable oxygen evolution catalyst in alkaline seawater at high current density.

In this study, we employed a straightforward phosphorylation approach to achieve a dual objective: constructing c-a heterostructures consisting of crystalline NiP and amorphous FeOOH, while simultaneously enhancing oxygen vacancies. The resulting oxygen evolution reaction (OER) catalyst, NiP/FeOOH/NF, exhibited remarkable performance with current densities of 500 mA cm in both 1 M KOH and 1 M KOH + seawater, requiring low overpotentials of only 288 and 365 mV, respectively. Furthermore, NiP/FeOOH/NF exhibited only a slight increase in overpotential, with increments of 18 mV and 70 mV in 1 M KOH after 15 and 150 h, and 32 mV and 108 mV in 1 M KOH + seawater at 500 mA cm after 15 and 150 h, respectively.

Chitosan thermogelation and cascade mineralization via sequential CaCO incorporations for wound care.

Physically crosslinked hydrogels have shown great potential as excellent and eco-friendly matrices for wound management. Herein, we demonstrate the development of a thermosensitive chitosan hydrogel system using CaCO as a gelling agent, followed by CaCO mineralization to fine-tune its properties. The chitosan hydrogel effectively gelled at 37 °C and above after an incubation period of at least 2 h, facilitated by the CaCO-mediated slow deprotonation of primary amine groups on chitosan polymers.

Development of alginate macroporous hydrogels using sacrificial CaCO particles for enhanced hemostasis.

Polymeric hydrogels have increasingly garnered attention in the field of hemostasis. However, there remains a lack of targeted development and evaluation of non-dense polymeric hydrogels with physically incorporated pores to enhance hemostasis. Here, we present a facile route to macroporous alginate hydrogels using acid-induced CaCO dissolution to provide Ca for alginate gelation and CO bubbles for subsequent macropore formation.

A peptide-engineered alginate aerogel with synergistic blood-absorbing and platelet-binding capabilities to rapidly stop bleeding.

Polysaccharide matrix infused with hemostasis-stimulating chemistry represents a critical medical need of bleeding management. Herein, we describe the development of a polysaccharide-peptide conjugate platform, an alginate engineered with fibrinogen-derived platelet-binding peptides (APE). The alginate backbone was found to allow for multivalent grafting of the peptides.

Trojan-horse mineralization of trigger factor to impregnate non-woven alginate fabrics for enhanced hemostatic efficacy.

Uncontrolled hemorrhage remains a leading cause of mortality after trauma. This work describes a facile mineralization strategy for enhancing hemostatic efficacy of alginate non-woven fabrics, involving the precipitation of amorphous CaCO induced by alginate fibers, along with Trojan-horse-like tissue factor (TF) encapsulation. The amorphous CaCO served as a transient carrier, capable of releasing Ca and TF upon contact with blood.

Armoring a liposome-integrated tissue factor with sacrificial CaCO to form potent self-propelled hemostats.

The development of hemostatic materials suitable for diverse emergency scenarios is of paramount significance, and there is growing interest in wound-site delivery of hemostasis-enhancing agents that can leverage the body's inherent mechanisms. Herein we report the design and performance of a biomimetic nanoparticle system enclosing tissue factor (TF), the most potent known blood coagulation trigger, which was reconstituted into liposomes and shielded by the liposome-templated CaCO mineralization. The mineral coatings, which mainly comprised water-soluble amorphous and vateritic phases, synergized with the lipidated TF to improve blood coagulation .

Atomically Dispersed Ni-N Sites Assist Pt Ni Nanocages with Pt Skin to Synergistically Enhance Oxygen Reduction Activity and Stability.

Currently, the rarity and high cost of platinum (Pt)-based electrocatalysts seriously limit their commercial application in fuel cells cathode. Decorating Pt with atomically dispersed metal-nitrogen sites possibly offers an effective pathway to synergy tailor their catalytic activity and stability. Here active and stable oxygen reduction reaction (ORR) electrocatalysts (Pt Ni@Ni-N -C) by in situ loading Pt Ni nanocages with Pt skin on single-atom nickel-nitrogen (Ni-N ) embedded carbon supports are designed and constructed.

Sprayable surface-adaptive biocompatible membranes for efficient hemostasis via assembly of chitosan and polyphosphate.

This work describes a hemostatic membrane system (or surface coating) based on spray-assisted layer-by-layer electrostatic assemblies of oppositely charged polyphosphate (polyP) and chitosan (Cs). The as-prepared membrane formed a robust micro-stratified porous structure with high flexibility. Both blood clotting test and rodent hepatic severe hemorrhage model revealed the excellent hemostatic performance of the membrane system, benefitting from the robust assembly and synergistic effect of polyP/Cs as well as membrane surface chemistry.

Incorporation of tissue factor-integrated liposome and silica nanoparticle into collagen hydrogel as a promising hemostatic system.

Bleeding complications are associated with substantial tissue morbidities and mortalities. Biomimetic composite materials that possess the ability to sufficiently stimulate and augment different physiological mechanisms of hemostasis are highly desirable to reduce bleeding-related casualties, which, however, are still largely under-explored. This study aims to develop a composite hemostatic system by combining collagen hydrogel with tissue factor (TF)-integrated liposome and silica nanoparticle, which could integrate the platelet plug-promoting capacity of collagen with the abilities of the latter two components to activate the extrinsic and intrinsic pathways of coagulation respectively.

Injectable thermogelling bioadhesive chitosan-based hydrogels for efficient hemostasis.

Development of an injectable hemostatic for treating noncompressible or irregularly shaped bleeding wounds remains a pressing medical need. Herein, we report an injectable thermogelling chitosan/glycerophosphate formulation that enhances gel-forming capacity and wet tissue adherence by incorporation of dihydrocaffeic acid (DHCA). This was found to decrease gelation time by >2 times around 37 °C while increasing hydrogel internal network structure, with its tissue adhesive strength >2 times greater than that of the non-composite hydrogel or previously reported.

Protein Nanobarrel for Integrating Chlorophyll Molecules and Its Photochemical Performance.

Taking inspiration from biology's effectiveness in nanoscale organization of chlorophylls for photosynthesis, we describe here a design for chlorophyll-protein conjugates that exploits the central hydrophobic cavity of GroEL protein nanobarrel as a binding pocket for chlorophyll. We found water-soluble conjugates of chlorophyll with GroEL could be easily generated detergent dialysis. The number of chlorophyll units bound to GroEL is tunable by varying the equilibrium concentration of chlorophyll during dialysis.

Interspecies Phylogenetic Analysis of in High-incidence Areas of Hunan Province, China.

Objective: This study aims to investigate the infection of ( ) in high-incidence areas of Hunan Province, China. The phylogenetic analysis of the species in the highly infected areas was carried out.

Method: Infection of the definitive human host and intermediate fish host by was investigated, and the mitochondrial genes and were used as genetic markers for phylogenetic analysis.

Tissue factor-loaded collagen/alginate hydrogel beads as a hemostatic agent.

Uncontrolled hemorrhage accounts for a significant proportion of annual mortality worldwide. The development of bioinspired hemostatic composites can effectively reduce hemorrhage and related deaths. This work aims to develop an efficient hemostatic agent by incorporating tissue factor (TF) integrated liposomes and collagen, which are capable of augmenting different inherent hemostatic mechanisms, into hemostasis-stimulating alginate matrix.

Preparation and characterization of tissue-factor-loaded alginate: Toward a bioactive hemostatic material.

Tissue factor (TF), an integral membrane protein, is by far the most potent known triggering agent of blood coagulation. Inspired by TF's effectiveness in initiating coagulation, this work aims to develop hemostatic materials with TF-integrated liposomes, which combined with alginate biopolymers are designed as composite pastes or hydrogels cross-linked with Ca. Fluorescence measurements revealed that the proteoliposomes were evenly distributed within alginate matrices, which also remained intact after release into simulated body fluid.

Tubeimoside‑1 induces apoptosis in human glioma U251 cells by suppressing PI3K/Akt‑mediated signaling pathways.

Tubeimoside-1 (TBMS1), a traditional Chinese herb extracted from Bolbostemma paniculatum (Maxim.), induces apoptosis in a number of human cancer cell lines. TBMS1 has been reported to induce apoptosis in human glioma cells, however the mechanism remains to be elucidated.

Nanoscale integration of porphyrin in GroEL protein cage: Photophysical and photochemical investigation.

In this paper, we introduce a new type of functional, supramolecular porphyrin conjugate created using the bacterial GroEL protein cage based on non-specific hydrophobic interaction. The synthesis, structure and property of the porphyrin conjugate were characterized by dynamic light scattering, UV-vis spectroscopy and fluorescence spectroscopy. We observed that the model zinc-tetraphenylporphyrin (Zn-TPP) with high hydrophobicity can be well-dispersed in aqueous solutions with the aid of GroEL open chamber, which is known to be a favorable nanocompartment for aggregation-prone molecules.

Fe(III)EDTA and Fe(II)EDTA-NO reduction by a sulfate reducing bacterium in NO and SO₂ scrubbing liquor.

A viable process concept, based on NO and SO2 absorption into an alkaline Fe(II)EDTA (EDTA: ethylenediaminetetraacetic acid) solution in a scrubber combined with biological reduction of the absorbed SO2 utilizing sulfate reducing bacteria (SRB) and regeneration of the scrubbing liquor in a single bioreactor, was developed. The SRB, Desulfovibrio sp. CMX, was used and its sulfate reduction performances in FeEDTA solutions and Fe(II)EDTA-NO had been investigated.

Evaluation of simultaneous nitrification and denitrification under controlled conditions by an aerobic denitrifier culture.

Paracoccus versutus LYM was characterized with the ability of simultaneous nitrification and denitrification (SND) in presence of NH4(+)-N and NO3(-)-N (140mg/L-N each). However, there was approximate 95.80mg/L NO2(-)-N left during SND process.

Management of lymph node metastases from an unknown primary site to the head and neck (Review).

Cancer of unknown primary site (CUP) is an intriguing clinical phenomenon found in ~3-9% of all head and neck cancers. It has not yet been determined whether CUP forms a distinct biological entity with specific genetic and phenotypic characteristics, or whether it is the clinical presentation of metastasis in patients with an undetected primary tumor and no visible clinical signs. The treatment of patients with cervical lymph node metastases from CUP remains controversial, due to the lack of randomized clinical trials comparing different treatment options.