A homozygous loss-of-function mutation in CEP250 is associated with acephalic spermatozoa syndrome in humans.

Background: The presence of predominantly headless sperm in semen is a hallmark of acephalic spermatozoa syndrome, which is primarily caused by gene mutations in humans.

Purpose: To identify genetic causes for acephalic spermatozoa syndrome.

Methods: Polymerase chain reaction and Sanger sequencing were performed to define mutations in SUN5 and PMFBP1.

Apelin (APLN) is a biomarker contributing to the diagnosis and prognosis of hepatocellular carcinoma.

Liver cancer, classified as a malignant hepatic tumor, can be divided into two categories: primary, originating within the liver, and secondary, resulting from metastasis to the liver from other organs. Hepatocellular carcinoma (HCC) is the main form of primary liver cancer and the third leading cause of cancer-related deaths. The diagnosis and prognosis of HCC using current methods still face numerous challenges.

Rice seed storability: From molecular mechanisms to agricultural practices.

The storability of rice seeds is crucial for ensuring flexible planting options, agricultural seed security, and global food safety. With the intensification of global climate change and the constant fluctuations in agricultural production conditions, enhancing the storability of rice seeds has become particularly important. Seed storability is a complex quantitative trait regulated by both genetic and environmental factors.

Classification of pain expression images in elderly with hip fractures based on improved ResNet50 network.

The aim of this study is designed an improved ResNet 50 network to achieve automatic classification model for pain expressions by elderly patients with hip fractures. This study built a dataset by combining the advantages of deep learning in image recognition, using a hybrid of the Multi-Task Cascaded Convolutional Neural Networks (MTCNN). Based on ResNet50 network framework utilized transfer learning to implement model function.

Silencing LINC00987 ameliorates adriamycin resistance of acute myeloid leukemia via miR-4458/HMGA2 axis.

Background: Most patients with acute myeloid leukemia (AML) eventually develop drug resistance, leading to a poor prognosis. Dysregulated long gene non coding RNAs (lincRNAs) have been implicated in chemoresistance in AML. Unfortunately, the effects of lincRNAs which participate in regulating the Adriamycin (ADR) resistance in AML cells remain unclear.

Accurate and affordable detection of rifampicin and isoniazid resistance in Tuberculosis sputum specimens by multiplex PCR-multiple probes melting analysis.

Background: Escalating cases of multidrug-resistant tuberculosis (MDR-TB) pose a major challenge to global TB control efforts, necessitating innovative diagnostics to empower decentralized detection of gene mutations associated with resistance to rifampicin (RIF) and isoniazid (INH) in Mycobacterium tuberculosis (M. tuberculosis) in resource-constrained settings.

Methods: Combining multiplex fluorescent PCR and Multiple Probes Melting Analysis, we identified mutations in the rpoB, katG, ahpC and inhA genes from sputum specimens.

Regulatory mechanism and molecular genetic dissection of rice ( L.) grain size.

With the sharp increase of the global population, adequate food supply is a great challenge. Grain size is an essential determinant of rice yield and quality. It is a typical quantitative trait controlled by multiple genes.

Comparative physiological and transcriptome analysis between potassium-deficiency tolerant and sensitive sweetpotato genotypes in response to potassium-deficiency stress.

Background: Sweetpotato is a typical ''potassium (K) favoring'' food crop, which root differentiation process needs a large supply of potassium fertilizer and determine the final root yield. To further understand the regulatory network of the response to low potassium stress, here we analyze physiological and biochemical characteristics, and investigated root transcriptional changes in two sweetpotato genotypes, namely, - K tolerant "Xu32" and - K susceptible"NZ1".

Result: We found Xu32 had the higher capability of K absorption than NZ1 with better growth performance, higher net photosynthetic rate and higher chlorophyll contents under low potassium stress, and identified 889 differentially expressed genes (DEGs) in Xu32, 634 DEGs in NZ1, 256 common DEGs in both Xu32 and NZ1.

Rice Seed Protrusion Quantitative Trait Loci Mapping through Genome-Wide Association Study.

The germination of seeds is a prerequisite for crop production. Protrusion is important for seed germination, and visible radicle protrusion through seed covering layers is the second phase of the process of seed germination. Analyzing the mechanism of protrusion is important for the cultivation of rice varieties.

Correction: The impact of digital inclusive finance on SMEs' technological innovation activities-Empirical analysis based on the data of new third board enterprises.

[This corrects the article DOI: 10.1371/journal.pone.

Multi-attribute group decision-making based on Pythagorean fuzzy rough Aczel-Alsina aggregation operators and its applications to Medical diagnosis.

The fusion of information is a very hectic process whenever we analyze the information. Several frameworks have been introduced to reduce the uncertainty while fusing the information. Among those techniques, the Pythagorean fuzzy rough set (PyFRS), which is based on approximations is a key idea for dealing with uncertainty when data is taken from real-world circumstances.

How to promote knowledge transfer within R&D team? An evolutionary game based on prospect theory.

Knowledge transfer is the basis for R&D teams and enterprises to improve innovation performance, win market competition and seek sustainable development. In order to explore the path to promote knowledge transfer within the R&D team, this study considers the bounded rationality and risk preference of individuals, incorporates prospect theory into evolutionary game, constructs a perceived benefits matrix distinct from the traditional benefits matrix, and simulates the evolutionary game process. The results show that, R&D personnel's knowledge transfer decisions depend on the net income difference among strategies; only if perceived cost is less than the sum of perceived synergy benefit, perceived organization reward value, and perceived organization punishment value, can knowledge be fully shared and transferred within the R&D team.

Effect of physical exercise on sleep quality in college students: Mediating role of smartphone use.

Objective: To investigate the effect of physical exercise on sleep quality and the mediating effect of smartphone use behavior in college students.

Methods: A cross-sectional study design was adopted. An online survey of 5,075 college students was conducted using the Physical Activity Rating Scale-3, the Pittsburgh Sleep Quality Index, and the Mobile Phone Addiction Tendency Scale.

The impact of digital inclusive finance on SMEs' technological innovation activities-Empirical analysis based on the data of new third board enterprises.

The landscape of financial technology is undergoing a continuous evolution, driven by the relentless advancement of information technology. In this transformative milieu, digital finance has emerged as a novel financial paradigm, offering a blueprint for fostering inclusive finance. With a particular emphasis on its implications for Small and Medium-sized Enterprises (SMEs), this article harnesses a comprehensive dataset spanning the years 2011 to 2021, encompassing digital inclusive finance and SMEs listed on the SME board.

Spatial-temporal coupling coordination and interaction between digitalization and traditional industrial upgrading: a case study of the Yellow River Basin.

The realization of coupling coordination between digitalization and traditional industrial upgrading in the Yellow River Basin holds significant practical value for promoting high-quality industrial development in the region. In order to assess this coupling coordination, we utilized inter-provincial panel data from nine provinces in the Yellow River Basin, covering the period from 2011 to 2020. Through the application of a coupling coordination degree model, we calculated the degree of coupling coordination and relative development between digitalization and traditional industrial upgrading.

Asymmetric nexus between pandemic uncertainty and public health spendings: Evidence from quantile estimation.

The COVID-19 pandemic has brought significant challenges to healthcare systems worldwide, prompting governments to allocate substantial resources toward public health spendings (PHS). However, the uncertainties surrounding the pandemic have raised questions about the effectiveness and sustainability of such expenditures. This research analyzes the nonlinear link between pandemic uncertainty (PNU) and PHS in countries with highest PNU (USA, India, France, Germany, UK, Saudi Arabia, South Korea, Indonesia, Japan, and China).

Genome-Wide Analysis of the PHT Gene Family and Its Response to Mycorrhizal Symbiosis in Tomatoes under Phosphate Starvation Conditions.

Phosphate is one of the essential mineral nutrients. Phosphate transporter genes (PHTs) play an important role in Pi acquisition and homeostasis in tomato plants. However, basic biological information on PHT genes and their responses of symbiosis with arbuscular mycorrhizal in the genome remains largely unknown.

Cross-domain Nuclei Detection in Histopathology Images using Graph-based Nuclei Feature Alignment.

As powerful tools deep neural networks have been successfully adopted for nuclei detection in histopathology images, whereas require the same probability distribution between training and testing data. However, domain shift among histopathology images widely exists in real-world applications and severely deteriorates the detection performance of deep neural networks. Despite encouraging results of existing domain adaptation methods, there remain challenges for cross-domain nuclei detection task.

A theoretical model of factors influencing online consumer purchasing behavior through electronic word of mouth data mining and analysis.

The coronavirus disease 2019 pandemic has impacted and changed consumer behavior because of a prolonged quarantine and lockdown. This study proposed a theoretical framework to explore and define the influencing factors of online consumer purchasing behavior (OCPB) based on electronic word-of-mouth (e-WOM) data mining and analysis. Data pertaining to e-WOM were crawled from smartphone product reviews from the two most popular online shopping platforms in China, Jingdong.

Immune-enhancing activity of polysaccharides and flavonoids derived from YASH1.

(Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement. This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from () and to provide a theoretical and experimental basis for the development of novel drugs. Wild YASH1 from the Loess Plateau in Yan'an region was collected, and polysaccharides and total flavonoids were extracted, isolated and identified from mycelium and sporophore.

The Role of IAA in Regulating Root Architecture of Sweetpotato ( [L.] Lam) in Response to Potassium Deficiency Stress.

Plants can adapt to the spatial heterogeneity of soil nutrients by changing the morphology and architecture of the root system. Here, we explored the role of auxin in the response of sweetpotato roots to potassium (K) deficiency stress. Two sweetpotato cultivars, Xushu 32 (low-K-tolerant) and Ningzishu 1 (low-K-sensitive), were cultured in low K (0.

The gut microbiota: A new perspective for tertiary prevention of hepatobiliary and gallbladder diseases.

The gut microbiota is a complex ecosystem that has coevolved with the human body for hundreds of millions of years. In the past 30 years, with the progress of gene sequencing and omics technology, the research related to gut microbiota has developed rapidly especially in the field of digestive system diseases and systemic metabolic diseases. Mechanical, biological, immune, and other factors make the intestinal flora form a close bidirectional connection with the liver and gallbladder, which can be called the "gut-liver-biliary axis.

Effects of phosphorus application on yield, quality and phosphorus use efficiency of edible sweetpotato: A case study of Xushu 32.

To explore the appropriate amount of phosphorus (P) fertilizer and improve economic yield and P use efficiency of edible sweetpotato, we took Xushu 32 as an example and compared the effects of different P application rates on yield, quality, P accumulation and P use efficiency of edible sweetpotato based on a two-year field experiment (soil available P content was 31.70 mg·kg) from 2018 to 2019. There were five P application levels (PO), including 0 (P), 25 (P), 50 (P), 75 (P) and 100 kg·hm(P).