Publications by authors named "Zhu-Mei Cui"
Objective: To analyze the management and prognosis of primary choriocarcinoma (PCC) in male patients.
Methods: The clinical records of males with PCC who were treated at Peking Union Medical College Hospital between 1990 and 2012 were analyzed retrospectively. The literature regarding this clinical condition was also reviewed.
[MicroRNA-16 regulates the proliferation, invasion and apoptosis of ovarian epithelial carcinoma cells in vitro].
Zhonghua Fu Chan Ke Za Zhi
November 2012
Objective: To study the role and mechanism of microRNA-16 (miR-16) in the proliferation, invasion and apoptosis of ovarian epithelial carcinoma cells in vitro.
Methods: The SKOV-3 cells were transfected with miR-16 mimics or negative control RNA (NC) by lipofectamine 2000. The expression of miR-16 was detected by real-time reverse transcription (RT)-PCR in SKOV-3 cells, and western blot was used to detect the expression of vascular endothelial growth factor (VEGF), matrix metalloproteinase-2 (MMP-2) and bcl-2 protein.
The chemokine CXCL12 is highly expressed in gynecologic tumors and is widely known to play a biologically relevant role in tumor growth and spread. Recent evidence suggests that CXCL16, a novel chemokine, is overexpressed in inflammation-associated tumors and mediates pro-tumorigenic effects of inflammation in prostate cancer. We therefore analyzed the expression of CXCL12 and CXCL16 and their respective receptors CXCR4 and CXCR6 in cervical intraepithelial neoplasia (CIN) and cervical cancer and further assessed their association with clinicopathologic features and outcomes.
View Article and Find Full Text PDF[Summary of "the Forth National Academic Conference on Endometriosis and Chronic Pelvic Pain"].
Zhonghua Fu Chan Ke Za Zhi
November 2011
[Role of microRNA-21 in the proliferation and apoptosis of ovarian epithelial carcinoma cells].
Zhonghua Fu Chan Ke Za Zhi
September 2011
Article Synopsis
- This study aims to understand how microRNA-21 (miR-21) affects the growth and programmed cell death (apoptosis) of ovarian epithelial cancer cells.
- Researchers created a specific RNA to target miR-21 and compared three groups of cells: one with the RNA, one with a negative control, and one with no treatment.
- Results showed that the group with the targeted RNA had lower levels of miR-21 and higher levels of a protein that promotes apoptosis, resulting in more cell death compared to the control groups.
Recent evidence suggests that the chemokine axis of CXC chemokine ligand-12 and its receptor CXC chemokine receptor-4 (CXCL12/CXCR4) is highly expressed in gynecological tumors and the axis of CXC chemokine ligand-16 and CXC chemokine receptor-6 (CXCL16/CXCR6) is overexpressed in inflammation-associated tumors. This study aimed to determine the relationship between CXCL12/CXCR4, CXCL16/CXCR6 and ovarian carcinoma's clinicopathologic features and prognosis. Accordingly, the expression of these proteins in ovarian tissues was detected by tissue microarray and immunohistochemistry.
View Article and Find Full Text PDF[Effect of small interference RNA on E6, E7 mRNA of human papillomavirus type-18 in cervical cancer cells].
Zhonghua Fu Chan Ke Za Zhi
March 2006
Objective: To study the effect of small interference RNA on E6, E7 mRNA of human papillomavirus type-18 (HPV18) in cervical cancer cells.
Methods: The specific HPV18E6 and E7 siRNA synthesized by in virtro transcription. The cell activity was detected by methyl thiazolyl tetrazolium (MTT) assay to determine the most suitable concentration, then cells were transfected into HPV18 cervical cancer cells by oligofectamine.
[Expression of SLP-2 mRNA in endometrial cancer and its significance].
Zhonghua Fu Chan Ke Za Zhi
August 2005
Objective: To characterize the differential expression of SLP-2 in endometrial cancer, and to study the effect of human SLP-2 gene on human endometrial cancer cell line.
Methods: The expression of SLP-2 gene in 32 cases of endometrial cancer and 28 cases of normal endometrial tissues was examined by semi-quantitative RT-PCR. Eukaryotic expression vectors of sense and antisense SLP-2 were constructed and transfected into HEC-1B cell line using lipofectamine 2000 respectively.
Objective: To investigate in vivo reversal of multidrug resistance and biological properties of a drug resistant cell line of choriocarcinoma transduced by human tumor necrosis factor-alpha (TNF-alpha) gene via the establishment of its animal model.
Methods: Choriocarcinoma cell line JEG-3, drug-resistant choriocarcinoma cell line JEG-3/VP2, and human TNF-alpha-transduced drug-resistant choriocarcinoma cell line JEG-3/VP2/TNF-alpha were injected subcutaneously in the neck of nude mices. Tumor size and weight were routinely measured, tumor histological structure was observed and its chemosensitivity was tested.