Publications by authors named "Zhu Xiangqing"
Ovarian aging leads to endocrine disorders and systemic degeneration of tissue and organ structure and function, seriously affecting women's physical and mental health. Safe and effective treatments for this condition are lacking. Umbilical cord mesenchymal stem cells (UCMSCs), which have multidirectional differentiation potential, show strong self-renewal, secrete bioactive factors and release exosomes, can undergo homing, colonization, integration and differentiation into supporting and functional cells in tissues and organs through direct manipulation and can also improve the tissue microenvironment through paracrine action, promoting cell division, proliferation and microangiogenesis, inhibiting inflammation and apoptosis, reducing oxidative stress, and mediating two-way immune regulation.
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- Aging leads to various degenerative diseases, particularly affecting the skin, which undergoes significant structural and functional changes, such as reduced collagen production and increased wrinkles.
- Mesenchymal stromal cells (MSC), particularly those derived from umbilical cords (UC-MSC), show promise in cell therapy for delaying skin aging due to their regenerative properties.
- The paper aims to explore the mechanisms by which UC-MSCs contribute to skin rejuvenation, providing a foundation for future research in combating skin aging.
Article Synopsis
- Reduced thymic epithelial cells (TECs) are linked to thymic degeneration, but umbilical cord mesenchymal stem cells (UCMSCs) have shown promise in restoring TEC function and structure despite the unclear mechanisms behind this effect.
- The study involved inducing senescence in TECs and then coculturing them with UCMSCs, which resulted in significant changes in gene expression, particularly in the PI3K-AKT signaling pathway.
- The findings suggest that UCMSCs enhance TEC proliferation by upregulating VEGFA and promoting key cell cycle proteins, ultimately reversing age-related dysfunction in TECs.
Background: A decrease in the number and activity of thymic epithelial cells (TECs) is an important factor in thymic degeneration. Mesenchymal stem cells (MSCs) treating thymic ageing is a promising strategy, but the DNA methylation modification mechanism in TECs remains unclear.
Methods: Aged rhesus monkeys were treated with MSCs to establish a thymic senescence model, and hematoxylin-eosin (HE) staining, immunofluorescence staining, and ELISA were performed to observe the structure and function of the thymus.
An age classification model based on DNA methylation biomarkers of aging in human peripheral blood using random forest and artificial neural network.
Cell Mol Biol (Noisy-le-grand)
January 2024
Recent epigenetic studies have revealed a strong association between DNA methylation and aging and lifespan, which changes (increases or decreases) with age. Based on these, the construction of age prediction models associated with DNA methylation levels can be used to infer biological ages closer to the functional state of the organism. We downloaded methylation data from the Gene Expression Omnibus (GEO) public database for normal peripheral blood samples from people of different ages.
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Background: Osteoporosis increases bone brittleness and the risk of fracture. Umbilical cord mesenchymal stem cell (UCMSC) treatment is effective, but how to improve the biological activity and clinical efficacy of UCMSCs has not been determined.
Methods: A rat model of osteoporosis was induced with dexamethasone sodium phosphate.
Article Synopsis
- Recent studies indicate that umbilical cord mesenchymal stem cells (HA-MSCs) may counteract ovarian aging, although the specific cellular and molecular mechanisms are not yet fully understood.
- A 10X Genomics single-nucleus transcriptome sequencing experiment was conducted on the ovaries of macaque monkeys after HA-MSC treatment to explore these mechanisms.
- Results showed that HA-MSC treatment improved the structure and function of ovaries by enhancing cell proliferation and reversing senescence in aging cells, suggesting a promising new approach for treating ovarian aging.
Article Synopsis
- * Current treatments for cardiovascular diseases include drug therapies, which can have various side effects, and nondrug therapies like minimally invasive procedures and cell therapy, but traditional methods also have drawbacks such as cost and trauma.
- * Stem cell therapy has shown promising results in cardiovascular research due to its ability to regenerate and differentiate, with this paper reviewing recent advancements in this area and discussing future directions for treating aging-related heart issues in China.
Background: Ovarian ageing causes endocrine disturbances and the degeneration of systemic tissue and organ functions to seriously affect women's physical and mental health, and effective treatment methods are urgently needed. Based on our previous studies using juvenile rhesus monkey bone marrow mesenchymal stem cells (BMMSCs) to treat ovarian ageing in rhesus monkey, we found that BMMSCs improved ovarian structure and function. This study continues to explore the mechanism by which BMMSCs reversed granulosa cell (GC) ageing.
View Article and Find Full Text PDFObjective: The aim of the study was to reveal the changes in serum protein composition and content in macaques during the process of ageing, and explore the effect of bone marrow mesenchymal stem cell (BMMSC) on the serum protein expression profile in elderly macaques.
Methods: Naturally ageing macaques were assessed according to age. BMMSCs were intravenously infused into aged macaques.
Article Synopsis
- Senile thymus atrophy contributes to reduced immune function, making it important to repair thymus tissue and restore immune activity in the elderly.
- In a study, elderly macaques were treated with bone marrow mesenchymal stem cells, resulting in increased thymus volume, reduced fibrosis, and signs of tissue regeneration.
- Transcriptomic analysis revealed that after stem cell therapy, the thymus expression patterns in old macaques resembled those of younger ones, suggesting this therapy could prevent or reverse thymus aging.
Effects of umbilical cord mesenchymal stem cells on expression of CYR61, FSH and AMH in mice with premature ovarian failure.
Cell Mol Biol (Noisy-le-grand)
January 2022
The aim of this study was to objective to investigate the effects of umbilical cord mesenchymal stem cells on the expression of CYR61, FSH and AMH in mice with premature ovarian failure. For this purpose, thirty SPF female SD mice were selected as the research object, 10 of which were control group, namely group α, and 20 mice with premature ovarian failure model were established by cyclophosphamide. The mice were divided into model group, namely β group and the umbilical cord mesenchymal stem cell transplantation group (γ group), with 10 mice in each group.
View Article and Find Full Text PDFBackground: Intestinal disease is a common disease, which can cause serious digestion and absorption disorders, endanger the lives of patients and seriously affect the quality of life of people. Finding an effective treatment is a difficult problem at present, and stem cell therapy as a treatment has high application potential in intestinal-related diseases.
Purpose: This paper mainly summarizes the mechanism, research progress and future development trend of stem cells in the treatment of intestinal diseases in the past decade, hoping to provide a reference for future researchers in the research and application of stem cells and intestinal diseases.
Accumulated evidence shows that tumor microenvironment plays crucial roles in predicting clinical outcomes of lung adenocarcinoma (LUAD). The current study aimed to identify some potentially prognostic signatures by systematically revealing the transcriptome characteristics in LUADs with differing immune phenotypes. LUAD gene expression data were retrieved from the public TCGA and GEO databases, and the transcriptome characteristics were systematically revealed using a comprehensive bioinformatics method including single-sample gene set enrichment analysis, differentially expressed gene (DEG) analysis, protein and protein interaction (PPI) network construction, competitive endogenous RNA (ceRNA) network construction, weighted gene coexpression network analysis and prognostic model establishment.
View Article and Find Full Text PDFBackground: The tumorigenesis of infused umbilical cord mesenchymal stem cells (UC-MSCs) is being preclinically evaluated.
Methods: We observed tumor formation in NOD SCID mice after a single subcutaneous injection of hUC-MSCs and the effect of these cells on tumor growth in tumor-bearing mice. Three generations (P5, P7, and P10) of hUC-MSCs (1 × 10) from two donors (hUC-MSC1 and hUC-MSC2) were inoculated subcutaneously into NOD SCID mice.
As human life expectancy continues to increase and the birth rate continues to decline, the phenomenon of aging is becoming more prominent worldwide. Therefore, addressing the problems associated with global aging has become a current research focus. The main manifestations of human aging are structural degeneration and functional decline of aging tissues and organs, quality of life decline, decreased ability to resist diseases, and high incidence rates of a variety of senile degenerative diseases.
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- The study investigates the effects of mouse umbilical cord mesenchymal stem cells (mUCMSCs) on the reproductive health of ageing C57 mice, focusing on their potential to repair ovarian function.
- Aging mice with diminished ovarian function received mUCMSC injections, resulting in increased ovarian size, the presence of follicles, and enhanced hormone levels compared to a control group.
- The findings indicate that mUCMSCs can reduce cell apoptosis and oxidative stress in granulosa cells by altering specific gene expressions and activating key cellular pathways, suggesting a potential for regenerative therapies in reproductive health.
Article Synopsis
- Female sex hormone production and reproductive functions decline with age, and bone marrow mesenchymal stem cells (BMMSCs) may help treat this ovarian ageing.
- In experiments with macaque models, treatment with juvenile BMMSCs led to improvements in ovarian structure and function, including increased ovarian volume and better regulation of sex hormones.
- Gene analysis revealed that BMMSC treatment altered the expression of over 1,200 genes associated with ageing, suggesting pathways that could be targeted to reverse ovarian aging effects.
The effects of BMMSC treatment on lung tissue degeneration in elderly macaques.
Stem Cell Res Ther
March 2021
Article Synopsis
- Age-related degeneration of lung tissue increases the risk of severe lung diseases, making the search for anti-aging treatments crucial.
- A study on elderly macaque models found that administration of bone marrow-derived mesenchymal stem cells (BMMSCs) significantly improved lung health by reducing fibrosis and inflammation.
- BMMSC treatment was effective in decreasing harmful oxidative stress and enhancing the function and proliferation of lung cells, suggesting it could be a viable method to combat age-related lung degeneration.
Article Synopsis
- A mouse model for allergic rhinitis (AR) was developed using BALB/c mice to aid in future research on the condition.
- * The study involved preparing human umbilical cord mesenchymal stem cells (hUCMSCs), which were shown to be effective for tracking in animal studies and possibly for AR treatment.
- * The transplantation of hUCMSCs, particularly through tail vein injection, demonstrated positive effects on the AR model, with a significant presence of these cells in the nasal area after two weeks.
Article Synopsis
- Periodontitis, a chronic inflammatory disease caused by dental plaque, leads to a state of immune system hyporesponsiveness known as endotoxin tolerance, particularly involving neutrophils, the body's primary innate immune cells.
- In this study, researchers focused on how neutrophils respond to repeated exposures to Porphyromonas gingivalis lipopolysaccharide (LPS), measuring aspects like apoptosis, respiratory burst, cytokine production, phagocytosis, and the formation of extracellular traps.
- Findings revealed that LPS tolerance resulted in suppressed apoptosis, decreased production of pro-inflammatory cytokines like TNF-α, increased IL-10, and enhanced formation of neutrophil extracellular traps, suggesting a reprogramming of
Article Synopsis
- The study investigates early predictors for the recurrence and spread of rectal cancer after surgery, utilizing data from 400 patients operated on between September 2013 and September 2014.
- Key findings indicate that factors like tumor size on preoperative CT scans, lymphocyte-to-neutrophil ratio, and CEA levels are significant biomarkers for assessing the risk of recurrence or metastasis.
- A nomogram model developed from these predictors showed a high accuracy (AUC of 0.939) for forecasting post-operative outcomes, emphasizing the utility of these biomarkers in clinical assessments.
Article Synopsis
- Inflammatory bowel disease (IBD) causes chronic inflammation in the gastrointestinal tract, and researchers are exploring new treatments with minimal side effects.
- Mouse models of IBD were created using a solution called DSS, allowing scientists to test the effects of umbilical cord mesenchymal stem cells (UCMSCs) from both mice and humans.
- The study found that UCMSCs improve survival rates and promote healing by enhancing key proteins in the intestines, offering a promising avenue for future IBD therapies.