Exogenous allyl isothiocyanate mitigates the drought stress by regulating the stomatal characteristic, antioxidant capacity, and glucosinolate metabolism in pakchoi.

Drought is a global issue that has increasingly garnered worldwide attention. Glucosinolates (GSLs) are significant sulfur-containing compounds in cruciferous plants such as pakchoi (Brassica rapa L. ssp.

Transcriptomic analysis of succulent stem development of Chinese kale ( var. Bailey) and its synthetic allotetraploid RNA sequencing.

Chinese kale ( var. Bailey, CC) is a succulent stem vegetable in the family. Its allotetraploid (AACC) vegetable germplasm, which was synthesized distant hybridization with the colloquially named 'yellow turnip' ( L.

Effect of short-term high temperature on the accumulation of glucosinolates in Brassica rapa.

Glucosinolates, an important class of secondary metabolites in cruciferous vegetables, play a crucial role in protecting plants from stress-related damage. The mechanism of glucosinolate synthesis under short-term high temperature stress has not been sufficiently studied. In this work, we investigated the changes in transcription factors, synthetic genes, and related metabolites involved in glucosinolate synthesis by pakchoi seedlings under short-term high temperature stress (40 °C for 8 h).

One-Step Bioconversion of Fatty Acids into C8-C9 Volatile Aroma Compounds by a Multifunctional Lipoxygenase Cloned from Pyropia haitanensis.

The multifunctional lipoxygenase PhLOX cloned from Pyropia haitanensis was expressed in Escherichia coli with 24.4 mg·L yield. PhLOX could catalyze the one-step bioconversion of C18-C22 fatty acids into C8-C9 volatile organic compounds (VOCs), displaying higher catalytic efficiency for eicosenoic and docosenoic acids than for octadecenoic acids.

Leaf and root glucosinolate profiles of Chinese cabbage (Brassica rapa ssp. pekinensis) as a systemic response to methyl jasmonate and salicylic acid elicitation.

Glucosinolates (GSs) are an important group of defensive phytochemicals mainly found in Brassicaceae. Plant hormones jasmonic acid (JA) and salicylic acid (SA) are major regulators of plant response to pathogen attack. However, there is little information about the interactive effect of both elicitors on inducing GS biosynthesis in Chinese cabbage (Brassica rapa ssp.

A tomato endoplasmic reticulum (ER)-type omega-3 fatty acid desaturase (LeFAD3) functions in early seedling tolerance to salinity stress.

Transgenic tomato plants overexpressing LeFAD3 sense and antisense sequences were generated. Salt stress suppressed the growth of WT and antisense plants to a higher extent than that in sense plants. In this study, we investigated the role of the LeFAD3-encoding ER-type omega-3 fatty acid desaturase in salt tolerance in tomato plants.

Variation in glucosinolates in pak choi cultivars and various organs at different stages of vegetative growth during the harvest period.

Glucosinolates (GSs) play an important role in plant defense systems and human nutrition. We investigated the content and composition of GSs in the shoots and roots of seven cultivars of pak choi. We found that 'Si Yue Man' had the highest total and aliphatic GS contents in the shoots and the highest benzenic GS content in the roots, 'Shanghai Qing' contained the highest amounts of benzenic and total GS contents in the roots, while 'Nanjing Zhong Gan Bai' had the lowest benzenic, indole, and total GS contents in both the shoots and roots.

Antisense-mediated depletion of GMPase gene expression in tobacco decreases plant tolerance to temperature stresses and alters plant development.

In our previous work [1] we investigated the role of tomato GDP-mannose pyrophosphorylase (EC 2.7.7.

An assessment of psychological noise reduction by landscape plants.

The emphasis in the term 'Green Transportation' is on the word 'green'. Green transportation focuses on the construction of a slow transport system with a visually pleasing, easy and secure trip environment composed of urban parks, green roadside spaces and some other space that is full of landscape plants. This trip environment encourages residents to make trip choices that reduce fuel consumption and pollution and is one of the most important ways of popularizing green transportation.

Effects of osmotic stress on antioxidant enzymes activities in leaf discs of PSAG12-IPT modified Gerbera.

Leaf senescence is often caused by water deficit and the chimeric gene P(SAG12)-IPT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance (TBARS) and antioxidant enzymes activities were investigated during leaf senescence of P(SAGl2)-IPT modified gerbera induced by osmotic stress compared with the control plant (wild type). Leaf discs were incubated in 20%, 40% (w/v) polyethylene glycol (PEG) 6000 nutrient solution for 20 h under continuous light [130 micromol/(m(2) x s)].

[Effects of high concentration manganese on active oxygen production and antioxidant enzyme activities in cucumber leaves under different irradiations].

Effects of high concentration manganese on active oxygen production and antioxidant enzymes in cucumber leaves under natural irradiation and 1/2 natural irradiation were studied. High concentration manganese increased the H2O2 content and O2*- producing rate of cucumber leaves (Fig.1A, B) and caused lipid peroxidation (Fig.

[Primary targeting of functional regions involved in transcriptional regulation on watermelon fruit-specific promoter WSP].

Fruit ripening is associated with a number of physiological and biochemical changes. They include degradation of chlorophyll, synthesis of flavor compounds, carotenoid biosynthesis, conversion of starch to sugars, cell wall solublisation and fruit softening. These changes are brought about by the expression of specific genes.

[Effects of iso-osmotic salt stress on the activities of antioxidative enzymes, H+-ATPase and H+-PPase in tomato plants].

Seedlings of tomato (Lycopersicon esculentum Mill.) were grown in nutrient solution to investigate the changes in malondialdehyde (MDA) and proline content, and activities of antioxidative enzymes, plasmalemma H(+)-ATPase, and tonoplast H(+)-ATPase and H(+)-PPase to iso-osmotic stress of Ca(NO(3))(2) (80 mmol/L) and NaCl (120 mmol/L). The results indicated that both Ca(NO(3))(2) and NaCl stress significantly increased superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activities in leaf cytosols and chloroplasts.

Genotypic differences in effects of cadmium exposure on plant growth and contents of cadmium and elements in 14 cultivars of bai cai.

Fourteen cultivars of bai cai (Brassica campestris L. ssp. chinensis var.

[Transformation of Bt-CpTi fusion protein gene to cabbage (Brassica oleracea var. capitata) mediated by Agrobacterium tumefaciens and particle bombardment].

Bt-CpTI fusion protein gene was transferred to the explants of hypocotyl, cotyledon with petiole and shoot apex of cabbage (Brassica oleracea L. var. capitata) variety "Zhonggan No 8" via Agrobacterium tumefaciens and particle bombardment, and 13 kanamycin-resistant plants were obtained.

[Transformation of wml1 5' promoter region into tomato plants and studies on its transcriptional regulation role].

According to its restriction sites, fragments of 1573 bp, 1197 bp, 896 bp and 795 bp were obtained from the 5' promoter region of wml1 and fused with the coding sequence of the GUS gene. Constructs containing these fragments were introduced into tomato plants via Agrobacterium-mediated transformation. Histochemical assay of GUS expression in transgenic tomato plants revealed that fragments of 1573 bp, 1197 bp, 896 bp were able to direct GUS expression in fruits of 15, 30, 45 days after anthesis with the expression level of GUS increasing with fruit development, but not in leaves, stems and roots.

Expression of CycD3 is transiently increased by pollination and N-(2-chloro-4-pyridyl)-N'-phenylurea in ovaries of Lagenaria leucantha.

Lagenaria leucantha is an important vegetable crop and a potential model for the study of fruit development. To study the function of D cyclins in fruit development, full-length cDNA clones for two D cyclin genes were isolated from young ovaries of Lagenaria leucantha. They were classified as D3 cyclins by sequence similarities and phylogenetic analysis, and nominated LlCycD3;1 and LlCycD3;2, respectively.