Antigenicity and genetic properties of an Eurasian avian-like H1N1 swine influenza virus in Jiangsu Province, China.

Pigs are vital genetic mixing vessels for human and avian influenza viruses because their tracheal epitheliums possess both sialic acid α-2,6-Gal and α-2,3-Gal receptors. Cross-species transmission of influenza A viruses from swine to humans occurs occasionally. The first case of human infection with the Eurasian avian-like H1N1 swine influenza virus (EAH1N1 SIVs) genotype G4 was detected in Jiangsu Province, China, in February 2023, and backtracking epidemiological investigations did not reveal a clear source of the infection.

Long term gait postural characteristics of children with general foot pain using smartphone connected wearable sensors.

Whether children's general foot pain related to specific foot posture remains unknown. The aim of the study was to determine long-term dynamic foot posture characteristics in children with general foot pain using smartphone-connected wearable sensors. Over a 30-day period, 94 children (37 girls and 57 boys, mean age 9.

[Clinical study on the effectiveness of bone acupuncture for alleviating pain and improving function in patients with degenerative lumbar spinal stenosis].

Objective: To assess the effectiveness of bone acupuncture in improving pain and function in degenerative lumbar spinal stenosis (DLSS) and compare it with acupuncture.

Methods: From January to December 2023, 80 DLSS patients were treated with acupuncture and divided into bone acupuncture and acupuncture groups. Among them, 40 patients in the bone acupuncture group included 15 males and 25 females, with a mean age of (60.

The influence of different stress loading on the biomechanics of motion segments in isolated rabbit spines.

Intervertebral disc degeneration greatly affects daily life. Suitable mechanical stress is important for intervertebral disc health as it affects disc cells. Research shows it helps disc cell proliferation and collagen synthesis.

Yishenyangsui granule for degenerative cervical myelopathy: a randomized, double-blind, placebo-controlled trial with long-term follow-up.

Objective: This randomized controlled trial aims to evaluate the efficacy and safety of Yishenyangsui granule for treating Degenerative Cervical Myelopathy.

Materials And Methods: A randomized, double-blind, placebo-controlled clinical trial was conducted with 152 participants recruited from three centers and randomly assigned to receive either Yishenyangsui granule or placebo. The Japanese Orthopaedic Association (JOA) score and Neck Disability Index (NDI) score were evaluated for 32 weeks.

Evolutionary dynamics of SARS-CoV-2 variants under strict non-pharmaceutical interventions - a population-based study.

Introduction: China implemented a dynamic zero-COVID strategy to curb viral transmission in response to the coronavirus disease 2019 (COVID-19) pandemic. This strategy was designed to inhibit mutation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for COVID-19. This study explores the dynamics of viral evolution under stringent non-pharmaceutical interventions (NPIs) through real-world observations.

Single-Cell Transcriptomic Analysis Reveals Biomechanical Loading-Induced Imbalance in Bone and Fat, Leading to Ossification in Lumbar Intervertebral Disc Nucleus Pulposus Degeneration.

In this study, we explored the impact of different biomechanical loadings on lumbar spine motion segments, particularly concerning intervertebral disc degeneration (IVDD). We aimed to uncover the cellular milieu and mechanisms driving ossification in the nucleus pulposus (NP) during IVDD, a process whose underlying mechanisms have remained elusive. The study involved the examination of fresh NP tissue from the L3-S1 segment of five individuals, either with IVDD or healthy.

Therapeutic effects and mechanisms of Juanbilijieqing fang in ameliorating gouty arthritis in a murine model.

This study aims to assess the safety, efficacy, and mechanisms of Juanbilijieqing Fang in a mouse model of gouty arthritis. C57BL/6 mice were allocated into six groups: control, gouty arthritis model, and treatment groups receiving varying doses of Juanbilijieqing Fang (low, medium, high), along with a positive control group treated with febuxostat. Gouty arthritis was induced via MSU crystal injection following a high-fat diet.

Contradictory links between lipid levels and bone health: atherogenic index of plasma and bone microarchitecture.

Purpose: Lipids increase osteoporosis and fracture risk, yet research on Atherogenic Index of Plasma (AIP), bone mineral density declines (BMD), and trabecular bone score (TBS) is limited. This study examined the relationships between AIP, TBS, and BMD in American adults using the National Health and Nutrition Examination Survey (NHANES) data, including the effect of obesity.

Methods: Based on data from 3,162 Americans, multivariable logistic regression and a generalized additive model (GAM) were used to analyze AIP, BMD, and TBS.

Dynamic Changes and Trends of SARS-CoV-2 Antibodies Induced by Infection and Vaccination Across Multiple Time Points.

As the COVID-19 pandemic continues, increasingly complex vaccination and infection histories have made it urgent to investigate the antibody dynamics in populations with hybrid immunity. This study aimed to explore the multi-time-point dynamics of SARS-CoV-2 IgG antibody levels in a community-based population in Jiangsu Province, China, following the Omicron BA.5 wave, as well as the long-term persistence of IgG antibodies nearly 2 years postinfection.

Non-linear association of sleep duration with osteoarthritis among U.S. middle-aged and older adults.

Background: The duration of sleep is linked to a range of disorders. Osteoarthritis (OA) stands as one of the most prevalent forms of arthritis and serves as a leading cause of disability. The correlation between the duration of sleep and OA remains ambiguous.

[Mechanism of aucubin in regulating ribosome biogenesis and inhibiting injury of nucleus pulposus cells and extracellular matrix degradation].

This study aimed to investigate the effect of aucubin(AU) on injury of nucleus pulposus cells and extracellular matrix(ECM) degradation and its mechanism. The nucleus pulposus cell injury model was established by interleukin-1β(IL-1β) and treated with AU or phosphatidylinositol 3-kinase(PI3K) inhibitor LY294002. CCK-8 experiment was conducted to test cell proliferation.

[Quality assessment of animal experimental studies on traditional Chinese medicine treatment of cervical radiculopathy].

This study aims to assess the methodological and reporting quality of animal experimental studies on the treatment of cervical radiculopathy(CR) with traditional Chinese medicine(TCM), analyze the deficiencies during the experimental process, and develop the methods to enhance the quality of such studies. The related articles were retrieved from CNKI, Wanfang, VIP, SinoMed, PubMed, EMbase, Cochrane Library, and Web of Science. The methodological quality and reporting quality of the included studies were evaluated according to the risk of bias tool of the Systematic Review Centre for Laboratory Animal Experimentation(SYRCLE) and the Animal Research: Reporting of in vivo Experiments(ARRIVE) 2.

[Strategies for prevention and treatment of spinal degenerative diseases from perspective of traditional Chinese medicine for bone health].

The population aging and the coming of the information era are accompanied with the growing incidence of spinal degenerative diseases, which result in heavy social and economic burdens. Under the guidance of the tendon-bone theory, rich experience has been accumulated in the prevention and diagnosis of spinal degenerative diseases with traditional Chinese medicine(TCM), which demonstrates unique advantages. China's government has placed people's health in the strategic position of development, providing a favorable environment for the realization of healthy aging.

[Expert consensus on clinical application of Dieda Qili Tablets in treatment of acute soft tissue injury].

Dieda Qili Tablets are protected traditional Chinese medicine(TCM) variety and type B medicine included in medical insurance in China, which is widely used in the treatment of acute soft tissue injury in clinical practice and has been recommended by many books. However, there is no evidence-based guideline or consensus to guide the clinical application of Dieda Qili Tablets. With the support of China Association of Chinese Medicine, Wangjing Hospital of China Academy of Chinese Medical Sciences led the 21 Chinese units to follow the guiding ideology of "evidence as the key, consensus as the supplement, and experience as the reference" to compile this consensus.

Cervical Rotation-Traction Manipulation for Cervical Radiculopathy: A Systematic Review and Meta-Analysis of Randomized Control Trials.

Background: Cervical radiculopathy (CR) is a common musculoskeletal disorder worldwide. Cervical rotation-traction manipulation (CRTM) is one of the representative technique in traditional Chinese orthopedics.

Objective: This systematic review and meta-analysis aims to evaluate the effectiveness and safety of CRTM in treating CR.

Neutralizing Antibody-Mediated Protection from Prior Delta Variant Infection Against Omicron BA.5 Sub-Lineage Reinfection One Year Later: A Prospective Cohort Study.

Background: Previous SARS-CoV-2 infection provides some level of protection against reinfection. However, few studies have evaluated the neutralizing antibody (NAb) response after Delta variant infection and its ability to prevent reinfection with Omicron BA.5 one year later.

Quantitative method for measuring fragment velocity using terahertz wave Doppler interferometric velocimetry.

A method of quantitatively measuring fragment velocity based on a terahertz wave Doppler interference velocimetry (TDV) system is proposed in this paper. The theoretical model of fragment group velocity measurement is established, and the quantitative extraction method of fragment group initial velocity, velocity attenuation coefficient, and fragment velocity or distance change with time are given. Using this model, the extracted maximum and minimum values of the fragment group's initial velocity and attenuation coefficient are in good agreement with the preset simulated values.

Age-Related Association Between Unilateral Single-Channel and Double-Channel Surgery and Postoperative Multifidus Muscle Atrophy and Fat Infiltration.

Background: Unilateral biportal endoscopic laminotomy (UBE) and percutaneous endoscopic laminotomy (PEL) are minimally invasive spinal surgery (MISS) techniques used for unilateral and dual-channel endoscopic laminectomy. However, limited research has been conducted on lumbosacral multifidus muscle injuries in elderly individuals undergoing MISS for lumbar spinal canal stenosis. The objective of this study was to investigate the impact of single-channel and double-channel MISS on the multifidus muscle in elderly patients.

Stability assessment of housekeeping genes for qRT-PCR in cultured at 22°C and 37°C.

, a species within the genus , thrives optimally at 22-25°C but can also grow at the mammalian core body temperature of 37°C. This dual temperature adaptability necessitates establishing both temperature conditions in research to examine the effects on various biological processes. In quantitative real-time PCR (qRT-PCR) assays, the selection of appropriate housekeeping genes is vital for data accuracy.