Inotodiol induces hepatocellular carcinoma apoptosis by activation of MAPK/ERK pathway.

Hepatocellular carcinoma(HCC) has a high mortality and morbidity rate and seriously jeopardizes human life. Chemicals and chemotherapeutic agents have been experiencing problems such as side effects and drug resistance in the treatment of HCC, which cannot meet the needs of clinical treatment. Therefore, finding novel low-toxicity and high-efficiency anti-hepatocellular carcinoma drugs and exploring their mechanisms of action have become the current problems to be solved in the treatment of HCC.

Geometry-Based Synchrosqueezing S-Transform with Shifted Instantaneous Frequency Estimator Applied to Gearbox Fault Diagnosis.

This paper introduces a novel geometry-based synchrosqueezing S-transform (GSSST) for advanced gearbox fault diagnosis, designed to enhance diagnostic precision in both planetary and parallel gearboxes. Traditional time-frequency analysis (TFA) methods, such as the Synchrosqueezing S-transform (SSST), often face challenges in accurately representing fault-related features when significant mode closely spaced components are present. The proposed GSSST method overcomes these limitations by implementing an intuitive geometric reassignment framework, which reassigns time-frequency (TF) coefficients to maximize energy concentration, thereby allowing fault components to be distinctly isolated even under challenging conditions.

Dietary Tannic Acid Promotes Growth Performance and Resistance Against Infection by Improving the Antioxidative Capacity and Intestinal Health in the Chinese Soft-Shelled Turtle ().

To investigate the effect of tannic acid (TA) on the growth, disease resistance, and intestinal health of Chinese soft-shelled turtles, individual turtles were fed with 0 g/kg (CG), 0.5 g/kg, 1 g/kg, 2 g/kg, and 4 g/kg TA diets for 98 days. Afterwards, the turtles' disease resistance was tested using .

Biochemical, Histological, and Multi-Omics Analyses Reveal the Molecular and Metabolic Mechanisms of Cold Stress Response in the Chinese Soft-Shelled Turtle ().

The Chinese soft-shelled turtle (), a type of warm-water reptile, is frequently chosen as the model animal to understand how organisms respond to environmental stressors. However, the responsive mechanism of to natural cold stress is unclear, especially in terms of metabolic pattern and molecular pathways. Herein, plasma biochemical, hepatic morphological, apoptotic, transcriptomic, and metabolomic detection methods were performed to investigate the response of to acute cold stress.

Trajectory Planning for Unmanned Vehicles on Airport Apron Under Aircraft-Vehicle-Airfield Collaboration.

To address the issue of safe, orderly, and efficient operation for unmanned vehicles within the apron area in the future, a hardware framework of aircraft-vehicle-airfield collaboration and a trajectory planning method for unmanned vehicles on the apron were proposed. As for the vehicle-airfield perspective, a collaboration mechanism between flight support tasks and unmanned vehicle departure movement was constructed. As for the latter, a control mechanism was established for the right-of-way control of the apron.

Impact of Different Land-Use Types on Soil Microbial Carbon Metabolism Function in Arid Region of Alpine Grassland.

There are discrepancies that exist in the effects of different land uses on soil organic carbon (SOC) and soil microbial carbon metabolism functions. However, the impact of land-use type changes on soil microbial carbon metabolism in alpine grassland arid areas is not well understood, hindering our understanding of the carbon cycling processes in these ecosystems. Therefore, we chose three types of land use (continuous reclamation of grassland (RG), abandoned grassland (AG), and natural grazing grassland (GG)) to study the microbial carbon metabolism and its driving factors by the Biolog-ECO method.

Optimal CO intake in metastable water film in mesoporous materials.

The feasibility of carbon mineralization relies on the carbonation efficiency of CO-reactive minerals, which is largely governed by the water content and state within material mesopores. Yet, the pivotal role of confined water in regulating carbonation efficiency at the nanoscale is not well understood. Here, we show that the maximum CO intake occurs at an optimal relative humidity (RH) when capillary condensation initiates within the hydrophilic mesopores.

Sex-Dimorphic Differential Expression Profiles in the Brain of the Adult Chinese Soft-Shelled Turtle, .

The Chinese soft-shelled turtle () is an economically important species in aquaculture, and its growth pattern is characterized by significant sexual dimorphism. However, the underlying molecular mechanisms of this phenomenon have mostly been investigated in the gonadal tissues of , and there are no articles on sex differentiation from the brain of . Here, we analyzed transcriptomes of the brains of adult male and female using high-throughput Illumina sequencing technology, establishing a set of differential genes and differential transcription factors.

Chromosome-level genome assembly of the mud carp (Cirrhinus molitorella) using PacBio HiFi and Hi-C sequencing.

The mud carp (Cirrhinus molitorella) is an important economic farmed fish, mainly distributed in South China and Southeast Asia due to its strong adaptability and high yield. Despite its economic importance, the paucity of genomic information has constrained detailed genetic research and breeding efforts. In this study, we utilized PacBio HiFi long-read sequencing and Hi-C technologies to generate a meticulously assembled chromosome-level genome of the mud carp.

Collective molecular-scale carbonation path in aqueous solutions with sufficient structural sampling: From CO2 to CaCO3.

The carbonation reaction is essential in the global carbon cycle and in the carbon dioxide (CO2) capture. In oceans (pH 8.1) or in synthetic materials such as cement or geopolymers (pH over 12), the basic pH conditions affect the reaction rate of carbonation.

PD-1 deficiency impairs eosinophil recruitment to tissue during Trichinella spiralis infection.

Blockade of programmed cell death 1 (PD-1) is considered a promising strategy for controlling pathogen infection by enhancing host immune cell function. Eosinophils, which play a crucial role in type 2 immune responses, are essential components of the host defense against helminth infection. Here, we investigate the role of PD-1 in eosinophilia during Trichinella spiralis infection in mice.

Liquidambaric acid inhibits the proliferation of hepatocellular carcinoma cells by targeting PPARα-RXRα to down-regulate fatty acid metabolism.

Hepatocellular carcinoma (HCC) is a primary malignant tumor of the liver. As the global obesity rate rises, non-alcoholic fatty liver disease (NAFLD) has emerged as the most rapidly increasing cause of HCC. Consequently, the regulation of lipid metabolism has become a crucial target for the prevention and treatment of HCC.

Spinosad blocks CHRNA5 mediated EGFR signaling pathway activation to inhibit lung adenocarcinoma proliferation.

Lung adenocarcinoma (LUAD) is the leading cause of cancer death worldwide, with high incidence and low survival rates. Nicotinic acetylcholine receptors play an important role in the progression of LUAD. In this study, a screening of 17 nicotinic acetylcholine receptor allosteric agents revealed that spinosad effectively suppressed the proliferation of LUAD cells.

Paramyosin Alleviates Collagen-Induced Arthritis in Mice by Modulating CD4 T Cell Differentiation.

Rheumatoid arthritis (RA) is an autoimmune disease that significantly impacts quality of life by disrupting CD4 T cell immune homeostasis. The identification of a low-side-effect drug for RA treatment is urgently needed. Our previous study suggests that paramyosin (-Pmy) has immunomodulatory effects, but its potential effect on CD4 T cell response in RA remains unclear.

PD-1/PD-L1 Provides Protective Role in Hypoxia-Induced Pulmonary Vascular Remodeling.

Background: Hypoxia-induced pulmonary hypertension (HPH) is a T helper 17 cell response-driven disease, and PD-1 (programmed cell death 1)/PD-L1 (programmed cell death-ligand 1) inhibitor-associated pulmonary hypertension has been reported recently. This study is designed to explore whether the PD-1/PD-L1 pathway participates in HPH via regulating endothelial dysfunction and T helper 17 cell response.

Methods: Lung tissue samples were obtained from eligible patients.

Integrated time-series biochemical, transcriptomic, and metabolomic analyses reveal key metabolites and signaling pathways in the liver of the Chinese soft-shelled turtle () against infection.

Introduction: , a bacterium widely distributed in the natural environment, causes multiple diseases in various animals. Exploring the mechanism of the host defense against can help develop efficient strategies against infection.

Methods: Herein, we investigated the temporal influence of A.

Crystal structure of calreticulin and the structural basis of its complement evasion mechanism involving C1q.

Helminths produce calreticulin (CRT) to immunomodulate the host immune system as a survival strategy. However, the structure of helminth-derived CRT and the structural basis of the immune evasion process remains unclarified. Previous study found that the tissue-dwelling helminth produces calreticulin (TsCRT), which binds C1q to inhibit activation of the complement classical pathway.

Screening of temperature-responsive signalling molecules during sex differentiation in Asian yellow pond turtle (Mauremys mutica).

Background: The Asian yellow pond turtle (Mauremys mutica) is an important commercial freshwater aquaculture species in China. This species is a highly sexually dimorphic species, with males growing at a faster rate than females and exhibits temperature-dependent sex determination (TSD), in which the incubation temperature during embryonic development determines the sexual fate. However, the mechanisms of the sex determination or sex differentiation in the Asian yellow pond turtle are remain a mystery.

Whole-Genome Identification and Characterization of the DKK Gene Family and Its Transcription Profiles: An Analysis of the Chinese Soft-Shell Turtle ().

The DKK family is a canonical small family of WNT antagonists. Though recent studies have suggested that the DKK gene family may be involved in sex differentiation in , there are still a lot of things about the DKK gene family that we do not know. In this study, we used bioinformatics methods to identify members of the DKK gene family in and analyzed their phylogeny, covariance, gene structure, structural domains, promoter conserved sites, signal peptides, gonadal transcription factors, transcriptional profiles, and tissue expression profiles.

Dihydroartemisinin, a potential PTGS1 inhibitor, potentiated cisplatin-induced cell death in non-small cell lung cancer through activating ROS-mediated multiple signaling pathways.

Dihydroartemisinin (DHA) exerts an anti-tumor effect in multiple cancers, however, the molecular mechanism of DHA and whether DHA facilitates the anti-tumor efficacy of cisplatin in non-small cell lung cancer (NSCLC) are unclear. Here, we found that DHA potentiated the anti-tumor effects of cisplatin in NSCLC cells by stimulating reactive oxygen species (ROS)-mediated endoplasmic reticulum (ER) stress, C-Jun-amino-terminal kinase (JNK) and p38 MAPK signaling pathways both in vitro and in vivo. Of note, we demonstrated for the first time that DHA inhibits prostaglandin G/H synthase 1 (PTGS1) expression, resulting in enhanced ROS production.