Publications by authors named "Zhu Quan"

Loop-extrusion machinery, comprising the cohesin complex and CCCTC-binding factor CTCF, organizes the interphase chromosomes into topologically associating domains (TADs) and loops, but acute depletion of components of this machinery results in variable transcriptional changes in different cell types, highlighting the complex relationship between chromatin organization and gene regulation. Here, we systematically investigated the role of 3D genome architecture in gene regulation in mouse embryonic stem cells under various perturbation conditions. We found that acute depletion of cohesin or CTCF disrupts the formation of TADs, but affects gene regulation in a gene-specific and context-dependent manner.

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We present the first use of a bioengineered mammalian transposase system derived from Myotis lucifugus (bMLT) for integration of expression vectors into the CHO genome, focusing on GFP and trastuzumab production. Initially, CHO-K1 cells are transfected with a GFP reporter and varying amounts of bMLT DNA or mRNA. GFP expression is monitored over 17 weeks without selective pressure.

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Background: The outcomes of segmentectomy for small (≤2 cm) non-small cell lung cancer (NSCLC) have been well demonstrated. This study aimed to investigate whether segmentectomy could achieve comparable oncologic outcomes with lobectomy for >2-3 cm lung cancer.

Methods: Patients who underwent segmentectomy or lobectomy for NSCLC >2-3 cm were retrospectively screened.

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Article Synopsis
  • The study examines the roles of the hippocampus and prefrontal cortex in learning and cognition, focusing on their molecular development through innovative genomic techniques.
  • Researchers used over 53,000 single-nucleus profiles to analyze DNA methylation and chromatin conformation changes, finding that these processes occur at different times during development.
  • The findings reveal distinct chromatin interactions in neurons versus glial cells and identify specific genetic variants associated with schizophrenia, highlighting the potential of single-cell multi-omics in understanding brain development and neuropsychiatric disorders.
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Diethoxymethane (DEM), a promising carbon-neutral fuel, has high reactivity at low temperatures. The intramolecular hydrogen migration reaction of the DEM peroxy radicals can be viewed as a critical step in the low temperature oxidation mechanism of DEM. In this work, multistructural transition state theory (MS-TST) was utilized to calculate the high-pressure limit rate constants of 1,5, 1,6 and 1,7 H-migration reactions for DEM peroxy radicals.

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Objective: To determine incidence and risk factors for venous thromboembolism (VTE) development of in-hospital VTE in urological inpatients who underwent non-oncological surgery in a tertiary hospital in China.

Methods: Consecutive 1453 inpatients who were admitted to a non-oncological urological ward in the tertiary hospital from January 1, 2018 to December 31, 2018 were enrolled in the study, and the VTE events were diagnosed by ultrasound or computed tomographic pulmonary angiography. Patients' occurrence of VTE and characteristics which may contribute to the development of VTE were collected and analyzed as incidence and risk factors.

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Article Synopsis
  • The study explores the effectiveness of a new self-designed stapler tractor in improving surgical outcomes for lung segmentectomy using the tunneling technique, which has potential advantages but faces challenges with staple passage.
  • In a retrospective analysis, patients were divided into four groups based on surgical approaches, with Group A (using the stapler tractor with tunneling) showing the best results in several aspects, including operation time and fewer complications.
  • The research concludes that the tunneling technique, when used with the stapler tractor, represents a significant advancement, leading to better surgical precision and shorter hospital stays compared to traditional methods.
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Introduction: Vitexin is a natural flavonoid compound extracted from Vitex leaves or seeds, exhibiting various pharmacological activities including anticancer, antihypertensive, anti-inflammatory, and spasmolytic effects. However, its protective effects on hypertensive nephropathy (HN) and the underlying mechanisms remain unclear.

Methods: Spontaneous hypertension rats were fed a high-sugar and high-fat diet for 8 weeks to induce the disease HN model.

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Article Synopsis
  • Cellular senescence, once thought to only occur in tissue cultures, is now recognized as playing complex roles in various biological processes across multiple species, including humans.
  • Traditional understanding of senescent cells primarily comes from lab studies, but these cells are rare in actual tissues, and fully developed cells can also show signs of senescence.
  • The SenNet Biomarkers Working Group has created recommendations for identifying senescent cells in tissues, analyzing literature on markers in mice and humans, and discussing new methods for detection that will assist researchers in the field.
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Background: Cystic lung cancer, a special type of lung cancer, has been paid more and more attention. The most common pathological type of cystic lung cancer is adenocarcinoma. The invasiveness of cystic lung adenocarcinoma is vital for the selection of clinical treatment and prognosis.

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Background: Lung segmentectomy has gained much more attention as an important surgical method for treating early-stage lung cancer. However, incomplete fissures increase the difficulty of lung segmentectomy. The aim of this study was to analyze the safety and efficacy of the fissure-first approach in precision resection of lung segments for patients with incomplete fissures.

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We investigate the reaction pathways of nine important CO-related reactions using the revDSD-PBEP86-D3(BJ)/jun-cc-pV(T+d)Z level and simultaneously employ an accurate composite method (jun-Cheap) based on coupled-cluster (CC) theory. Subsequently, the Rice-Ramsperger-Kassel-Marcus/master equation (RRKM/ME) is solved to calculate the temperature- and pressure-dependent rate constants. This work investigates reactions involving transition states that have been overlooked in previous literature, including the dissociation of singlet-state CO, the triple channel formation of CO + CO to form CO, and the formation of O + CO.

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. Automatic and accurate airway segmentation is necessary for lung disease diagnosis. The complex tree-like structures leads to gaps in the different generations of the airway tree, and thus airway segmentation is also considered to be a multi-scale problem.

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The spatial organization of molecules in a cell is essential for their functions. While current methods focus on discerning tissue architecture, cell-cell interactions, and spatial expression patterns, they are limited to the multicellular scale. We present Bento, a Python toolkit that takes advantage of single-molecule information to enable spatial analysis at the subcellular scale.

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Background: Ewing sarcoma is a rare tumor of the bone or soft tissues characterized by diffuse membranous staining for CD99. As this tumor remains incurable in the metastatic, relapsed, and refractory settings, we explored the downstream immune implications of targeting CD99.

Methods: We discovered a human anti-CD99 antibody (NOA2) by phagemid panning and investigated NOA2 immune cell-mediated cytotoxicity in vitro and in vivo focusing on the myeloid cell compartment, given that M2 macrophages are present in human tumors and associated with a poor prognosis.

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In recent years, hazardous chemical incidents have occurred frequently, resulting in significant human casualties, property damage, and environmental pollution due to human or natural factors. Accurately mining the lessons learned from accumulating incident reports and constructing the knowledge graph for hazardous chemical incident management can assist managers in identifying patterns and analyzing common attributes, thereby preventing the recurrence of similar incidents. This article addresses the challenges of dispersed textual information, specialized vocabulary, and data formats in hazardous chemical incidents.

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The heart, which is the first organ to develop, is highly dependent on its form to function. However, how diverse cardiac cell types spatially coordinate to create the complex morphological structures that are crucial for heart function remains unclear. Here we integrated single-cell RNA-sequencing with high-resolution multiplexed error-robust fluorescence in situ hybridization to resolve the identity of the cardiac cell types that develop the human heart.

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Background: Monosubsegmentectomy (MSS) involves removal of less lung parenchyma than monosegmentectomy (MS) does. However, the clinical efficacy of MSS in lung cancer treatment remains unclear, with concerns regarding insufficient surgical margins and increased complications.

Methods: Between February 2015 and December 2019, patients who underwent thoracoscopic MSS (n = 126) or MS (n = 678) for small ground-glass opacity (GGO) dominant lung cancer were examined.

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Objectives: Human leukocyte antigen (HLA) B27 is a susceptibility allele of ankylosing spondylitis (AS), and HLA-B27 antigen typing is an important indicator for clinical diagnosis of AS, but current typing methods such as sequence specific primer polymerase chain reaction (PCR-SSP) still possess limitation. Therefore, this study aims to analyze the correlation between B27 subtypes and susceptibility to AS in Hunan Province by applying high-resolution polymerase chain reaction-sequence-based typing (PCR-SBT).

Methods: Peripheral blood of 116 patients with suspected AS (suspected AS group) and 121 healthy volunteers (control group) admitted to the Second Xiangya Hospital from January 2020 to December 2020 were collected for HLA-B genotyping by PCR-SBT.

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Purpose: Early-stage lung cancer is typically characterized clinically by the presence of isolated lung nodules. Thousands of cases are examined each year, and one case usually contains numerous lung CT slices. Detecting and classifying early microscopic lung nodules is demanding due to their diminutive dimensions and restricted characterization capabilities.

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LILRB4, a myeloid inhibitory receptor belonging to the family of leukocyte immunoglobulin-like receptors (LILRs/LIRs), plays a pivotal role in the regulation of immune tolerance. LILRB4 primarily mediates suppressive immune responses by transmitting inhibitory signals through immunoreceptor tyrosine-based inhibitory motifs (ITIMs). This immune checkpoint molecule has gained considerable attention due to its potent regulatory functions.

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Article Synopsis
  • The study addresses a key challenge in treating solid tumors with CAR T cells, specifically the negative effects of the tumor microenvironment (TME).
  • Researchers created a modified CAR T cell (G36-PDL1) that not only targets a protein associated with tumors (carbonic anhydrase IX or CAIX) but also secretes an antibody to counteract immune suppression.
  • In tests using a mouse model of kidney cancer, the G36-PDL1 CAR T cells showed strong anti-tumor effects by enhancing immune responses and reducing suppressive cells in the TME compared to standard CAR T cells.
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Purpose: Intraoperative frozen section pathology (FS) is widely used to guide surgical strategies while the accuracy is relatively low. Underestimating the pathological condition may result in inadequate surgical margins. This study aims to identify CT imaging features related to upgraded FS and develop a predictive model.

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